Alm, Martin

Dolatshahi-Pirouz, Alireza

Dufva, Martin

Emnéus, Jenny

Hemmingsen, Mette

Mohanty, Soumyaranjan

Thomsen, Peter

Trifol Guzman, Jon

Wolff, Anders

Soumyaranjan Mohanty

Martin Alm

Mette Hemmingsen

Alireza Dolatshahi-Pirouz

Jon Trifol

Peter Thomsen

Martin Dufva

Anders Wolff

Jenny Emnéus

Crossref

Biomacromolecules

3D Printed Silicone–Hydrogel Scaffold with Enhanced Physicochemical Properties

Scaffolds with multiple functionalities have attracted widespread attention in the field of tissue engineering due to their ability to control cell behavior through various cues, including mechanical, chemical, and electrical. Fabrication of such scaffolds from clinically approved materials is currently a huge challenge. The goal of this work was to fabricate a tissue engineering scaffold from clinically approved materials with the capability of delivering biomolecules and direct cell fate. We have used a simple 3D printing approach, that combines polymer casting with supercritical fluid technology to produce 3D interpenetrating polymer network (IPN) scaffold of silicone-poly(2-hydroxyethyl methacrylate)-co-poly(ethylene glycol) methyl ether acrylate (pHEMA-co-PEGMEA). The pHEMA-co-PEGMEA IPN materials were employed to support growth of human mesenchymal stem cells (hMSC), resulting in high cell viability and metabolic activity over a 3 weeks period. In addition, the IPN scaffolds support 3D tissue formation inside the porous scaffold with well spread cell morphology on the surface of the scaffold. As a proof of concept, sustained doxycycline (DOX) release from pHEMA-co-PEGMEA IPN was demonstrated and the biological activity of released drug from IPN was confirmed using a DOX regulated green fluorescent reporter (GFP) gene expression assay with HeLa cells. Given its unique mechanical and drug releasing characteristics, IPN scaffolds may be used for directing stem cell differentiation by releasing various chemicals from its hydrogel network

Online Research Database In Technology

3D Printed Silicone–Hydrogel Scaffold with Enhanced Physicochemical Properties - DTUOrbit (08/11/2017) 3D Printed Silicone–Hydrogel Scaffold with Enhanced Physicochemical PropertiesScaffolds with multiple functionalities have attracted widespread attention in the field of tissue engineering due to theirability to control cell behavior through various cues, including mechanical, chemical, and electrical. Fabrication of suchscaffolds from clinically approved materials is currently a huge challenge. The goal of this work was to fabricate a tissueengineering scaffold from clinically approved materials with the capability of delivering biomolecules and direct cell fate.We have used a simple 3D printing approach, that combines polymer casting with supercritical fluid technology to produce3D interpenetrating polymer network (IPN) scaffold of silicone-poly(2-hydroxyethyl methacrylate)-co-poly(ethylene glycol)methyl ether acrylate (pHEMA-co-PEGMEA). The pHEMA-co-PEGMEA IPN materials were employed to support growth ofhuman mesenchymal stem cells (hMSC), resulting in high cell viability and metabolic activity over a 3 weeks period. Inaddition, the IPN scaffolds support 3D tissue formation inside the porous scaffold with well spread cell morphology on thesurface of the scaffold. As a proof of concept, sustained doxycycline (DOX) release from pHEMA-co-PEGMEA IPN wasdemonstrated and the biological activity of released drug from IPN was confirmed using a DOX regulated greenfluorescent reporter (GFP) gene expression assay with HeLa cells. Given its unique mechanical and drug releasingcharacteristics, IPN scaffolds may be used for directing stem cell differentiation by releasing various chemicals from itshydrogel network. General informationState: PublishedOrganisations: Department of Micro- and Nanotechnology, BioLabChip, Fluidic Array Systems and Technology, Colloidsand Biological Interfaces, Department of Chemical and Biochemical Engineering, The Danish Polymer Centre,Bioanalytics, Biomodics ApSAuthors: Mohanty, S. (Intern), Alm, M. (Ekstern), Hemmingsen, M. (Intern), Dolatshahi-Pirouz, A. (Intern), Trifol Guzman,J. (Intern), Thomsen, P. (Ekstern), Dufva, M. (Intern), Wolff, A. (Intern), Emnéus, J. (Intern)Number of pages: 9Pages: 1321-1329Publication date: 2016Main Research Area: Technical/natural sciences Publication informationJournal: BiomacromoleculesVolume: 17Issue number: 4ISSN (Print): 1525-7797Ratings: BFI (2017): BFI-level 2 Web of Science (2017): Indexed yes BFI (2016): BFI-level 2 Scopus rating (2016): CiteScore 5.74 SJR 1.973 SNIP 1.334 Web of Science (2016): Indexed yes BFI (2015): BFI-level 2 Scopus rating (2015): SJR 2.134 SNIP 1.449 CiteScore 6.05 Web of Science (2015): Indexed yes BFI (2014): BFI-level 2 Scopus rating (2014): SJR 2.207 SNIP 1.652 CiteScore 6.38 Web of Science (2014): Indexed yes BFI (2013): BFI-level 1 Scopus rating (2013): SJR 2.085 SNIP 1.617 CiteScore 6.07 ISI indexed (2013): ISI indexed yes Web of Science (2013): Indexed yes BFI (2012): BFI-level 1 Scopus rating (2012): SJR 2.317 SNIP 1.677 CiteScore 5.72 ISI indexed (2012): ISI indexed yes Web of Science (2012): Indexed yes BFI (2011): BFI-level 1 Scopus rating (2011): SJR 2.213 SNIP 1.777 CiteScore 5.74 ISI indexed (2011): ISI indexed yes Web of Science (2011): Indexed yes BFI (2010): BFI-level 1 Scopus rating (2010): SJR 2.333 SNIP 1.66 Web of Science (2010): Indexed yes BFI (2009): BFI-level 1 Scopus rating (2009): SJR 2.288 SNIP 1.6 Web of Science (2009): Indexed yes BFI (2008): BFI-level 1 Scopus rating (2008): SJR 2.228 SNIP 1.487 Web of Science (2008): Indexed yes Scopus rating (2007): SJR 2.173 SNIP 1.528 Web of Science (2007): Indexed yes Scopus rating (2006): SJR 1.854 SNIP 1.492 Scopus rating (2005): SJR 1.643 SNIP 1.467 Web of Science (2005): Indexed yes Scopus rating (2004): SJR 1.454 SNIP 1.34 Web of Science (2004): Indexed yes Scopus rating (2003): SJR 1.17 SNIP 1.187 Scopus rating (2002): SJR 1.033 SNIP 1.148 Web of Science (2002): Indexed yes Scopus rating (2001): SJR 0.787 SNIP 0.804 Web of Science (2000): Indexed yes Original language: EnglishDOIs: 10.1021/acs.biomac.5b01722 Publication: Research - peer-review › Journal article – Annual report year: 2016 

http://orbit.dtu.dk/en/publications/3d-printed-siliconehydrogel-scaffold-with-enhanced-physicochemical-properties(7210e792-88a4-4f6f-bb17-fd4bdd0acfbb).pdf?nofollow=true&rendering=long

3D Printed Silicone–Hydrogel Scaffold with Enhanced Physicochemical Properties

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Online Research Database In Technology