Aerntshorts, M.

Aerts, David

Frisvad, Jens Christian

Haeur, E.

Nielsen, Kristian Fog

Phippen, Christopher

Ram, Arthur F. J.

Teertstra, W.R.

Wosten, H.

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Nov 08, 2017FumR of Aspergillus niger is involved in production of fumonisin and secretedproteinsAerts, David; Haeur, E.; Aerntshorts, M.; Teertstra, W.R.; Phippen, Christopher; Nielsen, Kristian Fog;Ram, Arthur F. J.; Frisvad, Jens Christian; Wosten, H.Published in:Book of abstracts from the 13th European Conference on Fungal GeneticsPublication date:2016Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Aerts, D., Haeur, E., Aerntshorts, M., Teertstra, W. R., Phippen, C., Nielsen, K. F., ... Wosten, H. (2016). FumRof Aspergillus niger is involved in production of fumonisin and secreted proteins. In Book of abstracts from the13th European Conference on Fungal Genetics (pp. 380-380). [CS4T46]13th European Conference on Fungal Genetics, 3-6 April, 2016, Paris, France, Scientific Program   380  POSTER SESSION ABSTRACTS CS4T46  Tuesday 5th April  14:00 - 16:00   AERTS David (1), HAEUR E (1), AERNTSHORTS M (2). TEERTSTRA WR (1), PHIPPEN C (3), NIELSEN KF (3), RAM AFJ (2), FRISVAD JC (3), WOSTEN HAB (1)  (1) Microbiology, Department of Biology, Utrecht University, Utrecht, The Netherlands   (2) Department of Molecular Microbiology and Biotechnology, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands   (3) Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark  FumR of Aspergillus niger is involved in production of fumonisin and secreted proteins  The sporulation pathway of Aspergillus niger represses protein secretion. Colonies of this filamentous fungus secrete proteins throughout the colony except for the sporulating zone. Inactivation of the sporulation gene flbA results in colonies that are unable to reproduce asexually and that secrete proteins throughout the mycelium. In addition, the ∆flbA strain mutant strain shows cell lysis and has thinner cell walls. This pleiotropic phenotype is associated with differential expression of 38 transcription factor genes. Here, one of these regulatory genes, fumR, was inactivated. Whole genome expression analysis revealed that 8 out of 63 downregulated genes in ∆fumR are implicated in amino acid metabolism. In addition, 11 out of 15 genes of the fumonisin biosynthetic gene cluster were strongly downregulated in ∆fumR. This was accompanied by absence of fumonisin production in the deletion strain. When grown dispersed in liquid shaken cultures with xylose as a carbon source, the fumR deletion mutant showed reduced protein secretion and a different secretion profile when compared to the wild-type. This phenotype was complemented by adding amino acids to the medium. Taken together, it is concluded that fumR is involved in fumonisin production and amino acid production, the latter facilitating protein secretion. As such, fumR is an interesting lead for improving A. niger as a cell factory.     

FumR of Aspergillus niger is involved in production of fumonisin and secreted proteins

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FumR of Aspergillus niger is involved in production of fumonisin and secreted proteins

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