Offered have been the methods of conversion and improvement of accuracy of the capacitance and inductive transmitter measuring chains, pritected by the Authorized Certificates. The measuring chains of the capacitance and inductive transmitters, possessing the wide functional abilities and high metrological characteristics have been developed, they are protected by the Autorized Certificates. The measuring chains of the instruments and transmitters have been introduced. The introduction of the obtained results will allow to widen the functional abilities and metrological characteristics of measuring chains and the transmittersAvailable from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio

Alexander Schmidt (68515)

Erik Ahrné (1345782)

Timo Glatter (1345773)

Arbuzov, Victor Petrovich

OpenGrey Repository

THE DEVELOPMENT AND INVESTIGATION OF MEASURING CHAINS OF THE CAPACITANGE AND INDUCTANCE TRANSMITTERS

We
evaluated different in-solution and FASP-based sample preparation
strategies for absolute protein quantification. Label-free quantification
(LFQ) was employed to compare different sample preparation strategies
in the bacterium Pseudomonas aeruginosa and human embryonic kidney cells (HEK), and organismal-specific
differences in general performance and enrichment of specific protein
classes were noted. The original FASP protocol globally enriched for
most proteins in the bacterial sample, whereas the sodium deoxycholate
in-solution strategy was more efficient with HEK cells. Although detergents
were found to be highly suited for global proteome analysis, higher
intensities were obtained for high-abundant nucleic acid-associated
protein complexes, like the ribosome and histone proteins, using guanidine
hydrochloride. Importantly, we show for the first time that the observable
total proteome mass of a sample strongly depends on the sample preparation
protocol, with some protocols resulting in a significant underestimation
of protein mass due to incomplete protein extraction of biased protein
groups. Furthermore, we demonstrate that some of the observed abundance
biases can be overcome by incorporating a nuclease treatment step
or, alternatively, a correction factor for complementary sample preparation
approaches

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Comparison of
Different Sample Preparation Protocols
Reveals Lysis Buffer-Specific Extraction Biases in Gram-Negative Bacteria
and Human Cells

Timo Glatter

Erik Ahrné

Alexander Schmidt

Crossref

Journal of Proteome Research

Comparison of Different Sample Preparation Protocols Reveals Lysis Buffer-Specific Extraction Biases in Gram-Negative Bacteria and Human Cells

We evaluated different in-solution and FASP-based sample preparation strategies for absolute protein quantification. Label-free quantification (LFQ) was employed to compare different sample preparation strategies in the bacterium Pseudomonas aeruginosa and human embryonic kidney cells (HEK), and organismal-specific differences in general performance and enrichment of specific protein classes were noted. The original FASP protocol globally enriched for most proteins in the bacterial sample, whereas the sodium deoxycholate in-solution strategy was more efficient with HEK cells. Although detergents were found to be highly suited for global proteome analysis, higher intensities were obtained for high-abundant nucleic acid-associated protein complexes, like the ribosome and histone proteins, using guanidine hydrochloride. Importantly, we show for the first time that the observable total proteome mass of a sample strongly depends on the sample preparation protocol, with some protocols resulting in a significant underestimation of protein mass due to incomplete protein extraction of biased protein groups. Furthermore, we demonstrate that some of the observed abundance biases can be overcome by incorporating a nuclease treatment step or, alternatively, a correction factor for complementary sample preparation approaches

Glatter, T.

Ahrne, E.

Schmidt, A.

MPG.PuRe

https://figshare.com/articles/Comparison_of_Different_Sample_Preparation_Protocols_Reveals_Lysis_Buffer_Specific_Extraction_Biases_in_Gram_Negative_Bacteria_and_Human_Cells/2112886

THE DEVELOPMENT AND INVESTIGATION OF MEASURING CHAINS OF THE CAPACITANGE AND INDUCTANCE TRANSMITTERS

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