Blanco, J. L.

Diaz, C. Rodriguez

Drigo, I.

Indra, A.

Janezic, S.

Jiraskova, A.

Kokotovic, Branko

Ocepek, M.

Perreten, V.

Poxton, I. R.

Rupnik, M.

Seyboldt, C.

Songer, J. G.

Weese, J. S.

Zidaric, V.

Clostridium difficile is an anaerobic sporogenic bacterium traditionally associated with human nosocomial infections, and animals have been recognized as an important potential reservoir for human infections (Rodriguez-Palacios et al., 2013). Ribotype 078 is often reported in animals but according to recent studies the overlap between PCR ribotypes found in humans and animals seems to be increasing (Bakker et al., 2010; Gould and Limbago, 2010; Janezic et al., 2012; Keel et al., 2007; Koene et al., 2011). However, genetic diversity among animal strains remains poorly understood. The aim of our work was to establish an international C. difficile animal collection with one PCR ribotype per species per country/laboratory and to compare PCR ribotypes across animal hosts and countries

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Downloaded from orbit.dtu.dk on: Dec 20, 2017Clostridium difficile PCR Ribotypes from Different Animal Hosts and DifferentGeographic RegionsZidaric, V.; Janezic, S.; Indra, A.; Kokotovic, Branko; Blanco, J. L. ; Seyboldt, C.; Diaz, C. Rodriguez;Poxton, I. R.; Perreten, V.; Drigo, I.; Jiraskova, A.; Ocepek, M.; Weese, J. S.; Songer, J. G.; Rupnik, M.Publication date:2013Link back to DTU OrbitCitation (APA):Zidaric, V., Janezic, S., Indra, A., Kokotovic, B., Blanco, J. L., Seyboldt, C., ... Rupnik, M. (2013). Clostridiumdifficile PCR Ribotypes from Different Animal Hosts and Different Geographic Regions. Poster sessionpresented at 5th Congress of European Microbiologists, Leipzig, Germany. All 112 collected strains were distributed into 39 different PCR ribotypes (Table 1, Figure 1). Up to 20 different PCR ribotypes could be found within a single animal species and up to 16 different PCR ribotypes per country. PCR ribotypes 078, 126, 014/020, 012 and 002 that are frequently associated with animals    ( Keel et al., 2007; Janezic et al., 2012) represent 40.0 % of all strains in the collection (Figure 1).  With capillary electrophoresis based PCR ribotyping subtypes were found for PCR ribotypes 001 (001 and 001ecdc), 002 (203 and 209), 014/020 (014/0, 014/5, 020, 449 and 659), SLO 036 (050 and AI-84), 078 (078 and 251), 045 (045 and 598) and 126 (126 and 078ecdc). Eight strains are nontoxigenic while toxigenic strains account for 92.9% and belong to 11              different toxinotypes: 0, I, III, IV, V, VI, VIII, XIa, XIb, XII and XIX.   V. Zidaric1, S. Janezic1,16, B. Pardon2, A. Indra3, B. Kokotovic4, J.L. Blanco5, C. Seyboldt6, C. Rodriguez Diaz7, I.R. Poxton8, V. Perreten9, I. Drigo10, A. Jiraskova11, M. Ocepek12, J.S. Weese13, J.G. Songer14, M. Rupnik1, 15,16 C. difficile  strains:  Altogether 112 strains from 12 different countries were contributed (Figure 1). Collected strains originate from 13 different animal species, including pets, horses wild animals and food animals. Approximately half (55,4 %) of the strains are from cattle and pigs (Table 1).    Clostridium difficile is an anaerobic sporogenic bacterium traditionally associated with human nosocomial infections, and animals have been recognized as an important potential reservoir for human infections (Rodriguez-Palacios et al., 2013).  Ribotype 078 is often reported in animals but according to recent studies the overlap between PCR ribotypes found in humans and animals seems to be increasing (Bakker et al., 2010; Gould and Limbago, 2010; Janezic et al., 2012; Keel et al., 2007; Koene et al., 2011). However, genetic diversity among animal strains remains poorly understood.  The aim of our work was to establish an international C. difficile animal collection with one PCR ribotype per species per country/laboratory and to compare PCR ribotypes across animal hosts and countries.  Host PCR ribotype Cattle Horses Pigs Poultry Cats and dogs Others* 001       2/2 2/1   002 2/2   2/2   1/1 1/1 003           1/1 005 1/1   1/1 1/1     010 1/1     1/1 2/2 1/1 012 2/2   1/1 1/1 1/1 1/1 015 1/1   1/1       017           1/1 018       1/1     023       1/1     027 1/1 1/1         029 1/1     1/1     033 3/3 1/1         045 1/1   2/2 1/1   1/1 056     1/1   1/1   078 5/4 2/2 8/7     1/1 081 1/1   2/2       103 1/1     1/1   1/1 126 2/2 1/1 1/1       150     4/4       413     1/1       011/049     1/1       014/020 3/3 1/1 2/1 1/1 4/2 2/2 SLO 002         1/1   SLO 012     1/1       SLO 024         2/1   SLO 036 1/1   1/1       SLO 060 1/1 1/1       1/1 SLO 066         1/1   SLO 084           1/1 SLO 084           1/1 SLO 090 1/1         2/1 SLO 125 1/1           SLO 132           1/1 SLO 133     1/1       SLO 137     1/1       SLO 143 1/1           SLO 164 1/1           SLO 165           1/1 SLO 166           1/1 Total (Nisolates=112) 31/7 7/4 31/10 11/2 15/4 17/3 Clostridium difficile PCR RIBOTYPES FROM DIFFERENT ANIMAL HOSTS AND DIFFERENT GEOGRAPHIC REGIONS Results and Discussion Materials and methods  References  1Institute for Public Health Maribor, Maribor, Slovenia; 2Ghent University, Faculty of Veterinary Medicine, Merelbeke, Belgium; 3AGES, Vienna, Austria; 4Technical University of Denmark, National Veterinary Institute, Copenhagen, Denmark; 5Complutense University, Madrid, Spain; 6Friedrich-Loeffler Institute, Jena ,Germany; 7University of Liege, Faculty of Veterinary Medicine, Liege, Belgium; 8University of Edinburgh, Edinburgh, UK; 9University of Bern, Institute of Veterinary Bacteriology, Bern, Switzerland; 10IZSVe, Treviso, Italy; 11Charles University in Prague, 1st Faculty of Medicine, Prague, Czech Republic; 12University of Ljubljana, Veterinary Faculty, Ljubljana, Slovenia; 13University of Guelph, Ontario Veterinary College, Ontario, Canada; 14Iowa State University, Ames, U.S.A.; 15University of Maribor, Medical Faculty, Maribor, Slovenia; 16Centre of excellence for integrated approaches in chemistry and biology of proteins, Ljubljana, Slovenia   Background   Molecular characterization of C. difficile strains:  All collected strains were characterized by toxinotyping (Rupnik et al., 1998; http://www.mf.uni-mb.si/tox/). In adition, binary toxin genes were detected by PCR as described in Stubbs et al. (2000). Standard agarose gel-based PCR ribotyping was used as described by Bidet et al. (1999) and results analyzed by BioNumerics software 5.10 (Applied Maths). Strains were also typed by capillary gel electrophoresis-based ribotyping using primers for standard agarose gel-based PCR ribotyping with flourescein labelled 16S primer (Indra et al., 2008). PCR ribotype patterns were analyzed and identified on a web-based database Webribo (http://webribo.ages.at).   Table 1. Overview of C. difficile PCR ribotypes found in different animal hosts  (number of isolates/number of countries)   Bakker D, Corver J, Harmanus C, Goorhuis A, Keessen EC, Fawley WN, Wilcox MH, Kuijper EJ. 2010. Relatedness of human and animal Clostridium difficile PCR ribotype 078 isolates determined on the basis of Multilocus Variable-Number Tandem-Repeat Analysis and tetracycline resistance. J. Clin. Microbiol. 48(10):3744–3749. Bidet  P,  Barbut  F,  Lalande  V,  Burghoffer  B,  Petit  JC. 1999. Development  of  a  new  PCR-ribotyping  method  for  Clostridium  difficile  based  on  ribosomal  RNA  gene  sequencing. FEMS  Microbiol. Lett. 175:261-266.  Gould LH, Limbago B. 2010. Clostridium difficile in food and domestic animals: a new foodborne pathogen? Clin Infect Dis. 51(5):577-582.        Indra A, Huhulescu S, Schneeweis M, Hasenberger P, Kernbichler S, Fiedler A, Wewalka G, Allerberger F, Kuijper EJ. 2008. Characterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping. J. Med. Microbiol. 57: 1377-1382.  Janezic S, Ocepek M, Zidaric V, Rupnik M. 2012. Clostridium difficile genotypes other than ribotype 078 that are prevalent among human, animal and environmental isolates. BMC Microbiol. 12:48 doi:10.1186/1471-2180-12-48       Keel K, Brazier JS, Post KW, Weese S, Songer JG. 2007. Prevalence of PCR ribotypes among Clostridium difficile isolates from pigs, calves, and other species. J.Clin. Microbiol. 45(6):1963-1964.       Koene MG, Mevius D, Wagenaar JA, Harmanus C, Hensgens MP, Meetsma AM, Putirulan FF, van Bergen MA, Kuijper EJ. 2011. Clostridium difficile in Dutch animals: their presence, characteristics and similarities with human isolates. Clin. Microbiol. Infect. doi:10.1111/j.1469-0691.2011.03651.x Rodriguez-Palacios A, Borgmann S, Kline TR, Lejeune JT. 2013.  Clostridium difficile in foods and animals: history and measures to reduce exposure.   Anim. Health Res. Rev 14(1):11-29.       Rupnik M, Avesani V, Janc M, Von Eichel-Streiber C, Delmée M. 1998. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36(8):2240-2247.       Stubbs  S,  Rupnik  M,  Gibert  M,  Brazier  J,  Duerden  B,  Popoff  M. 2000. Production  of  actin-specific  ADP-ribosyltransferase  (binary  toxin)  by  strains  of Clostridium  difficile.  FEMS  Microbiol. Lett. 186:307-312.       Others* : raccoons, wild hare, rabbits, goats, partridges, a goose and a crow.   001 (3,6 %) 002 (5,3 %) 005 (2,7 %) 010 (4,5 %) 012 (5,3 %) 033 (3,6 %) 045 (4,5 %) 078 (14,3 %) 081 (2,7 %) 103 (2,7 %) 126 (3,6 %) 150 (3,6 %) 014/020 (11,6 %) SLO 060 (2,7 %) SLO 090 (2,7 %) other Figure 1. Distribution of animal C. difficile PCR ribotypes from participating countries.  Pie charts show proportion of PCR ribotypes (the most prevalent in the collection) per country. The number in the centre of pie charts is the number of isolates received from the country.  The  proportions in parentheses show number of  isolates of specific PCR ribotype out of all collected isolates.  12 5 Canada U.S.A. 

Clostridium difficile PCR Ribotypes from Different Animal Hosts and Different Geographic Regions

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Clostridium difficile PCR Ribotypes from Different Animal Hosts and Different Geographic Regions

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