Ten gravid Clarias batrachus females were administered with a single dose of common carp pituitary

homogenate (CPR). Bifore injection, the eggs were light yellow and the diameter rangedfrom 0.94 mm to

1.08 mm. Ovulation occurred approximately 12 hours after the adminstration of CPR. The colour of the

eggs turned brown and the diameter rangedfrom o. 99 mm to 1.27 mm. The fertilization rates rangedfrom

10-81%. The eggs hatched after about 30-36 hours of incubation at 26°C to 28°C. The hatching

rates ranged from 13-67070

Ang, K. J.

Cheah, S. H.

Siraj, Siti Shapor

Universiti Putra Malaysia Institutional Repository

Pertanika 13(1), 63-66 (1990)COMMUNICATION IA Preliminary Study on Induced Spawning of the CatfishClarias batrachus (Linnaeus) in MalaysiaABSTRAKSepuluh ekor induk betina Clarias batrachus yang matang telah disuntik dengan satu dos homogenatpituitari ikan lee koh. Telur didapati berwarna kuning muda sebelum suntikan hormon dan garispusatnyamenJulat antara O. 94 mm hingga 1.08 mm. Ovulasi berlaku lebih kurang 12Jam selepas suntikan kelenjartersebut. Pada masa ovulasi, warna telur bertukar menJadi coklat dan garispusatnya menJulat antara O. 99mm hingga 1.27 mm. Kadar persenyawaan menJulat antara 10-81070. Telur ikan menetas selepaspengeraman 30-36 Jam pada suhu air 26°C hingga 28°C. Kadar penetasan menJulat antara 13-67%.ABSTRACTTen gravid Clarias batrachus females were administered with a single dose of common carp pituitaryhomogenate (CPR). Bifore injection, the eggs were light yellow and the diameter rangedfrom 0.94 mm to1.08 mm. Ovulation occurred approximately 12 hours after the adminstration of CPR. The colour of theeggs turned brown and the diameter rangedfrom o. 99 mm to 1.27 mm. The fertilization rates rangedfrom10-81%. The eggs hatched after about 30-36 hours of incubation at 26°C to 28°C. The hatchingrates ranged from 13-67070.INTRODUCTIONThe freshwater catfish Clarias batrachus is animportant species for culture in Malaysia. Thoughthis fish possesses suitable culture qualities suchas resistance to low oxygen conditions and highgrowth rate, its culture in Malaysia is hamperedby fry availability. Induced spawning of C.batrachus in India using pituitary glands have beenreported by Devaraj et at. (1972) and Thakur (1978).In Thailand induced spawning of C. macrocephaluswas attempted by injecting the fish with pituitaryglands (Tongsanga et al. 1963). Though inducedspawning of C. macrocephalus using human chorio-nic gonadotropin, homoplastic pituitary extractand heteroplastic pituitary extract has beensuccessful in Malaysia (Mollah and Tan 1983;Thalathiah 1986), literature on induced spawn-ing of C. batrachus using common carp pituitaryhomogenate is not available and as such this studywas conducted. This report presents the findingsof an initial attempt at induced spawning of C.batrachus at the hatchery of the Faculty of Fisheriesand Marine Science, University PertanianMalaysia.MATERIALS AND METHODSC. batrachus broodstock were purchased from afarmer in Sabak Bernam, Selangor, Malaysia.The fish were brought back to the hatchery of theFaculty of Fisheries and Marine Science, sexedand separately stocked in two 25 ton concretetanks. The fish were fed a fish pelleted feed con-taining approximately 25070 crude protein, twicea day ad libitum and chicken liver twice a week.At the start of the study, the broodstock tankswere drained. Ten female fish that possessed apinkish genital papilla as well as a reddish ringaround the tip were selected. Ten male fish thatpossessed an enlarged pinkish papilla were alsoselected. Samples of fish eggs were taken from allthe female fish by the use of a catheter and theegg diameters of at least twenty eggs were deter-mined with an eye-piece micrometer.Twelve common carp weighing about 200 geach were killed and their pituitary glands werecollected. The glands were then homogenized withdistilled water in a tissue homogenizer. After theweights of the females and males were determin-ed, they were administered with one dose and halfCHEAH, S.H., S.S. SIRAJ AND K.J. A Ga dose of carp pituitary homogenate respectively.When the pituitary gland that was collected froma 200 g common carp was injected into a 200 gcatfish, the catfish is considered to have receivedone dose of carp pituitary homogenate.A pair of fish, was then put together in a 51 all-glass aquarium containing 2 1 of water.Approximately 12 hours after the administrationof carp pituitary homogenate, the females werechecked for their ovulatory response by applyinga gentle pressure on the abdomen. In ovulatedfish, this would result in the release of eggs from-the abdominal cavity. Unovulated fish were check-ed periodically at hourly intervals.When the female fish had ovulated, they wereremoved from the aquarium and the abdomen wasdried with a towel. Then a gentle pressure wasapplied on the abdomen from the base of the pec-toral fin towards the genital papilla. The eggs werecollected on a pre-weighed bowl to determine theweight of eggs released. A sample of at least fiftyeggs were weighed to determine the weight-number relationship to calculate the workingfecundity. The egg size of at least 20 eggs was alsodetermined with the eye-piece micrometer.The male fish were then removed from theaquarium and the ventral part of the abdomen wasslit with a scalpel. The testis were then pulled outwith pair of forceps and subsequently meshed upon a 0.4 mm sieve that was pu t on top of the bowlcontaining the eggs. Distilled water was thenused to rinse the portion of the sieve that wasretaining the testicular tissue so that the wqshingswould drop directly into the bowl. A clean chickenfeather was then used to mix the egg and testiculartissue mixture to enable egg fertilization. Themixture was then left aside for a few minutesfollowing which it was poured into an egg incuba-tion tray. The tray was partially submerged in a100 1 fibre glass tank that was connected to abiological filter.Approximately 12 h after incubation, thegastrula stage was detected in viable eggs. Suchegg samples were taken from the incubation trayfor the determination of the fertilization rate bycounting the number of viable and inviable eggs.The inviable eggs were white in colour andopaque whereas the viable eggs were translucent.About 48 hours after incubation, the hatchingrate was determined. The air stone was gradual-ly moved over the larvae to distribute them even-lyon the incubation tray. A 10 em x 10 em squarewire larvae sampler was randomly dropped intothe tray and the number of larvae that was foundwithin the sampler was counted.This was repeated twice. The number oflarvae in the tray was then estimated by extra-polating the number of larvae that was foundwithin the wire sampler to the total area of thetray.RESULTS AND DISCUSSIONThe results of the preliminary attempt at induc-ed spawning of C. batrachus is presented in Table1. As the data on two fish were incomplete, thedata on eight fish are reported. The body weightsof female fish used in this study ranged from 65g to 190 g thus indicating that C. batrachus couldmature when the body weight was only 65 g. Mostof the eggs that were taken from the fish prior toinjection were light yellow in colour. The pre-injection egg diameters ranged from 0.94 ± 0.04mm to 1.08 ± 0.13 mm in size. After the ad-ministration of common carp pituitary homo-genate, all the ten fish spawned and releasedbrown eggs. Mollah and Tan (1983) reported that880/0 of C. macrocephalus that were injected with 35mg/kg of homoplastic pituitary extract ovulatedin their study. Thalathiah (1986) reported thatadministration of 1.5-2 doses of homoplasticpituitary extract was effective to induce ovulationin C. macrocephalus.By the time of ovulation, the egg diametershad increased and ranged from 0.99 ± 0.11 mmto 1.27 ± 0.18 mm. The eggs were much smallercompared to the C. batrachus eggs in India whichhad a diameter of 1.5 mm (Thakur 1980). Withthe exception of fish number 3 where naturaloviposition had occurred and only 0.91 g of eggswere collected, the fish released between 2.75 to6.31 g of eggs. The relationship between thenumber of eggs collected and body weight ispresented in Figure 1. As the regression coefficientwas 0.30, there was poor correlation between thenumber of eggs and body weight.The fertilized eggs were demersal, adhesiveand spherical in form. The yolk was pale orgreenish yellow and contained no oil globule. Inimpregnated eggs, the blastodisc was reddish incolour. The findings were consistent with thefindings of Thakur (1980). While the fertilizationrates ranged from 10% to 81 %, the hatching rates64 PERTANlKA VOL. 13 NO.1, 1990TABLE 1Summary of data on induced spawning of Clarias batrachus with fresh common carp pituitary homogenateParameter 1 2 3 4 5 6 7 8Body weight (g) 65 100 120 120 150 160 160 190"tl Papilla colour Light Pink Light Pink Light Pink Light Pink Pink Light Pink Pink Light Pinkttl:;>;l Genital pore colour Pink Pink Pink Pink Pink Pink Light Pink Pink..,;J>Z Egg colour Yellow Yellow Yellow Yellow Yellow NA Yellow Yellow~ Egg diameter I (mm)' 0.94 1.00 0.97 1.08 1.03 NA 0.98 1.02< Std. dev. ± 0.04 ± 0.17 ± 0.10 ± 0.13 ± 0.13 NA ± 0.12 ± 0.020r Egg diameter II (mm)2 1.11 1.12 1.17 0.99 1.16 1.10 1.26 1.27;; Std. dev. ± 0.18 ± 0.09 ± 0.10 ± 0.11 ± 0.11 ± 0.15 ± 0.17 ± 0.18z9 Total egg weight (g) 2.86 4.03 0.91 6.31 2.75 4.45 5.69 3.29.:- Working Fecundity 3374- 4755 1152 74405 3483 5251 8648 4715::0<J:)Fertilization rate (0/0) 20 43 42 35 26 81 10 400Total number offertilized eggs 675 20440 484 2605 905 4253 864 1886Total number of larvae 4403 813 258 1256 609 591 247 296Hatching rate (%) 66 40 53 48 67 14 29 13Note: 1 - Preinjection size; 2 - Ovulation size, NA - Date not available.CTlU1"tl:;>;lttlt:~Z;J>:;>;l-<Vl..,c:::t:l-<ozZt:lc:::ottlt:lVl~:EzZ(Jo'TI...,:tttlo~'TIUi:te::tl5;:'"~~gS;?Zz:>ttlc:::!!3CHEAH, S.H., S.S. SIRAJ AND K.J. ANG'LOfJOo10000~OOO~6000'"'"......Q0 4000Z a~ = 2439.3395 + 18.1298x R· 0.30oaaoto thank MPKSN for supporting this projectthrough grant 50621.CHEAH, S.H.S.S. SIRAJK.J. A GDepartment of AquacullureFaculry of Fisheries and Marine ScienceUniversity Pertanian Malaysia43400 UPM Serdang, Selangor, Malaysia.Q .I--~....---...,-~--.-~---r~~~--:-!::--,~~~O 70 ~O 110 130 I~O 170 190Dody .... ight (9)g No. 0([99>Fig. 1: Relationship between number oj eggs alld body weight ojClarias batrachus.ranged from 13070 to 67070 indicating that there isa need for further research. to standardize themethodology in induced spawning of this fish.Further research should include better manage-ment of broodstock , determination of the optimumlevel of common carp pituitary to be administeredas well as refinements in the techniques employedfor fertilization and egg incubation.ACKNOWLEDGEMENTThe authors would like to thank Associate Pro-fessor Dr. Mohd. Ibrahim bin Hj. Mohamed,Dean, Faculty of Fisheries and Marine Science forthe use of the facilities and Mr. NorhananSuleiman, Mr. Azmi Yaacob and Mrs. SalimahMohd. Said for their cooperation and dedicationtowards this project. Finally the authors woulglikeREFERENCESDEVARAJ,K.V., T.J. VARGHESEandG.P. ATYA A-RAYANA RAo. 1972. Induced Breeding of theFreshwater Catfish Clarias batrachus (Linn.) by UsingPituitary Glands from Marine Catfish. Curro Sci.,41(24): 868-869.MOLLAH, M.F.A. and E.S.P. TAN. 1983. HCG-Induced Spawning of the Catfish, Clarias macro-cephalus (Gunther). Aquaculture 35: 239-247.THALATHIAH, S. 1986. Induced Spawning of Clariasmacrocephalus (Gunther). In The First Asian FisheriesForum. ed. J.L. Maclean, L.B. Dizon and L.V.Hosillos, p. 683-686. Asian Fisheries Society,Manila, Philippines.To GSA GA, S., A. SIDTHIM KA and D. MEl A-SVETA. 1963. Induced Spawning in Catfish (Clariasmacrocephalus Gunther) by Pituitary HormoneInjection. Proc. Indo-Pac. Fish. Counc. 10th. Session.p. 205-213. 10-25 October 1962. Seoul, Korea.THAK UR, .K. 1978. Investigations on the Biologyof an Air- Breathing Catfish, Clarias batrachus (Linn.)Ph.D. Thesis., University of Muzaffarpur, Bihar,India.THAKUR, N.K. 1980. Notes on the Embryonic andLarval Development of an Air-Breathing CatfishClarias batrachus (Linn.). J. Inland Fish Soc. India,12(1): 30-43.(Received 20 September, 1988)66 PERTANIKA VOL. 13 NO.1, 1990

A Preliminary Study on Induced Spawning of the Catfish

Clarias batrachus (Linnaeus) in Malaysia

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A Preliminary Study on Induced Spawning of the Catfish Clarias batrachus (Linnaeus) in Malaysia

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