Gundersen, Maria T.

Lloyd, Richard C

Woodley, John

A step wise decision matrix is presented to quickly evaluate w - transaminase for a ‘simple scale up’ in the synthetic direction . Here a ‘simple scale up’ is defined as a system without specialized equipment or process development, thus a rapid implementation . The three step method consists of : 1 . thermodynamic evaluation, 2 . biocatalyst screen and 3 . inhibition characterization . E ach step of the method has a cut off value for easy implementation . Demonstrated by a case study which eliminated reaction pair candidates based on the cut off criteria . Finally, t he most promising candidate was intensified

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Selections of minimal conditions for a simple intensification and scale up of w-transaminase reactions

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Selections of minimal conditions for a simple intensification and scale up of w-transaminase reactions

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