Andersen, Stinus K.

Manns, Dirk

Meyer, Anne S.

Saake, Bodo

Journal of Applied Phycology

Dirk Manns

Stinus K. Andersen

Bodo Saake

Anne S. Meyer

Crossref

Brown seaweed processing: enzymatic saccharification of Laminaria digitata requires no pre-treatment

This study assesses the effect of different milling pre-treatments on enzymatic glucose release from the brown seaweed Laminaria digitata having high glucan (laminarin) content. Wet refiner milling, using rotating disc distances of 0.1–2 mm, generated populations of differently sized pieces of lamina having decreasing average surface area (100–0.1 mm2) with increased milling severity. Higher milling severity (lower rotating disc distance) also induced higher spontaneous carbohydrate solubilization from the material. Due to the seaweed material consisting of flat blades, the milling did not increase the overall surface area of the seaweed material, and size diminution of the laminas by milling did not improve the enzymatic glucose release. Milling was thus not required for enzymatic saccharification because all available glucose was released even from unmilled material. Treatment with a mixture of alginate lyase and a cellulase preparation (Cellic®CTec2) on large-sized milled material released all available glucose within 8 h. Application of the cellulase preparation alone released only half of the available glucose. The alginate lyase catalysis apparently induced selective removal of alginate to improve the cellulase catalyzed degradation of laminarin and cellulose in the material

Online Research Database In Technology

Brown seaweed processing: enzymatic saccharification of Laminaria digitata requires nopre-treatment - DTU Orbit (08/11/2017) Brown seaweed processing: enzymatic saccharification of Laminaria digitata requires no pre-treatmentThis study assesses the effect of different milling pre-treatments on enzymatic glucose release from the brown seaweedLaminaria digitata having high glucan (laminarin) content. Wet refiner milling, using rotating disc distances of 0.1–2 mm,generated populations of differently sized pieces of lamina having decreasing average surface area (100–0.1 mm2) withincreased milling severity. Higher milling severity (lower rotating disc distance) also induced higher spontaneouscarbohydrate solubilization from the material. Due to the seaweed material consisting of flat blades, the milling did notincrease the overall surface area of the seaweed material, and size diminution of the laminas by milling did not improvethe enzymatic glucose release. Milling was thus not required for enzymatic saccharification because all available glucosewas released even from unmilled material. Treatment with a mixture of alginate lyase and a cellulase preparation(Cellic®CTec2) on large-sized milled material released all available glucose within 8 h. Application of the cellulasepreparation alone released only half of the available glucose. The alginate lyase catalysis apparently induced selectiveremoval of alginate to improve the cellulase catalyzed degradation of laminarin and cellulose in the material. General informationState: PublishedOrganisations: Department of Chemical and Biochemical Engineering, Center for BioProcess Engineering, University ofHamburgAuthors: Manns, D. (Intern), Andersen, S. K. (Intern), Saake, B. (Ekstern), Meyer, A. S. (Intern)Number of pages: 8Pages: 1287-1294Publication date: 2016Main Research Area: Technical/natural sciences Publication informationJournal: Journal of Applied PhycologyVolume: 28Issue number: 2ISSN (Print): 0921-8971Ratings: BFI (2017): BFI-level 1 Web of Science (2017): Indexed yes BFI (2016): BFI-level 1 Scopus rating (2016): CiteScore 2.46 Web of Science (2016): Indexed yes BFI (2015): BFI-level 1 Scopus rating (2015): CiteScore 2.32 Web of Science (2015): Indexed yes BFI (2014): BFI-level 1 Scopus rating (2014): CiteScore 2.88 Web of Science (2014): Indexed yes BFI (2013): BFI-level 1 Scopus rating (2013): CiteScore 2.78 ISI indexed (2013): ISI indexed yes Web of Science (2013): Indexed yes BFI (2012): BFI-level 1 Scopus rating (2012): CiteScore 2.68 ISI indexed (2012): ISI indexed yes Web of Science (2012): Indexed yes BFI (2011): BFI-level 1 Scopus rating (2011): CiteScore 2.29 ISI indexed (2011): ISI indexed yes Web of Science (2011): Indexed yes BFI (2010): BFI-level 1 Web of Science (2010): Indexed yes BFI (2009): BFI-level 1 BFI (2008): BFI-level 1 Web of Science (2008): Indexed yes Web of Science (2007): Indexed yes Web of Science (2003): Indexed yes Web of Science (2002): Indexed yes Web of Science (2001): Indexed yes Original language: EnglishAlginate lyase, Brown seaweed, Enzymatic glucose release, Milling, PhaeophyceaeDOIs: 10.1007/s10811-015-0663-9 Source: FindItSource-ID: 2281101597Publication: Research - peer-review › Journal article – Annual report year: 2015 

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Brown seaweed processing: enzymatic saccharification of Laminaria digitata requires no pre-treatment

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