De Clerck, O.

Hall-Spencer, J.

Le Gall, L.

Milazzo, M.

Pena, V.

Range, P.

European Journal of Phycology

Peña, Viviana

Range, Pedro

Marco, Milazzo

de Clerck, Olivier

Le Gall, Line

Hall-Spencer, Jason M.

HAL Descartes

Assessment of coralline algal species diversity and composition at European CO2 seeps using DNA bar coding

Open Marine Archive

Marine benthic (sand-dwelling and epiphytic) dinoﬂa-
gellates consists of known potentially toxic species,
which are harmful to marine organisms as well as
human by consumption of sea food in mostly tropical
and subtropical region. Occurrence and distribution of
these species are less studied from temperate region.
The benthic dinoﬂagellates in the intertidal zone along
the coasts of Jeju Island was determined by monthly
collection of sand sediment and macroalgae samples
from eight sampling locations from March 2011 to
February 2012. Thirty-seven dinoﬂagellate taxa belong
to eighteen genera were identiﬁed; twenty six found
only in sand sediment, seven in macroalgae samples
and four in both samples. The most frequently occur-
ring species was Amphidinium carterae, followed byA.
operculatum, Coolia malayensis, andOstreopsis ovata,
occurred at all sampling stations. G. yasumotoi and
Gambierdiscus sp. were rarely occurred. The seasonal
abundance of epiphytic dinoﬂagellates was also quan-
titatively estimated by monthly collection of macroal-
gae samples (Rhodophyta, Phaeophyta, and
Chlorophyta) from six sampling locations from July
2012 to June 2013. Ten epiphytic dinoﬂagellate taxa,
including eight potentially toxic species Amphidinium
carterae, A. operculatum, Gambierdiscus sp.,
Ostreopsis ovata, Prorocentrum concavum, P. emargi-
natum, P. lima, and P. rhathymum, were identiﬁed. A
signiﬁcant change in seasonal abundance was recorded
with maximum (751.82±223.12 cells g−1 wet weight of
algae; cells g−1 hereafter) in June, followed by October
(650.45±225.02 cells g−1) and September (598.02
±197.82 cells g−1). O. ovata was the most abundant
(338.21±11 cells g−1) in October and Gambierdiscus
sp. was the least abundant only in September (6.92
±16.97 cells g−1) and October (average 6.54±6.54
cells g−1) at Hamduk. Signiﬁcantly, highest spatial
abundance of total dinoﬂagellates for all sampling sta-
tions was found at Hamduk (547.91±315 cells g−1),
while it was lowest at Hwasun (232.59±144.93 cells
g−1). Generally, each of the epiphytic dinoﬂagellates
did not show speciﬁc preference of macroalgae as host.
10PO.14
ASSESSMENT OF CORALLINE ALGAL
SPECIES DIVERSITYANDCOMPOSITION AT
EUROPEAN CO2 SEEPS USING DNA
BARCODING
Viviana Peña1 (vpena@udc.es), Pedro Range2 (pran
ge@qu.edu.qa), Marco Milazzo3 (marco.milazzo@
unipa.it), Olivier De Clerck4 (olivier.declerck@ugent.
be), Line Le Gall5 (legall@mnhn.fr) and Jason M
Hall-Spencer6 (jason.hall-spencer@plymouth.ac.uk)
1BIOCOST Research Group, Universidade da
Coruña, A Coruña 15071, Spain; 2Department of
Environmental and Biological Sciences, Qatar
University, Doha 2713, Qatar; 3CoNISMa,
Dipartimento di Scienze della Terra e del Mare,
Universitá di Palermo, Palermo 90123, Italy;
4Phycology Research Group, Ghent University, Ghent
9000, Belgium; 5Equipe Exploration, Espèces et
Evolution, Institut de Systématique, Evolution,
Biodiversité, UMR 7205 ISYEB CNRS, MNHN,
UPMC, EPHE, Muséum national d’Histoire naturelle
(MNHN), Sorbonne Universités, Paris 75005, France
and 6School of Marine Science and Engineering,
Plymouth University, Plymouth PL4 8AA, United
Kingdom
Coralline red algae are one of the most abundant
groups of benthic seaweeds throughout the photic
zone. In Europe, they are ecosystem engineers of
high-diversity habitats such as maerl beds, coralligen-
ous, vermetid reefs/trottoir. A major driver of change
in coastal ecosystems is the rise of CO2 levels which is
warming surface waters and reducing the amount of
carbonate available to calciﬁed organisms. Work at
volcanic seeps has shown that CO2 is a resource for
certain seaweeds but that carbonation causes dissolu-
tion of calciﬁed organisms. Volcanic seeps are starting
to be used as natural analogues for a high CO2 world,
but so far assessments of diversity of coralline algae
around those seeps have not been studied in detail. We
present the ﬁrst study focused on coralline species
diversity and composition along well constrained
CO2 gradients in two naturally acidiﬁed sites in
Europe: Vulcano- Italy- in the Mediterranean, and
Sao Miguel - Azores- in the Atlantic. Intertidal and
subtidal transects were carried out along established
CO2 gradients. Both cover and habit for each coralline
species were recorded, and samples were collected to
be identiﬁed using molecular (DNA barcoding) and
morphological (SEM) approaches. In addition, ele-
mental analyses of specimens were examined using
Energy-dispersive X-ray spectroscopy technique
(EDS). Results obtained in terms of potential changes
in species diversity and composition will be dis-
cussed. From an evolutionary perspective, our study
could shed some light on which coralline lineages
might be able to persist on low pH environments
given the records of paleo-acidiﬁcation events since
the origin of this group.
10PO.15
REALTIME IN SITUMONITORING OF
TOXIC ALGAE
Linda K Medlin1 (lkm@mba.ac.uk), Jahir Orozco2
(jahirorozcoholguin@mircrobiaenvironnement.eu),
Elise Villa2 (elise.villa@microbiaenvironnement.
com) and Delphine Guillebault2 (delphine.
Guillebault@microbiaenvironnement.com)
Posters 197


Assessment of coralline algal species diversity and composition at European CO<sub>2</sub> seeps using DNA barcoding

Assessment of coralline algal species diversity and composition at European CO<sub>2</sub> seeps using DNA barcoding

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