Jacques, Philippe

Leclère, Valérie

Pupin, Maude

Weber, Tilmann

This chapter helps in the use of bioinformatics tools relevant to the discovery of new nonribosomal peptides (NRPs) produced by microorganisms. The strategy described can be applied to draft or fully assembled genome sequences. It relies on the identification of the synthetase genes and the deciphering of the domain architecture of the nonribosomal peptide synthetases (NRPSs). In the next step, candidate peptides synthesized by these NRPSs are predicted in silico, considering the specificity of incorporated monomers together with their isomery. To assess their novelty, the two-dimensional structure of the peptides can be compared with the structural patterns of all known NRPs. The presented workflow leads to an efficient and rapid screening of genomic data generated by high throughput technologies. The exploration of such sequenced genomes may lead to the discovery of new drugs (i.e., antibiotics against multi-resistant pathogens or anti-tumors)

Online Research Database In Technology

Bioinformatics Tools for the Discovery of New Nonribosomal Peptides - DTU Orbit(08/11/2017) Bioinformatics Tools for the Discovery of New Nonribosomal PeptidesThis chapter helps in the use of bioinformatics tools relevant to the discovery of new nonribosomal peptides (NRPs)produced by microorganisms. The strategy described can be applied to draft or fully assembled genome sequences. Itrelies on the identification of the synthetase genes and the deciphering of the domain architecture of the nonribosomalpeptide synthetases (NRPSs). In the next step, candidate peptides synthesized by these NRPSs are predicted in silico,considering the specificity of incorporated monomers together with their isomery. To assess their novelty, the two-dimensional structure of the peptides can be compared with the structural patterns of all known NRPs. The presentedworkflow leads to an efficient and rapid screening of genomic data generated by high throughput technologies. Theexploration of such sequenced genomes may lead to the discovery of new drugs (i.e., antibiotics against multi-resistantpathogens or anti-tumors).  General informationState: PublishedOrganisations: Novo Nordisk Foundation Center for Biosustainability, New Bioactive Compounds, Université Lille Nord deFranceAuthors: Leclère, V. (Ekstern), Weber, T. (Intern), Jacques, P. (Ekstern), Pupin, M. (Ekstern)Number of pages: 24Pages: 209-232Publication date: 2016 Host publication informationTitle of host publication: Nonribosomal Peptide and Polyketide Biosynthesis : Methods and ProtocolsVolume: 1401Publisher: SpringerISBN (Print): 978-1-4939-3373-0ISBN (Electronic): 978-1-4939-3375-4Chapter: 14 Series: Methods in Molecular BiologyISSN: 1064-3745Main Research Area: Technical/natural sciencesAntibiotic, Bioinformatics tools, Drug discovery, Genome mining, Nonribosomal, PeptideDOIs: 10.1007/978-1-4939-3375-4_14  RelationsActivities: In silico and CRISPR/Cas9-based tools for the metabolic engineering of actinomycetes Source: FindItSource-ID: 2291803604Publication: Research - peer-review › Book chapter – Annual report year: 2016 

Bioinformatics Tools for the Discovery of New Nonribosomal Peptides

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Bioinformatics Tools for the Discovery of New Nonribosomal Peptides

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