Bahl, Martin Iain

Fonvig, Cilius Esmann

Hansen, Torben

Hellgren, Lars

Holm, Jens-Christian

Licht, Tine Rask

Pedersen, Oluf

Roager, Henrik Munch

Zhang, Li

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Dec 21, 2017Obesity-associated fecal microbiota from human modulates body mass andmetabolites in miceZhang, Li; Bahl, Martin Iain; Hellgren, Lars; Roager, Henrik Munch; Fonvig, Cilius Esmann; Holm, Jens-Christian ; Hansen, Torben; Pedersen, Oluf; Licht, Tine RaskPublication date:2015Document VersionPeer reviewed versionLink back to DTU OrbitCitation (APA):Zhang, L., Bahl, M. I., Hellgren, L., Roager, H. M., Fonvig, C. E., Holm, J-C., ... Licht, T. R. (2015). Obesity-associated fecal microbiota from human modulates body mass and metabolites in mice. Abstract from EMBLConference 2015, Heidelberg, Germany.  Obesity-associated fecal microbiota from human modulates body mass and metabolites in mice Li Zhang1, Martin Iain Bahl1, Lars I Hellgren2, Henrik Munch Roager1, Cilius Esmann Fonvig3, Jens-Christian Holm3, Torben Hansen4, Oluf Pedersen4, Tine Rask Licht1  1National Food Institute, Technical University of Denmark, DK 2860-Søborg, Denmark 2Department of Systems Biology, Technical University of Denmark, DK 2800-Kgs. Lyngby, Denmark 3The Children’s Obesity Clinic, Department of Pediatrics, Holbæk University Hospital, DK-4300 Holbæk, Denmark 4Novo Nordisk Foundation Center for Basic Metabolic Research, Section of Metabolic Genetics, Faculty of Health Sciences, University of Copenhagen, DK-2100 Copenhagen, Denmark  Key words: Obesity, fecal transplantation, obese/control juvenile, germ-free mice, metabolites  Obesity has become a worldwide epidemic, and a possible role of gut microbiota in its etiology has been uncovered. Fecal microbiota transplantation from humans to mice allows identification of specific microbiota configurations that are causally related to obesity. However, microbial communities vary considerably among individuals, causing a possible limitation in previous works based on a few human donors. The aim of this study was to investigate the transmissibility of metabolic phenotypes with fecal microbiota using a considerably higher number of human donors. We transplanted the microbiotas from 16 obese juveniles (6-18 years old, BMI z-score median 2.84 range 1.84-3.98) and 16 controls (7-18 years old, BMI z-score median 0.095 range -0.58-0.88) separately into 64 germ-free male Swiss Webster mice caged in pairs and fed standard chow. Average body weight gain of mice with microbiotas from obese donors started to be significantly higher than the control group 7 days post colonization (dpc), ending with a difference of 8.6±4.3% of initial body weight at 51 dpc (p=0.02). Total feeding efficiency was also higher in mice with “obese microbiota”. Cecal concentrations of butyric acid and iso-butyric acid were significantly lower in the obese group, showing an altered microbial activity. No differences in serum leptin, serum lipids (triglycerides, total cholesterol, free fatty acids) or glucose homeostasis (HOMA-IR, HbA1c) were detected.  These results establish the gut microbiota as a causal factor for altered weight gain and changes in cecal levels of short chain fatty acids. This is promising given size of the donor groups, thus accounting for individual variations in the microbiota. 16S rRNA gene sequencing and qPCR of butyrate-related genes/bacteria are ongoing to reveal the structural and functional microbiota configurations as well as their dynamics; serum bile acids will be analysed to further understand the effect of microbial metabolites on obesity development. 

Obesity-associated fecal microbiota from human modulates body mass and metabolites in mice

http://orbit.dtu.dk/ws/files/111797933/Abstract_for_EMBL_conference_Obesity_associated_fecal_microbiota_from_human_modulates_body_mass_and_metabolites_in_mice_Li_Zhang_V6.pdf

Obesity-associated fecal microbiota from human modulates body mass and metabolites in mice

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