Measurement of transpiration in Pinus taeda L. and Liquidambar styraciflua L. in an environmental chamber using tritiated water

Abstract

Transpiration rates of loblolly pine (Pinus taeda L.) and sweetgum (Liquidambar styraciflua L.) were measured at two different atmospheric water vapor pressure deficits (V.P.D.) in a controlled environment growth chamber using tritiated water as a tracer. The trees were maintained in a sealed plant bed containing a hydroponic nutrient solution into which labeled water (spike) was introduced. Samples of leaves, chamber air, spiked nutrient solution and control water were assayed for ratio-activity using liquid scintillation techniques to determine transpiration rates. The transpiration rate of sweetgum in ml./hr./gm. (4.95) was found to be 5 times greater than that of loblolly pine (1.03) at 1.84 V.P.D. and 8 times greater at 6.74 V.P.D. (15.99 for sweetgum vs. 2.19 for pine). Transpiration (based on measurements of leaf radioactivity) in both species rose with increasing deficit; however sweetgum increased its output by 3 times while pine only doubled its rate. Cyclical changes in transpiration rates were noted in both species; the sweetgum cycle required a 6 hour interval whereas the pine cycle required a 9 hour interval