The decomposed body of a male infant was found in an oil palm estate in Rengit, Batu Pahat, Johor wrapped with clothes. Entomological specimens recovered in this case were flesh fly larvae, Sarcophaga (Liosarcophaga) dux Thomson and Sarcophaga peregrina (Robineau-Desvoidy) (Diptera: Sarcophagidae). Minimum post mortem interval (PMImin) was approximately 48 hrs based on the largest specimen, i.e. S. dux. This species has been featured as forensically important species in Malaysia but scarcity of developmental data and bionomics in cadaver environment limit its utilization in forensic case. Issues pertaining its evidential value in this case and recommendations are described herein

Muhammad Hilmi Baba,

Raja Muhammad Zuha Raja Kamal Bashah,

UKM Journal Article Repository

  Serangga 21(2): 163-170 ISSN 1394-5130 © 2016, Centre for Insects Systematic,  Universiti Kebangsaan Malaysia        SARCOPHAGA DUX THOMSON (DIPTERA: SARCOPHAGIDAE) RECOVERED FROM WRAPPED BODY OF AN INFANT: ITS EVIDENTIAL VALUE  Raja Muhammad Zuha & Muhammad Hilmi Baba Forensic Science Programme, School of Diagnostic and Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia Corresponding author: rmzuha@ukm.edu.my   ABSTRACT  The decomposed body of a male infant was found in an oil palm estate in Rengit, Batu Pahat, Johor wrapped with clothes. Entomological specimens recovered in this case were flesh fly larvae, Sarcophaga (Liosarcophaga) dux Thomson and Sarcophaga peregrina (Robineau-Desvoidy) (Diptera: Sarcophagidae). Minimum post mortem interval (PMImin) was approximately 48 hrs based on the largest specimen, i.e. S. dux. This species has been featured as forensically important species in Malaysia but scarcity of developmental data and bionomics in cadaver environment limit its utilization in forensic case. Issues pertaining its evidential value in this case and recommendations are described herein.  Keywords: forensic entomology, post mortem interval, Sarcophagidae, flesh fly   ABSTRAK  Mayat bayi lelaki reput dijumpai di sebuah estet kelapa sawit di Rengit, Batu Pahat, Johor, dibungkus dengan pakaian. Spesimen entomologi yang dijumpai dalam kes ini terdiri daripada larva lalat daging, Sarcophaga (Liosarcophaga) dux Thomson dan Sarcophaga peregrina (Robineau-Desvoidy) (Diptera: Sarcophagidae). Selang masa pasca kematian minimum (PMImin) adalah 48 jam berdasarkan spesimen yang paling besar, i.e. S. dux. Spesies ini telah direkodkan sebagai spesies berkepentingan forensik di Malaysia tetapi kekurangan data perkembangan dan bionomik di persekitaran mayat mengehadkan aplikasinya dalam kes forensik. Isu dan syor berkenaan nilai bukti dalam kes ini turut dihuraikan.  Kata kunci: entomologi forensik, selang masa pasca kematian, Sarcophagidae, lalat daging  INTRODUCTION  The flesh flies (Diptera: Sarcophagidae) have been featured in forensic cases including in Malaysia. This fly family can be considered as the second most important group breeding on human remains after the blow flies (Diptera: Calliphoridae) (Byrd & Castner 2010). However, similar appearances of larvae from different species had caused difficulty in identification (Sukontason et al. 2010) and taxonomic identification mostly depends on the male genitalia (Marshall 2012). In recent years, interests have been shifted to Sarcophagidae and development of simplified taxonomic keys have becoming helpful to identify Sarcophagidae especially in Malaysia and neighboring countries (Sukontason et al. 2010; Samerjai et al. 2014; Heo et al. 2015). The forensically important species of Sarcophagidae in Malaysia is currently represented by 17 species, based on their occurrences on  decomposed  animal tissues and  human  remains,  including  164 Serangga   Sarcophaga (Liosarcophaga) dux Thomson (Tan et al. 2010). It is a species of medical importance and its distribution is not limited to the Oriental region (Sukontason et al. 2014). It has been recorded causing aural myiasis on human in Thailand (Sukontason e al. 2014) and parasitized scorpions in China (Shi et al. 2015). In Malaysia, S. dux has been featured in forensic cases (Nazni et al. 2015) including the current case being presented herein. Developmental data of S. dux in this region is scarce and it is the main purpose of this paper to further highlight the importance of this species as an important evidence in forensic entomology.  Case Background On 22 April 2016 at approximately 0830 hrs, a body of male infant was found in an oil palm estate in Rengit, Batu Pahat Johor. An autopsy was performed on 23 April 2016 at 1130 hrs and entomological specimens were collected during the autopsy. From the forensic pathologist’s observation, the body was in a state of active decomposition with considerably minimal infestation of fly larvae observed inside the clothes that wrapped the body. Entomological specimens, consist of 2 preserved larvae and 3 larvae were sent to Forensic Entomology Laboratory, Faculty of Health Sciences, Universiti Kebangsaan Malaysia on 25 April 2016. Upon receiving the specimens, a chain of custody voucher was provided to the sender. In the laboratory, live specimens were transferred in a plastic rearing container half-filled with sawdust and ≈70 g cow’s liver as food for larvae. The specimens were reared until adult stage to facilitate species identification. The preserved larvae were used as indicators for PMImin estimation. Length of each specimen was measured by using stereomicroscope fitted with DinoLite digital camera and DinoCapture software. After measuring the length, larvae were processed and mounted on slides for species identification. It was found that the preserved larvae consist of S. dux and Sarcophaga peregrina (Robineau-Desvoidy). Adult male sarcophagids reared from larvae were all identified as S. dux. Identification of both species based on larvae and adult specimens was conducted based Raja & Muhammad  165   on descriptions of local Sarcophagidae (Sukontason et al. 2010; 2014; Heo et al. 2015).   PMImin estimation was based on the age of the oldest insect specimens found on the body. In this investigation, the life cycle of the largest larva i.e. S. dux (third instar, body length = 16.9 mm) was used as reference. At environmental temperature of 35.2 – 37.9ºC, this species took approximately 48 hrs to reach almost similar length as recorded by Sukontason et al. (2010). Based on Sukontason et al. (2010) the maximum length that could be achieved by S. dux larva was nearly 18 mm. Environmental temperatures 3 days prior to body discovery in Rengit from local meteorological station were estimated almost similar to study by Sukontason et al. (2010), i.e. 35.0 – 36.0ºC. In retrospective, infestation of S. dux larva on the body might have begun on 21 April 2016.   At 35ºC, total larva duration for S. peregrina was 5 days (Villet et al. 2006) but the specimen (third instar, body length = 10.46 mm) was possibly half from its total developmental milestone. It has been recorded that S. peregrina larvae could reach 24.36±0.82 mm during its third instar post feeding stage when reared at 25±5ºC (Majumder et al. 2012). Due to limited number of specimens provided for the analysis and the scarcity of S. peregrina developmental data, PMImin estimation was based on the age estimation of S. dux larva.   Several limitations were noted in this analysis. Firstly, the number of specimens received in this case could be considered inadequate to represent overall life cycle of insects occurred on the body. The PMImin was estimated based on the age of single S. dux. For future work, it is recommended that if larvae on the corpse are fewer than 100, all should be sampled for analysis (Amendt et al. 2007). Secondly, the arrival of S. dux was possibly delayed by the condition of the body being wrapped in clothes. Such condition could affect period of insect activity (PIA) that usually  correlates  with  estimation  of  PMImin (Tomberlin et al.  166 Serangga   2011). After evaluating this condition, it was possible that the adult S. dux larviposit on the surface of the clothes and the larvae succeeded development in the concealed parts of the body. This might explain why in this case there were no representatives from Calliphoridae that normally oviposit directly on human remains. In tropical environment, wrapping of decomposing remains could delay invasion of blow flies up to 2.5 days (Goff 1992). Therefore, studies focusing on the PIA on bodies in concealed environments can provide better understanding on the relationship between insect colonization and PMImin.   In addition to the above, and thirdly, viviparity or ovoviviparity in S. dux is poorly studied. It is generally known that almost all Sarcophagidae are either obligate oviviparity or multilarviparity (Meier et al. 1999). Sarcophaga dux has been documented performing larviposition (Kumara et al. 2013) but in rare occasion, it has been observed to lay eggs (possibly ovoviviparity) in laboratory environment (Sukontason et al. 2010; 2014). By having this ability to incubate eggs or retain larvae longer in the uterus, PMImin estimation based on the age of the larvae could be inaccurate as the precocious development or internal incubation period was not considered (Villet et al. 2010)    This report highlights the occurrence of S. dux in a death investigation and issues pertaining its evidential value. It is likely this species could be featured as forensic evidence in the future. Therefore, further studies on S. dux focusing the aspect of sampling, PIA and development can help to improve its evidential value in forensic entomology.   ACKNOWLEDGEMENT  Authors would like to thank Dr. Nurul Ezzati Abdul Karim of Forensic Department, Hospital Sultanah Nora Ismail, Batu Pahat, Johor for providing the entomology specimens. We also appreciate the assistance from Inspector Nur Hidayah Mustafa  Raja & Muhammad  167   and Sergeant Musa Dasang of Crime and Investigation Division, IPD Batu Pahat, Johor, in this case.      REFERENCE  Amendt, J., Campobasso, C. P., Gaudry, E., Reiter, C. LeBlanc, H. N. & Hall, M. J. R. 2007. Best practice in forensic entomology--standards and guidelines. International Journal of Legal Medicine, 121: 90–104. Byrd, J. H., & Castner, J. L. 2010. Insects of forensic importance, p. 39. In Byrd, J.H., Castner, J.L. (eds.), Forensic Entomology: Utility of Arthropods in Legal Investigation. Boca Raton: CRC Press. Goff, M. L. 1992. Problems in estimation of postmortem interval resulting from wrapping of the corpse - a case-study from Hawaii. Journal of Agricultural Entomology, 9(4): 237–243. Heo, C. C., Latif, B., Silahuddin, S. A. & Kurahashi, H. 2015. Morphology of third instar Boettcherisca highlandica Kurahashi & Tan 2009 (Diptera: Sarcophagidae). Halteres, 6: 121–131. Kumara, T. K., Abu Hassan, A., Che Salmah, M. R. & Bhupinder, S. 2013. Larval growth of Liosarcophaga dux Thompson (Diptera: Sarcophagidae) under uncontrolled indoor temperature in Malaysia. Southeast Asian Journal of Tropical Medicine and Public Health. 44(2): 182–187. Majumder, M. Z. R., Dash, M. K., Khan, R. A. & Khan, H. R. 2012. The biology of flesh fly, Boettcherisca peregrina (Robineau-Desvoidy, 1830) (Diptera: Sarcophagidae). Bangladesh Journal of Zoology, 40: 183–196. Marshall, S. A. 2012. Flies: The natural history and diversity of Diptera, First. Ed. New York: Firefly Books. 168 Serangga    Meier, R., Kotrba, M. & Ferrar, P. 1999. Ovoviviparity and viviparity in the Diptera. Biological Reviews, 74: 199–258. Nazni, W. A., Lee, H. L., Chew, W. K., Ali, R., Jeffery, J. & Heo, C. C. 2015. Chapter 3. Malaysia, pp. 19–35. In Tomberlin, J.K., Benbow, M.E. (eds.), Forensic Entomology: International Dimensions & Frontiers. Boca Raton: CRC Press. Samerjai, C., Sanit, S., Sukontason, K., Klong-klaew, T., Kurahashi, H., Tomberlin, J. K., Morakote, N., Wannasan, A. & Sukontason, K. L. 2014. Morphology of puparia of flesh flies in Thailand. Tropical Biomedicine, 31: 351–361. Shi, C. M., Zhang, X. S. & Zhang, D. X. 2015. Parasitoidism of the Sarcophaga dux (Diptera: Sarcophagidae) on the Mesobuthus martensii (Scorpiones: Buthidae) and its implications. Annals of the Entomological Society of America, 108(6): 978–985. Sukontason, K., Bunchu, N., Chaiwong, T., Moopayak, K. & Sukontason, K. L. 2010. Forensically important flesh fly species in Thailand: Morphology and developmental rate. Parasitology Research, 106: 1055–1064. Sukontason, K. L., Sanit, S., Klong-klaew, T., Tomberlin, J. K. & Sukontason, K. 2014. Sarcophaga (Liosarcophaga) dux (Diptera: Sarcophagidae): A flesh fly species of medical importance. Biological Research 47: 14. Tan, S. H., Rizman-Idid, M., Mohd-Aris, E., Kurahashi, H. & Mohamed, Z. 2010. DNA-based characterisation and classification of forensically important flesh flies (Diptera: Sarcophagidae) in Malaysia. Forensic Science International, 199: 43–49. Raja & Muhammad  169    Tomberlin, J. K., Mohr, R., Benbow, M. E., Tarone, A. M. & Van Laerhoven, S. 2011. A roadmap for bridging basic and applied research in forensic entomology. Annual Review of Entomology, 56: 401–421. Villet, M. H., Mackenzie, B. & Muller, W. J. 2006. Larval development of the carrion-breeding flesh fly, Sarcophaga (Liosarcophaga) tibialis Macquart (Diptera: Sarcophagidae), at constant temperatures. African Entomology, 14(2): 357–366. Villet, M. H., Richards, C. S. & Midgley, J. M. 2010. Contemporary, precision, bias and accuracy of minimum post-mortem intervals estimated using development of carrion-feeding insects, Chapter 7 pp. 109–137. In Amendt, J., Goff, M.L., Campobasso, C.P., Grassberger, M. (eds.), Current Concepts in Forensic Entomology. Dordrecht: Springer.      170 Serangga 

Sarcophaga dux Thomson (Diptera: Sarcophagidae) recovered from wrapped body of an infant: its evidential value

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