Aagaard, C.

Andersen, P.

Jungersen, Gregers

Mikkelsen, H.

Thakur, Aneesh

A new (FET11) recombinant vaccine against paratuberculosis was developed based on recombinant antigens from acute and latent stages of Mycobacterium avium subsp. paratuberculosis (Map) infection.In two experiments 28 calves and 15 goats were orally inoculated with live Map in their third week of life and post-exposure vaccinated at different times after inoculation or with different vaccine constructs. In contrast to common whole-cells vaccination, the FET11 vaccine did not interfere with tests for paratuberculosis or bovine tuberculosis as no measurable antibody responses by ID Screen® ELISA, PPDj-specific IFN-γ responses or positive PPDa or PPDb skin tests developed in vaccinees. Antibodies and cell-mediated immune responses were developed against FET11 antigens, however. At necropsy 8 or 12 months of age, relative Map burden was determined in a number of gut tissues by quantitative IS900 PCR and revealed significantly reduced levels of Map and reduced histopathology. Diagnostic tests for antibody responses and cell-mediated immune responses, used as surrogates of infection, corroborated the observed vaccine efficacy: Five of seven non‐vaccinated calves seroconverted in ID Screen® ELISA at 32 to 40 weeks p.i. indicating the progression of infection, while only four of 14 FET11 vaccinated calves seroconverted at 40-52 weeks p.i. Similarly, PPDj-induced IFN‐γ responses increased over time in non-vaccinated calves, while FET11 vaccinated calves had significantly reduced PPDj IFN-γ assay responses from 40 to 52 weeks compared to non-vaccinated calves. These results indicate the FET11 vaccine can be used to accelerate eradication of paratuberculosis while surveillance or test-and-manage control programs for tuberculosis and Johne’s disease remain in place. Funded by EMIDA ERA-NET (219235), PoC East Denmark and Danish Research Council (FTP 274-08-0166)<br/

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                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Nov 08, 2017A Recombinant Multi-Stage Vaccine against Paratuberculosis Significantly ReducesBacterial Level in Tissues without Interference in DiagnosticsJungersen, Gregers; Thakur, Aneesh; Aagaard, C.; Mikkelsen, H.; Andersen, P.Publication date:2015Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Jungersen, G., Thakur, A., Aagaard, C., Mikkelsen, H., & Andersen, P. (2015). A Recombinant Multi-StageVaccine against Paratuberculosis Significantly Reduces Bacterial Level in Tissues without Interference inDiagnostics. Abstract from Immunity to Veterinary Pathogens 2015, Keystone, United States.A recombinant multi-stage vaccine against paratuberculosis significantly reduces bacterial level in tissues without interference in diagnostics   G Jungersen, A Thakur, C Aagaard, H Mikkelsen, P Andersen  National Veterinary Institute, Technical University of Denmark, and Statens Serum Institute, Copenhagen, Denmark  A new (FET11) recombinant vaccine against paratuberculosis was developed based on recombinant antigens from acute and latent stages of Mycobacterium avium subsp. paratuberculosis (Map) infection.  In two experiments 28 calves and 15 goats were orally inoculated with live Map in their third week of life and post-exposure vaccinated at different times after inoculation or with different vaccine constructs. In contrast to common whole-cells vaccination, the FET11 vaccine did not interfere with tests for paratuberculosis or bovine tuberculosis as no measurable antibody responses by ID Screen® ELISA, PPDj-specific IFN-γ responses or positive PPDa or PPDb skin tests developed in vaccinees. Antibodies and cell-mediated immune responses were developed against FET11 antigens, however. At necropsy 8 or 12 months of age, relative Map burden was determined in a number of gut tissues by quantitative IS900 PCR and revealed significantly reduced levels of Map and reduced histopathology. Diagnostic tests for antibody responses and cell-mediated immune responses, used as surrogates of infection, corroborated the observed vaccine efficacy: Five of seven non‐vaccinated calves seroconverted in ID Screen® ELISA at 32 to 40 weeks p.i. indicating the progression of infection, while only four of 14 FET11 vaccinated calves seroconverted at 40-52 weeks p.i. Similarly, PPDj-induced IFN‐γ responses increased over time in non-vaccinated calves, while FET11 vaccinated calves had significantly reduced PPDj IFN-γ assay responses from 40 to 52 weeks compared to non-vaccinated calves. These results indicate the FET11 vaccine can be used to accelerate eradication of paratuberculosis while surveillance or test-and-manage control programs for tuberculosis and Johne’s disease remain in place.  Funded by EMIDA ERA-NET (219235), PoC East Denmark and Danish Research Council (FTP 274-08-0166)  

A Recombinant Multi-Stage Vaccine against Paratuberculosis Significantly Reduces Bacterial Level in Tissues without Interference in Diagnostics

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A Recombinant Multi-Stage Vaccine against Paratuberculosis Significantly Reduces Bacterial Level in Tissues without Interference in Diagnostics

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