Baig, A.

de Vries, S. P. W.

Grant, A. J.

Gupta, S.

Hald, Birthe

Jensen, Annette Nygaard

Maskell, D. J.

Pedersen, H. S.

Wolanska, D. P.

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Nov 08, 2017Exploration of Campylobacter jejuni survival mechanisms in house fliesJensen, Annette Nygaard; de Vries, S. P. W.; Gupta, S.; Baig, A.; Pedersen, H. S.; Wolanska, D. P.;Maskell, D. J.; Hald, Birthe; Grant, A. J.Published in:Proceedings of the 18th International workshop on Campylobacter, Helicobacter &amp; Related Organisms -CHRO 2015Publication date:2015Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Jensen, A. N., de Vries, S. P. W., Gupta, S., Baig, A., Pedersen, H. S., Wolanska, D. P., ... Grant, A. J. (2015).Exploration of Campylobacter jejuni survival mechanisms in house flies. In Proceedings of the 18th Internationalworkshop on Campylobacter, Helicobacter & Related Organisms - CHRO 2015: Delegate Handbook (pp. 50-50).[0040] New Zealand.Exploration of Campylobacter jejuni survival mechanisms in house flies  Jensen, A.N.1, de Vries, S.P.W.2, Gupta, S.2, Baig, A.2, Pedersen, H.S.3, Wolanska, D.P.2, Maskell, D.J.2, Hald, B.1, Grant, A.J.2 1National Food Institute, Technical University of Denmark, Denmark 2Department of Veterinary Medicine, University of Cambridge, United Kingdom, 3Department of Agroecology - Entomology and Plant Pathology, Aarhus University  Background Houseflies have been shown to play an important role in the transmission of Campylobacter jejuni to poultry in poultry houses. An experimental fly model has been established to investigate the survival dynamics of C. jejuni in the housefly.   Objectives Identification of C. jejuni genes required for persistence/survival in the housefly.  Methods A mariner transposon mutant library of ~10,000 unique transposon insertion mutants was constructed in C. jejuni M1. Five groups of 10 flies were individually inoculated with 106 CFU of the mutant library in a 1 µl volume via their proboscis. After 4 h incubation at 20°C mutants were recovered from flies on mCCDA-chloramphenicol plates. To identify the mutants that were unable or less able to survive in flies, the relative abundance of each mutant (inoculum versus recovered) was determined by massively parallel sequencing of transposon insertion sites (Tn-seq). Directed gene deletion mutants were constructed for validation using an overlap PCR method.   Results After correction for genes required for in vitro growth of C. jejuni M1, a total of 48 genes were identified for which mutants showed >2-fold attenuated (Padjusted < 0.05) survival in flies. A number of gene deletion mutants have been generated for validation of the Tn-seq data and for further characterization.   Conclusions Using a combination of high-density transposon mutagenesis and genome-wide targeted sequencing of transposon insertion sites, we have identified a set of genes which may be required for survival/persistence in the house fly, and which could potentially serve as novel targets for intervention strategies.   

Exploration of Campylobacter jejuni survival mechanisms in house flies

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Exploration of Campylobacter jejuni survival mechanisms in house flies

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