The effect of various scarification treatments on the germination of seeds of Desmodium ovalifolium (Prain) was studied. Following this a pot trial was conducted to evaluate the effects of lime, phosphorus and Rhizobium inaculation on the dry matter yield, nodulation and the nitrogen and phosphorus content in the plant. Mechanical scarification by abrasion with sandpaper improved germination by 30 percent. Liming up to the highest level of 1000 kg/ha significantly increased dry matter yield and nodulation. Phosphorus increased nodule number and phosphorus content in leaves and stems. Inoculation increased dry matter yield, nodulation and the levels of nitrogen and phosphorus in the leaves and stems

Abd Halim, Mohd Ridzwan

Othman, Sariam

Universiti Putra Malaysia Institutional Repository

Pertanika 5(1), 53-57 (1982)Establishment of Desmodium ovalifolium on an UltisolRIDZWAN ABD. HALIM and SARIAM OTHMAN lDepartment of Agronomy-Horticulture, Faculty ofAgriculture, Universiti Pertanian Malaysia,Serdang, Selangor, Malaysia.Key words: Desmodium ovalifolium; Ultisol; establishment.RINGKASANKesan beberapa rawatan pelelasan terhadap percambahan biji-benih Desmodium ovalifolium (Prain)telah dikaji. Berikutan dengan itu satu percubaan pasuan telah dilakukan untuk menilai pengaruh kapur,posforas dan suntikan Rhizobium terhadap hasil bahan kering, pembintilan dan kandungan nitrogen danposforas dalam tumbuhan. Pelelasan mekanikal dengan cakaran kertas pasir meninggikan percambahandengan kadar 30 peratus. Kapur menambahkan hasil bahan kering dan pembintilan sehingga kadar kapur1000 kg/ha. Posforas meninggikan bilangan bintil dan kandungan posforas dalam daun dan batang. SuntikanRhizobium meninggikan hasil bahan kering, pembintilan dan kandungan nitrogen dan posforas dalam daundan batang.SUMMARYThe effect of various scarification treatments on the germination of seeds of Desmodium ovalifolium(Prain) was studied. Following this a pot trial was conducted to evaluate the effects of lime, phosphorusand Rhizobium inaculation on the dry matter yield, nodulation and the nitrogen and phosphorus contentin the plant. Mechanical scarification by abrasion with sandpaper improved germination by 30 percent.Liming up to the highest level of 1000 kg/ha significantly increased dry matter yield and nodulation.Phosphorus increased nodule number and phosphorus content in leaves and stems. Inoculation increaseddry matter yield, nodulation and the levels of nitrogen and phosphorus in the leaves and stems.. .INTRODUCTIONDesmodium ovalifolium, also known as D.heterocarpon, is a shrubby or procumbent peren-nial legume which grows up to two meters. It isdistributed in eastern and southeastern Asia,including Malaysia and Polynesia and also on theeast coast of Africa (Bogdan, 1977). In Malaysiait has been included in cover crop mixtures forrubber 'estates (Edgar, 1958). It is adapted todense shade, poor soils and drought conditions(Rijkebusch, 1967). D. ovalifolium has recentlyreceived attention as a pasture legume species inMalaysia. However, one of its drawbacks is itsslower rate of establishment compared to otherpasture legumes. This experiment is aimed atinvestigating some of the factors that may affectits germination, initial growth and nodulation.MATERIALS AND METHODSExperiment 1. Seed scarificationSeven scarification treatments were imposedon four replicates of Desmodium ovalifolium seeds.Each replicate consisted of 100 seeds. The treat-ments were:-To ControlT l soaked in hot water (80°C) for 5 minutesT2 soaked in hot water (80° C) for 10 minu tesT3 soaked in hot water (80° C) for 15 minu tesT4 soaked in concentrated sulphuric acid for5 minutesTs soaked in concentrated sulphuric acid for10 minutesT6 soaked in concentrated sulphuric acid for15 minutesT7 mechanical scarification by rubbing seedsbetween two boards lined willi sandpaper for about 15 seconds.After treatment llie seeds were placed incovered petri dishes lined with moist blottingpaper. They were placed in germination cabinetsset at 20°C (night) for 10 hours and 25°C (day)for 14 hours. The number of germinated seedswere recorded every alternate day for 21 days.1 Malaysian Agricultural Research and Development Institute (MARDI) Bumbong Lima, Kepala Batas, Province Wellesley,Malaysia.Key to authors' names: A.H. Ridzwan and O. Sariam.53A.H. RIDZWAN AND O. SARIAMTABLE 2Basal fertilisers other than phosphorus appliedto all treatmen15Lime was mixed into the soil medium twoweeks before sowing. Phosphorus and other basalfertilisers were applied a day prior to sowing. Theother fertilisers applied are shown in Table 2.Thinning was carried out two weeks after germina-tion to maintain a final stand of six plants per bag.Experiment II. Pot trialSeeds of D. ovalifolium were sown in blackpolythene bags of size 23 X 30 cm and 0.9 mmthick, containing 3 kg of air-dried soil. The soilwas obtained from the surface layer (0-15 cm) ofan Ultisol (Serdang sandy loam) which was formerlyunder rubber. The soil samples were analysed inthe laboratory and the chemical data are shown inTable 1.TABLE 1Chemical properties of Serdang sandy loam (TypicPaleudul t) used in the pot trial. Fertilizer kg/ha g/bag2. PHOSPHORUS Po controlPI phosphorus at 50 kg P/ha(0.73 g/bag) as TriplesuperphosphateP2 phosphorus at 150 kgP/ha (2.19 g/bag) asTriple superphosphate3. INOCULATION 10 control11 inoculated with Rhizo-bium culture forcover crops (Centrosemapubescens, Calapogoniummucunoides, Puerariaphaseoloides) obtainedfrom the Rubber ResearchInstitute of Malaysia.ppm : parts per millionThe treatments imposed consisted of factorialcombinations of three levels of lime, three levelsof phosphorus and either with or without Rhizo-bium inoculation. This gives a total of 18 treatmen tcombinations. The summary of the treatments isas follows:-1. LIME Lo controlLI 500 kg/ha (0.77 g/bag) ofground magnesium lime-stone (GML)L2 1000 kg/ha (1.54 g/bag)ofGMLRESULTS40 (K) 0.1237 (KC!)0.1 (Mo) 0.000388 (Sodiummolybdate)8 0.01238 0.0123Potassium chlorideSodium molybdateCopper sulphateZinc sulphatePhosphorus:Although phosphorus applied at the rate of50 kg/ha increased nodule number, it had noeffect on nodule dry weight. Total dry weight ofExperiment II. Pot trialLime:Liming had a positive effect on many of theparameters measured. The total plant dry weightand the dry weight of nodules of D. ovalifoliumincreased almost linearly at both levels of limeapplication (Table 3). However, a significantincrease in nodule number occurred only at thehighest lime rate of 1000 kg/ha. There was noeffect of lime on nitrogen content of the leaf butnitrogen content in the stem increased significantlyat the highest level of application (Table 4).Phosphorus levels in both the leaf and stemcomponents responded positively to limeapplication.The experiment was terminated on the 85th.day after sowing. Soil was carefully washed awayfrom the roots and the nodules were counted.After drying for 48 hours at 60° C the noduleswere weighed. The total dry weight of plants perbag was also measured.Experiment 1. ScarificationMechanical scarification by rubbing seedsbetween two sand papers gave the best germina-tion of 71.5 percent. The percentage germinationfor the control was only 39.2 percent. All othertreatments produced lower germination percen-tages than the control.5.00.12%ppm46.87 ppm28'3.5 ppm68.53 ppm9.05 ppm268.0 ppmpH (1/2.5 water)Total nitrogenAvailable phosphorusAvailable potassiumAvailable calciumAvailable magnesiumAvailable sodium54ESTABLISHMENT OF DESMODIUM oVA LIFOLIUM ON AN VLTISOL90Key:8010 controlli hot water 5 minsT2 hot water 10 nins ~70 T3 hot water 15 mins14~ 5 mins60 T5 H2SC4 10 mlnsT6 H2SO4 15 mrs50T7 rnecha1IcaI sca1flCatlon"9..-2-3G,...:..20 ~d .....2-~- ~100 To T2 T3 T4 T5 ,; l'TreatmenlSFig. 1. Mean germination percentage of Desma--dium ovalifolium seeds under differentscarification treatments (Means havingsimilar letters are not significantly differentat 5% probability level (DMRT.)D. ovalifolium increased significantly from controlwith phosphorus application of 150 kg/ha. How-ever, this was not significantly different from thedry weight obtained when phosphorus was appliedat 50 kg/ha (Table 3). Phosphorus content in bothleaf and stem increased significantly at both levelsof phosphorus .application. There was no significanteffect of phosphorus on nitrogen content in theleaf although stem nitrogen content respondedpositively (Table 4).Inoculation:Nodule number and dry weight, total dryweight of pla,nts and the content of nitrogenand phosphorus in leaf and stem were all signifi-cantly higher in inoculated treatments than inuninoculated treatments (Table 3 and 4).55Interactions:There was a significant positive teractionbetween lime and inoculation on the number ofnodules per bag. Nodule number increased withinoculation only in those treatments which receivedlime. There was no effect of inoculation on nodulenumber in unlimed treatments (Fig. 2)DISCUSSION AND CONCLUSIONThe 30 percent increase in germination ofseeds that were mechanically scarified suggests theoccurrence of hardseededness in D. ovalifolium.It is therefore desirable to practise scarificationbefore planting out under optimum conditions.The choice of the scarification method is importantsince a large percentage of seeds was damaged bythe acid and hot water treatments. The smallsized nature of the seeds may have rendered themsusceptible to damage by acid and hot water.Mechanical scarification should thus be advocated.Lime and inoculation appear to be equallyimportant in promoting the nodulation and initialgrowth of D. ovalifolium on Serdang series soil.The beneficial effects of lime in providing a suitableenvironment for the survival of indigenous as wellas introduced rhizobia especially during the earlystages of infection have been shown by Munns(1970). Apart from its neutralizing effect on soilpH, lime has been shown to encourage Rhizobiummultiplication (Anderson and Moye, 1952). Theavailability of calcium and molybdenum, bothessential for Rhizobium-legume sym biosis in acidsoils, is also improved by liming (Bould andHewitt, 1963). Liming has also been shown toreduce the concentration of some metallic ions,chiefly manganese and aluminium, which are toxicto most legumes and rhizobial strains (Dobereiner,1966). The significant interaction between limeand inoculation as shown in this experimentclearly demonstrates the positive effect of lime onRhizobium survival and vigour. From the linearresponse of plant dry matter yield to liming it islikely that liming rates above 1000 kg/ha wouldproduce even higher yields.The presence of nodules in uninoculatedtreatnYents indicates that native rhizobia whichare compatible with D. ovalifolium are presentin the soil. However, inoculation is stronglyrecommended based on the positive responsesobtained in all· the parameters measured. Theresults also show that D. ovalifolium is not highlyspecific in its Rhizobium requirement since theRhizobium culture used was not specific for thisspecies.A.H. RIDZWAN AND O. SARIAMTABLE 3Main effects of lime, phosphorus and inoculation on nodulation and total dry weight of Desmodium ova/ifoliumTreatmentsNo limeLime 500 kg/haLime 1000 kg/haNo PhosphorusPhosphorus 50 kg/haPhosphorus 150 kg/haNot InoculatedInoculatedNoduleNodule dry Total drynumber weight weight(g) (g)255 a 0.2943 a 9.4434 a298 a 0.3673 b 11.1047 b433 b 0'.5039 c 13.5285 c288 a 0.3463 a 9.9285 a357 b 0.4006 a 11.7475 ab352 b 0.4000 a 12.3978 b296 a 0.3403 a 9.0560 a369 b 0.4323 b 13.6599 bAll values are on a per bag basis.Means followed by the same letter for each factor are not significantly different at 5% level of probability (Duncan'sMultiple Range Test)TABLE 4Main effects of lime, phosphorus and inoculation on nitrogen and phosphorus content in Desmodium ovalifoliumLeaf Stem Leaf StemTreatments Nitrogen Nitrogen Phosphorus Phosphorus% % % %No lime 2.27 a 1.01 a 0.31 a 0.61 aLime 500 2.18 a LOla 0.53 b 0.78 bkg/haLime 1000 2.33 a 1.07 b 0.70 c 0.80 bkg/haNo phosphorus 2.32 a 1.00 a 0.47 a 0.69 aPhosphorus 2.20 a 1.04 ab 0.52 b 0.73 b50 kg/haPhosphorus 2.20 a 1.05 b 0.61 c 0.76 c150 kg/haNot inoculated 2.17 a 0.99 a 0.52 a 0.71 aInoculated 2.35 b 1.07 b 0.55 b 0.75 bMeans followed by the same letter for each factor are not significantly different at 5% level of probability' (Duncan'sMultiple Range Test)Responses of some pasture legumes tophosphorus application have been shown on theinland soils of Peninsular Malaysia (Kerridge andTham, 1977). Phosphorus is one of the primarylimiting nutrients in the establishment of tropicalS6pasture legumes (Norris, 1972). It has been shownto stimulate nodulation in many tropical pasturelegumes (Gates, 1974). In this experiment thebeneficial effect of phosphorus on nodulation andinitial growth is not as obvious as that of lime andESTABLISHMENT OF DESMODIUM OVALIFOLIUM ON AN ULTISOLREFERENCES7006005008. ~ooi~ 300Z'00100sooLim. kg/h.Fig. 2. Effect of lime x inoculation on nodulenumber.inoculation. Nodule number but not the noduledry weight increased with phosphorus application.This suggests that there is a greater number ofsmaller nodules when application of phosphorusincreased. The effect on dry weight of plantsbecame significant only at phosphorus applica-tion of 1 SO kg/ha. Phosphorus rate of betweenSO and I SO kg/ha would be suitable as a basalapplication for establishing D. ovalifolium underthe Serdang series soil.57ANDERSON, A.J. and MOYE, D.V. (1952): Lime andmolybdenum in clover development on acid soils.Aust. 1. Agric. Res. 3: 95-110.BOGDAN, A.V. (1977): Tropical Pasture and FodderPlants. (Tropical Agriculture Series). London.Longman Group Limited.BOULD, C. and HEWITT, E.J. (1963): Mineral nutritionof plant in soil and culture media. In: Plant Physio-logy a treatise, Vol. 1lI, (ed) F.C. Steward. NewYork and London. Academic Press.DOBEREINER, J. (1966): Manganese toxicity effects onnodulation and nitrogen fixation of beans (Phaseolusvulgaris L.) in acid soils. Plant and Soil 24: 153-166.EDGAR, A.T. (1958): Manual of Rubber Planting(Malaya). Incorporated Society of Planters. KualaLumpur.GATES, C.T. (1974): Nodule and plant development inStylosanthes humilis H.B.K.: symbiotic response tophosphorus and sulphur Aust. J. Botany 22{l):45-5.KERRIDGE, P.C. and THAM, K.C. (1977): Nutrientrequirements for establishment of legume-grasspasture on inland soils of Peninsular Malaysia. Conf."Classification and management of tropical soils,"15-20th. Aug. 1977. Kota Kinabalu, Sabah (Mimeo).MUNNS, D.N. (1970): Nodulation of Medicago sativa insolution culture V. Calcium and pH requirementduring infection. Plant and Soil. 32: 90-102.NORRIS, D.O. (1972): Leguminous plants in tropicalpastures. Tropical Grasslands 6(3): 159-170.RIJKEBUSCH, P.A.A. (1967): Notes on leguminouscover crops in sisal. Tangayika Sisal Growers Associa-tion. Sisal Res. Sta., Res. Bull. 44.(Received 4 November 1981)

Establishment of Desmodium ovalifolium on an ultisol

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Establishment of Desmodium ovalifolium on an ultisol

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