Chu, Wan Loy

Dang, Nguk Ling

Kok, Yih Yih

Phang, Siew Moi

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Dang Nguk Ling'Chu Wan Loy,,2Kok Yih Yih2Phang Siew Moi3'School of Postgraduate Studiesand Research, InternationalMedical University, No. 126,Jalan 19/155B, Bukit JaW,57000 Kuala Lumpur, Malaysia2Division of Human Biology,International MedicalUniversity, Bukit Jalil, 57000Kuala Lumpur, Malaysia31nstitute of Biological Sciences& Institute of Ocean and EarthSciences, University ofMalaya, 50603 Kuala Lumpur,MalaysiaInfluence of copper on cell v~abilit_Yand reactive o~ygen sp.ecies(ROS) production in the marme microalgae Dunoliella tertiolecta andPavlova gyransThere has been increasing concern ove~ hea:vy metal pollution in ~h~ coastal are~sd t human activities and the magnification of such metals within the aquaticf~~d ~hain. Copper (Cu) is one of the most ~idely detected contaminants. ~n the. e ecosystems. As microalgae are the pnmary producers and are sensitive to .mann d bi I . I I he toxi .. onmental changes, they can be use as 10 ogica too s to assess t e toxicrtyenvtr . Th . f hi d hf h avy metals in the marine ecosystems. e aim 0 t IS stu y was to assess t eo .city of Cu in the marine microalgae Dunaliella tertiolecta and Pavlova gyrans.toXICI II . bili d reacti . (ROS)The influence of Cu on growth, ce via I It~ ~n reactive oxygen species.production was [nvestigated. The 96h toxrcity test was conducted using Cuentrations rangmg from 1.28, 2.56, 5.12, 10.24 to 20.48 mglL based on~~~~rbance at 620 nrn (OD?20)' Cell viability was ass.e~s~d ~fter 0, 1,2,4, ?, and ~4h spectively by determinmg esterase enzyme activities 10 the cells stained Withfl~:rescein diacetate (FDA) .followed by flow ;yt?m~try an~lysis. The R~Sduction was determined usmg cell permeable 2 , 7 -dichlorodihydrofluorescein~~Zcetate (H2DCFDA) and flow cytometry .. ~ased on the 96 h toxicity testing, P.ns (EC 0= 3.986 mglL) was more sensitive to Cu than D. tertiolecta (EC50 =rS;2 mglt). In the cell viability test, the cultur~s ~ere exposed ~o Cuconcentrations at EC50 and 20.48 mglL. The cell Viability of D. tertiolectadecreased by 13.6 % and 51.6 % after exposure to 20.48 mg/L Cu for 4 and 6 hspectively, compared to the control. Pavlova gyrans showed a mark decrease inre II viability (87%) after exposure to 20.48 mglL Cu for 2 h. The cell viabilities ofthe control and EC50were very similar, ranging from 84 - 90% within the durationf the test (0-24 h). The ROS production increased in both species when treatedo ith Cu. This study showed that cell viability and ROS production could serve as:dditional endpoints to assess Cu toxicity in the two microalgae species.

Influence of copper on cell viability and reactive oxygen species (ROS) production in the marme microalgae dunoliella tertiolecta and pavlova gyranss

http://eprints.um.edu.my/14827/1/0001.pdf

Influence of copper on cell viability and reactive oxygen species (ROS) production in the marme microalgae dunoliella tertiolecta and pavlova gyranss

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