Bentzon-Tilia, Mikkel

D'Alvise, Paul

Gram, Lone

Grotkjær, Torben

Bacterial diseases are a major constraint in aquaculture, especially in larviculture. Antibiotics that can control pathogens should be avoided due to risk of antibiotic resistance. We have shown in axenic systems of live larval feed that marine Roseobacter clade bacteria can antagonize fish pathogens and improve survival of fish larvae. Both pathogens and probionts are likely affected by the natural microbiota, and the purpose of this study was to determine if the probionts would be effective in non-axenic systems. The growth and interaction of pathogen (Vibrio anguillarum) and probionts (Phaeobacter inhibens) were studied in an Artemia and a Dunaliella tertiolecta challenge setup, and a controlled microbiota of four bacteria isolated from aquaculture was added. P. inhibens grew well in Artemia and D. tertiolecta cultures, also with a background microbiota. V. anguillarum was decreased markedly (up to four log units) by P. inhibens irrespective in presence of background microbiota. In aquaculture, the live feed is a well-known potential entry and propagation point for the fish pathogens and, hence adding the probiont at this stage would be a logical stage of introduction. This study demonstrates that probiotic bacteria can be introduced at the stage of live feed and have a pathogen reducing effect in both an Artemia and a D. tertiolecta challenge setup. This can potentially limit the subsequent use of antibiotics for control of pathogenic bacteria

Online Research Database In Technology

Phaeobacter inhibens as Probiotic Bacteria in Non-Axenic Artemia and Algae Cultures -DTU Orbit (08/11/2017) Phaeobacter inhibens as Probiotic Bacteria in Non-Axenic Artemia and Algae CulturesBacterial diseases are a major constraint in aquaculture, especially in larviculture. Antibiotics that can control pathogensshould be avoided due to risk of antibiotic resistance. We have shown in axenic systems of live larval feed that marineRoseobacter clade bacteria can antagonize fish pathogens and improve survival of fish larvae. Both pathogens andprobionts are likely affected by the natural microbiota, and the purpose of this study was to determine if the probiontswould be effective in non-axenic systems. The growth and interaction of pathogen (Vibrio anguillarum) and probionts(Phaeobacter inhibens) were studied in an Artemia and a Dunaliella tertiolecta challenge setup, and a controlledmicrobiota of four bacteria isolated from aquaculture was added. P. inhibens grew well in Artemia and D. tertiolectacultures, also with a background microbiota. V. anguillarum was decreased markedly (up to four log units) by P. inhibensirrespective in presence of background microbiota. In aquaculture, the live feed is a well-known potential entry andpropagation point for the fish pathogens and, hence adding the probiont at this stage would be a logical stage ofintroduction. This study demonstrates that probiotic bacteria can be introduced at the stage of live feed and have apathogen reducing effect in both an Artemia and a D. tertiolecta challenge setup. This can potentially limit the subsequentuse of antibiotics for control of pathogenic bacteria. General informationState: PublishedOrganisations: Department of Systems Biology, Bacterial Ecophysiology and Biotechnology, University of HohenheimAuthors: Grotkjær, T. (Intern), D'Alvise, P. (Ekstern), Bentzon-Tilia, M. (Intern), Gram, L. (Intern)Pages: 74-74Publication date: 2015 Host publication informationTitle of host publication: The Danish Microbiological Society Annual Congress 2015 : Programme & AbstractsMain Research Area: Technical/natural sciencesConference: The Danish Microbiological Society Annual Congress 2015, Copenhagen, Denmark, 09/11/2015 - 09/11/2015Source: PublicationPreSubmissionSource-ID: 118574927Publication: Research - peer-review › Conference abstract in proceedings – Annual report year: 2015 

Phaeobacter inhibens as Probiotic Bacteria in Non-Axenic Artemia and Algae Cultures

http://orbit.dtu.dk/en/publications/phaeobacter-inhibens-as-probiotic-bacteria-in-nonaxenic-artemia-and-algae-cultures(a48f9439-9908-430f-945e-96907f6c1994).pdf?nofollow=true&rendering=long

Phaeobacter inhibens as Probiotic Bacteria in Non-Axenic Artemia and Algae Cultures

Abstract

Similar works

Full text

Available Versions

Online Research Database In Technology