Amador Hierro, Cristina Isabel

Jelsbak, Lars

Introduction: In previous studies from our laboratory, a Pseudomonas aeruginosa lineage, named DK2, has been identified and characterized as highly successful, transmissible and persistent over four decades in cystic fibrosis (CF) patients. This lineage underwent substantial phenotypic and genetic changes over time and therefore provides a unique opportunity to explore the impact of those adaptational pathways on its ability to interact with other pathogenic bacteria such as Staphylococcus aureus, a pathogen frequently co-infecting the CF airways. Methods: We have used a novel method to study interspecies interactions between a CF isolate (2003) from the DK2 lineage and a wild-type S. aureus JE2. We grew both strains in mono or co-culture on LB agar, harvested RNA from the colonies after a 24-hour period. Subsequently we performed RNA-seq for the different samples. The data were then compared in a pairwise mode to isolate the transcriptomic profiles for each species. The most differentially expressed genes from both species were validated using real-time quantitative PCR. Results: Interestingly, the greatest expression change was observed in S. aureus, where large clusters of genes associated with virulence were differentially expressed, compared with the monoculture condition, while the P. aeruginosa DK2 response was much more discrete with isolated genes differentially regulated rather than whole operons or clusters. Conclusions: According to our data, S. aureus would display reduced virulence in the presence of an adapted P. aeruginosa DK2 clone, possibly as a consequence of the multiple hostile forces DK2 encountered over time during its long-term adaptation to the CF airways

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Rnaseq As A Method To Study Microbial Interactions Arising In The Cystic Fibrosis Airways- DTU Orbit (08/11/2017) Rnaseq As A Method To Study Microbial Interactions Arising In The Cystic Fibrosis AirwaysIntroduction: In previous studies from our laboratory, a Pseudomonas aeruginosa lineage, named DK2, has been identifiedand characterized as highly successful, transmissible and persistent over four decades in cystic fibrosis (CF) patients. Thislineage underwent substantial phenotypic and genetic changes over time and therefore provides a unique opportunity toexplore the impact of those adaptational pathways on its ability to interact with other pathogenic bacteria such asStaphylococcus aureus, a pathogen frequently co-infecting the CF airways. Methods: We have used a novel method tostudy interspecies interactions between a CF isolate (2003) from the DK2 lineage and a wild-type S. aureus JE2. We grewboth strains in mono or co-culture on LB agar, harvested RNA from the colonies after a 24-hour period. Subsequently weperformed RNA-seq for the different samples. The data were then compared in a pairwise mode to isolate thetranscriptomic profiles for each species. The most differentially expressed genes from both species were validated usingreal-time quantitative PCR. Results: Interestingly, the greatest expression change was observed in S. aureus, where largeclusters of genes associated with virulence were differentially expressed, compared with the monoculture condition, whilethe P. aeruginosa DK2 response was much more discrete with isolated genes differentially regulated rather than wholeoperons or clusters. Conclusions: According to our data, S. aureus would display reduced virulence in the presence of anadapted P. aeruginosa DK2 clone, possibly as a consequence of the multiple hostile forces DK2 encountered over timeduring its long-term adaptation to the CF airways. General informationState: PublishedOrganisations: Department of Systems Biology, Infection MicrobiologyAuthors: Amador Hierro, C. I. (Intern), Jelsbak, L. (Intern)Pages: 68-68Publication date: 2015 Host publication informationTitle of host publication: The Danish Microbiological Society Annual Congress 2015 : Programme & AbstractsPlace of publication: CopenhagenMain Research Area: Technical/natural sciencesConference: The Danish Microbiological Society Annual Congress 2015, Copenhagen, Denmark, 09/11/2015 - 09/11/2015Publication: Research - peer-review › Conference abstract in proceedings – Annual report year: 2015 

Rnaseq As A Method To Study Microbial Interactions Arising In The Cystic Fibrosis Airways

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Rnaseq As A Method To Study Microbial Interactions Arising In The Cystic Fibrosis Airways

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