Andersen, Henrik Rasmus

Hwang, Yuhoon

Jakobsen, Mogens Havsteen

Mines, Paul D.

Salatas, Apostolos

Even though nanoscale zero valent iron (nZVI) has been intensively studied for the treatment of a plethora of pollutants through reductive reaction, a quantification of nZVIreactivity has not been standardized. Here, we developed series of colorimetric assays for determining reductive activity of nZVI and its composite with granular activated carbon(GAC). The assay focused on analysis of reaction products rather than its mother compounds, which gives more accurate quantification of reductive activity. The colorimetric assays were developed to quantify three reaction products, ammonia, phenol, and aniline, generated as results of reduction of nitrate, halophenols, and nitrobenzene, respectively. The color reactions are simple and versatile since same types of reagents are able to be applied for all reactions. The colorimetric assays were further miniaturizedand optimized into 96-well microplate having 230 μL of sample volume and 2 h of reaction time. The three groups of compounds, nitrate, nitrobenzene, and para-positionedhalogenated phenols, showed graduated reactivity and were possible to distinguish a reaction mechanism between normal reduction and catalytic behaviour of second metal.The applicability was successfully proven by determining reactivity of GAC/Fe(0) composite prepared in various reduction conditions. It was shown that reactivity of GAC/Fe(0) was significantly influenced by reduction conditions, i.e. pH and reduction time, and addition of second metal further increased its reactivity. The preliminary results of GAC/Fe(0) reactivity obtained by suggested assay would be useful to determine suitable reaction condition for remediation work and estimate efficiency and required time.Therefore, suggested reactivity test with different compound combined with multiwell microplate based color assay will be useful and simple tool in various nZVI relatedresearch topics, e.g. different stabilization, immobilization, etc

Online Research Database In Technology

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Downloaded from orbit.dtu.dk on: Nov 08, 2017Microtiter plate based colorimetric assay for characterization of dehalogenationactivity of GAC/Fe0 compositeHwang, Yuhoon; Salatas, Apostolos; Mines, Paul D.; Jakobsen, Mogens Havsteen; Andersen, HenrikRasmusPublished in:The International Chemical Congress of Pacific Basin Societies 2015Publication date:2015Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Hwang, Y., Salatas, A., Mines, P. D., Jakobsen, M. H., & Andersen, H. R. (2015). Microtiter plate basedcolorimetric assay for characterization of dehalogenation activity of GAC/Fe0 composite. In The InternationalChemical Congress of Pacific Basin Societies 2015: Abstract book Honolulu, Hawaii.HawaiiUSACanadaJapanChinaAustraliaNew Zealandw w w . p a c i f i c h e m . o r g2015 International  Chemical Congress of  Pacific Basin SocietiesD E C E M B E R  1 5 – 2 0 ,  2 0 1 5  •  H O N O L U L U ,  H A W A I I8:35 – 816. 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Tawﬁk, m. elias** Principle AuthorPhotographing of presentationsand/or taping of talks isprohibited unless permission isobtained from the symposiaorganizers and individualpresenters. Final Paciﬁchem2015 programonline at:http://paciﬁchem.org/onlineprogram06 – ENVR TECHNICAL PROGRAM212–TECH    Microtiter plate based colorimetric assay for characterization of dehalogenation activity of GAC/Fe0 composite   Y. Hwang1,2, A. Salatas1, P.D. Mines1, M.H. Jakobsen3, and H.R. Andersen1  1 Department of Environmental Engineering, Technical University of Denmark, Miljøvej, B113, DK-2800 Kgs. Lyngby, Denmark 2 Department of Environmental Engineering, Seoul National University of Science and Technology, 232 Gongneung-ro, Nowon-gu, 01811 Seoul, Republic of Korea 2 Department of Micro- and Nanotechnology, Technical University of Denmark, Ø rsteds Plads, B 345E, DK-2800 Kgs. Lyngby, Denmark E-mail: yuoh@env.dtu.dk   ABSTRACT  Even though nanoscale zero valent iron (nZVI) has been intensively studied for the treatment of a plethora of pollutants through reductive reaction, a quantification of nZVI reactivity has not been standardized. Here, we developed series of colorimetric assays for determining reductive activity of nZVI and its composite with granular activated carbon (GAC). The assay focused on analysis of reaction products rather than its mother compounds, which gives more accurate quantification of reductive activity. The colorimetric assays were developed to quantify three reaction products, ammonia, phenol, and aniline, generated as results of reduction of nitrate, halophenols, and nitrobenzene, respectively. The color reactions are simple and versatile since same types of reagents are able to be applied for all reactions. The colorimetric assays were further miniaturized and optimized into 96-well microplate having 230 μL of sample volume and 2 h of reaction time. The three groups of compounds, nitrate, nitrobenzene, and para-positioned halogenated phenols, showed graduated reactivity and were possible to distinguish a reaction mechanism between normal reduction and catalytic behaviour of second metal. The applicability was successfully proven by determining reactivity of GAC/Fe(0) composite prepared in various reduction conditions. It was shown that reactivity of GAC/Fe(0) was significantly influenced by reduction conditions, i.e. pH and reduction time, and addition of second metal further increased its reactivity. The preliminary results of GAC/Fe(0) reactivity obtained by suggested assay would be useful to determine suitable reaction condition for remediation work and estimate efficiency and required time. Therefore, suggested reactivity test with different compound combined with multiwell microplate based color assay will be useful and simple tool in various nZVI related research topics, e.g. different stabilization, immobilization, etc.   

Microtiter plate based colorimetric assay for characterization of dehalogenation activity of GAC/Fe0 composite

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Microtiter plate based colorimetric assay for characterization of dehalogenation activity of GAC/Fe0 composite

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