Canali, Chiara

Dufva, Martin

Emnéus, Jenny

Heiskanen, Arto

Martinsen, Ørjan G.

Muhammad, Haseena Bashir

Wolff, Anders

Online Research Database In Technology

                       General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.  • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal   If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.     Downloaded from orbit.dtu.dk on: Dec 20, 2017A multiplexed electrical impedance-based device for bioengineering applicationsCanali, Chiara; Martinsen, Ørjan G.; Larsen, Layla Bashir; Heiskanen, Arto; Dufva, Martin; Wolff, Anders;Emnéus, JennyPublished in:Proceedings of the 24th Anniversary World Congress on BiosensorsPublication date:2014Link back to DTU OrbitCitation (APA):Canali, C., Martinsen, Ø. G., Muhammad, H. B., Heiskanen, A., Dufva, M., Wolff, A., & Emnéus, J. (2014). Amultiplexed electrical impedance-based device for bioengineering applications. In Proceedings of the 24thAnniversary World Congress on BiosensorsA multiplexed electrical impedance-based device for bioengineering applications  Chiara Canali1, Ørjan G. Martinsen2,3, Haseena Bashir Muhammad1, Arto Heiskanen1, Martin Dufva1, Anders Wolff1, Jenny Emnéus1 1 Department of Micro- and Nano-technology, Technical University of Denmark, Kgs Lyngby, Denmark 2 Department of Physics, University of Oslo, Oslo, Norway  3 Department of Biomedical and Clinical Engineering, Oslo University Hospital, Oslo Norway chca@nanotech.dtu.dk   Electrical impedance is one of the most promising technologies used as an indicator for drug response and toxicity testing in cell-based assays, and for monitoring of cellular processes in tissue engineering applications. Bioimpedance-based medical devices have been applied for monitoring the state of tissues and the functioning of organs in vivo and in vitro.  In the present work the development of a novel, modular, impedance-based biomedical sensor is presented which is designed both for real-time monitoring of cellular dynamics within a 3D porous polymeric scaffold and for ex vivo analysis of biological tissues.  The system includes an array of four intelligent bioreactors (Fig. 1a) for parallel analysis where geometrical parameters such as electrode geometry and positioning were optimized by means of finite element (FE) simulations and mathematical analysis. An array of eight needle electrodes are positioned vertically around the periphery of the sample under study. Impedance measurements are carried out using a multiplexed approach where switching between different configurations of current-carrying (CC) and pick-up (PU) electrodes provides a greater positive sensitivity field to the volume of the sample under test (Fig. 1b).   Preliminary results obtained for assessing a 3D culture of cells (HepG2) encapsulated in a gelatin scaffold are shown in Fig. 1c. The growth of cells with time is represented by an increase in the impedance cell index. Variations between different configurations of CC and PU electrodes indicate the variation in spatial distribution of cells within the 3D scaffold. Thus, the bioimpedance-based device presented here has a high potential for real-time monitoring of the entire process of tissue engineering without affecting cell viability.      Fig 1: (a) Schematic of a single bioreactor with 8 needle electrodes, (b) FE simulation of sensitivity field using a single electrode configuration, (c) Results showing impedance CI with time for a 3D culture of cells. 

A multiplexed electrical impedance-based device for bioengineering applications

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A multiplexed electrical impedance-based device for bioengineering applications

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