THE INFLUENCE OF PRE-TREATMENT AND CO-TREATMENT OF AMBON BANANA FRUIT JUICE TO FUROSEMIDE BIOAVAILABILITY

Abstract

Introduction. Some patients used banana to administered tablet or pill . It is possible that there will be pharmaceutical interaction between the drug and banana. This study was aimed to know the influence of pre-treatment and co-treatment of ambon banana fruit juice (Musa paradisiaca L.) on the furosemide bioavailability.. Method. This study was carried out using cross over design.. The subjects used in this study were five local male rabbit with same in body weight and divided into 4 treatments. The A group was treated by furosemide 80 mg (control), The B group was treated by ambon banana fruit juice 100% (5 ml) 2 hours before furosemide administration. The C and D groups were treated by ambon banana fruit juice 100% and 50% (5 ml) in the same time with furosemide administration. The blood was taken through marginalis vein of ears at the time 0,5; 0,75; 1; 1,5; 2; 3; 3,5; 4; 8; and 24 hours after furosemide administration. Furosemide in the blood was determined using Kelly’s method modified by Hakim. Then, the bioavailability parameters were measured from pharmacokinethics profiles . Furthermore, t max and Cp max were analized using one-way Anova while AUC0-8 was analized using Kruskal-wallis. Result. The average of furosemide bioavailability in male rabbit are Cpmax ; 15,21.10-2 (ìg/ml), 13,97.10-2 (ìg/ml), 11,08.10-2 (ìg/ml), 12,10.10-2 (ìg/ml), tmax; 2,30 hours, 3,50 hours, 3,35 hours , 5,30 hours, and AUC0-8 ; 10,53 ìg.ml-1 hour, 4,00 ìg.ml-1 hour, 4,95 ìg.ml-1 hour, 3,37 ìg.ml-1 hour respectively for treatment A,B,C, and D. There were no significant differences of tmax and Cpmax and AUC0-8 among groups ( p value> 0.05). Conclusion. There were no influence of pre-tretment and co-treatment of ambon banana fruit juice (Musa paradisiaca L.) on the furosemide bioavailability