Enemark, Heidi

Gottstein, Bruno

Knapp, Jenny

Millon, Laurence

Saarma, Urma

Umhang, Gerald

Wahlström, Helene

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Dec 20, 2017Echinococcus multilocularis genotyping by the microsatellite EMSB, state of the artKnapp, Jenny; Umhang, Gerald; Wahlström, Helene; Enemark, Heidi; Saarma, Urma; Gottstein, Bruno;Millon, LaurencePublication date:2015Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Knapp, J., Umhang, G., Wahlström, H., Enemark, H., Saarma, U., Gottstein, B., & Millon, L. (2015).Echinococcus multilocularis genotyping by the microsatellite EMSB, state of the art. Abstract from 6thConference of the Scandinavian-Baltic Society for Parasitology (CSBSP6), Uppsala, Sweden.ECHINOCOCCUS MULTILOCULARIS GENOTYPING BY THE MICROSATELLITE EMSB, STATE OF THE ART  Jenny Knapp1, Gérald Umhang, Helene Wahlström, Heidi Enemark, Urma Saarma, Bruno Gottstein, Laurence Millon   1Laboratory Chrono-environnement UMR/CNRS 4962, University of Franche-Comté, Besançon, France; jknapp@univ-fcomte.fr   Introduction  Echinococcus multilocularis is a common tapeworm in foxes and rodents in the northern hemisphere and the agent of Alveolar Echinococcosis (AE). Epidemiological discontinuities are known, highly endemic in China, in expansion in Europe and rarely in North America. In this context, genetic analyses were performed to identify the parasite circulation amongst hosts in the environment at different spatial scales. A highly discriminant molecular marker was thus designed.   Materials and Methods From the first description to the assessment of its discrimination power amongst isolates from different geographical scales, 10 years of research have been performed on E. multilocularis and are summarized here.  Results The microsatellite EmsB was recently confirmed as a tandemly repeated sequence of about 250bp present in about 40 copies in the parasite genome. Its original profile hinted high power of discrimination. First, regional E. multilocularis profiles were described by the analysis of EmsB in Europe, America and Asia. In Europe, the marker has permitted to distinguish a historical area and newly endemic areas. Moreover an Arctic origin was described for the parasite recently described in Svalbard. In order to better understand its transmission pathway to human, a genotyping project on European AE has been started. From the first results, a contamination in the close environment of the patient was suspected.   Conclusion For future researches, an international EmsB database will be implemented in the EWET project (“EmsB Website for Echinococcus Typing”) to permit to researchers and physicians to better understand the source of contamination for humans and animals and achieved a targeted struggle against the parasite.      

Echinococcus multilocularis genotyping by the microsatellite EMSB, state of the art

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Echinococcus multilocularis genotyping by the microsatellite EMSB, state of the art

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