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Comparison five primer sets from different genome region of covid-1for detection of virus infection by conventional rt-pcr
Authors
B. Aflatoonian
A.A. Afshar
+3 more
M. Fazlalipour
D. Kalantar-Neyestanaki
H.R. Mollaei
Publication date
1 January 2020
Publisher
Abstract
Background and Objectives: The new beta-coronavirus, which caused Severe Acute Respiratory Coronavirus-2 Syndrome (SARS-CoV-2), a major respiratory outbreak in Wuhan, China in December 2019, is now prevalent in many countries around the world. Identifying PCR-based viruses is a well-known and relatively stable protocol. Unfortunately, the high mutation rates may lead to widespread changes in viral nucleic acid sequences, and so using specific primers for PCR can be recom-mended. In this study, we evaluated the power of a conventional RT-PCR to detect SARS-CoV-2 RNA among the five set primer sets. Materials and Methods: The five genomic regions of the Coronavirus SARS-2 virus including Nucleocapsids (N), Enve-lope (E), RNA depended RNA Polymerase (RdRp), ORF1ab and Spike (S) were selected for primer designing. A conventional RT-PCR was performed to compare sensitivity, specificity and other analytical characteristics of primers designed against two Real Time PCR commercial kits. Results: The result of the comparative analysis showed that the ORF1ab, N and RdRp primers had a sensitivity, specificity and positive predictive value higher than other primers. A significant difference in the analytical sensitivity between the studied primer sets in RT-PCR kits was observed. Conclusion: In this study, the ORF1ab, Nucleocapsid and RdRp regions have the best primers for identifying the SARS-CoV-2 RNA between different genes that have been suggested. © 2020, Tehran University of Medical Science. All rights reserved
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oai:eprints.kmu.ac.ir:33283
Last time updated on 08/04/2021
Simorgh Research Repository
See this paper in CORE
Go to the repository landing page
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oai:eprints.kmu.ac.ir:37765
Last time updated on 16/05/2022