N-glikozilacija imunoglobulina G u kroničnoj bolesti presatka protiv primatelja nakon presadbe alogeničnih matičnih krvotvornih stanica [N-glycosylation of immunoglobulin G in chronic graft-versus-host disease]
Chronic graft-versus-host disease (cGvHD) is a systemic alloimmune and autoimmune disorder and the major late complication and leading cause of non-relapse morbidity and mortality after allogeneic hematopoietic stem cell transplantation (alloHSCT). The disease is characterized by an altered homeostasis of the humoral immune response. Immunoglobulin G (IgG) glycoprotein is the main effector molecule of the humoral immune response. Changes in IgG glycosylation are associated with a number of autoimmune diseases.
IgG glycosylation analysis was done on two populations, American (213 cGvHD patients) and Croatian (30 cGvHD patients and 30 controls (after alloHSCT, without cGvHD)). The American population results showed significant correlation with cGvHD NIH joint/fascia and skin score, disease activity and intensity of systemic immunosuppression. ROC analysis confirmed that IgG glycosylation increases specificity and sensitivity of models using laboratory parameters and markers of inflammation. The Croatian logistic regression model built using glycan and laboratory parameters singled out glycopeptides for which it is justified to assume that they significantly contribute to the cGvHD discrimination.
This study has shown that IgG glycosylation plays a significant role in cGvHD pathology, and that further research could contribute to the understanding of the disease biology and lead to the necessary biomarker of chronic GVHD
