The effect of suberythematous doses of ultraviolet and ionizing radiation on sulfhydryl groups, cholesterol and hemoglobin of the blood is studied

Yatsula, G. S.

English

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NASA TECHNICAL MEMORANDUM TM - 75106
THE PREVENTIVE ROLE OF ULTRAVIOLET RADIATION DURING
EXPOSURE TO IONIZING RADIATION
G.S. Yatsula
[ fNASA-TM-75106) THE PREVENTIVE ROLE OF H78-33710
• ULTRAVIOLET RADIATION D U R I N G EXPOSDBS TO
IONIZING RADIATION (National Aeronautics and
Space Administration) 9 p HC A02/8F A01 -^ Uaclas
( CSCL 06B &Z/52 31659
Translation of "0 profilakticheskoy roll
ul'trafioletovogo izlucheniya v sluchaye
vozdeystviya ioniziruyushchey radiatsii".
Gigiyena i Sanltarlya, No. 2,
February 1978, pp 48-52
NATIONAL AERONAUTICS AND SPACE ADMINISTRATION
WASHINGTON D.C. 20546 MAY 1978
https://ntrs.nasa.gov/search.jsp?R=19780025767 2020-03-22T01:46:28+00:00Z
THE PREVENTIVE ROLE OF ULTRAVIOLET RADIATION DURING
EXPOSURE TO IONIZING RADIATION "'
G. S. Yatsula
The author studied the effect of suberythematous doses of ultra-
violet and various doses of ionizing radiation on sulfhydryl groups,
cholesterol and hemoglobin of the blood. More favorable indices of
cholesterol and hemoglobin content of the blood were noted in those
animals that during exposure to ionizing radiation were subjected to
ultraviolet radiation.
A number of works (Ye. G^Zhuk; T. A. Sviderskaya, et al. , and others) have /48*
studied the combined action on animal organisms of ultraviolet, x-ray and y-
radiation. As a rule these studies employed single large doses of ionizing
radiation. There are no published descriptions of the peculiarities of the effect
on the organism of small and fractional doses in combination with ultraviolet
irradiations.
We conducted four series of tests on'-aQ-binorats using different doses of an
external ionizing radiation in'combination with ultraviolet. In all the series the
animals in the second, fourth and sixth groups were irradiated daily by a mercury . 1
quartz lamp PRK-4 for 2-f- min. In this time they received 10 and 25 meal of ultra-
violet radiation per 1 cm2 of body surface,, which equalled •§- erythema dose. Pre-
liminary ultraviolet irradiation of the animals in these groups was conducted in the
first series of tests for 90 days, in the second series—?8, in the third series—
^Numbers in margin indicate pagination in the original foreign text.
TABLE i
CHARACTERISTICS OF GROUPS OF ANIMALS IN TESTS WITH ULTRAVIOLET AND EXTERNAL
IONIZING RADIATION
Group of
rats
Number of erythema
doses during ultra-
violet -irradiation
for days for per-xod
of test
Ionizing irradiation
power of
exposure
dose,
R/min
total ex-
posure dose
for test
period, R
integral
absorbed . -;
tissue dose
in rads for
test period
2-a
3-H
4-H
5-H
6-H
0,5
0,5
1 Series .
1-H
2-H
3-H; : '
4-H r
5-H .
6-H ; .;.
• _'••••-
0,5;
. • ~°- •'
:
- 0,5
\
67,5
,:"• 67,5. . . ' ' ; : '
67,5, ,'
^, • • : '. - •
0,000017
,0,000017
0,000034
0,000034
. .. — -
' • 1,08. '
:V.'l,08 V;.,;-
: .2,16
2,16
:
.C:f''£:~i~'!''
'-/"-•I'.CM^iH-
:;>;u' 1,04-^r
••'••-.«.. 2,01 ->-X...-
• - . , • • 2,01. /;.'
" ' " - , - • ' ' - '
 f, ' -""• - ' .-i.- ! ' *)
• :;:0'^ '';"" • ••' ":.-:'•' •• ,, . ' n; series'^ •• •'.•/•:::;::;;:•;.;' v.:,;i^ S-.
l-H •• :
.2-H -;• A:--
3-H .
4-H
5-H
6-a :' .
•— ••..••.'••'
?:>... 0,5 ^ .;.-,
' b 5 -•
,.; 1.
- 0,5 -
. •-: ' __ - • , - - ' •
:•}'-- 107,0 -:-
'" — . ' • ..
107,0
, .'.' • ' .' '': • ''.
107,0 .
"^".T — • — *-.:..• -..-.
.'„ i-,:. -'••-.• •
0,000051 '
•0.000051
0,000068
0,000068
': '-'.V ',;.':— ~*-'l:- ••'.-•
H. .'.',.-. ,•.__ '.; -.-•-- .
. , ' . : . 9,80
9,80
13,10
13,10
• > '.r-'."^ "^ -:!;;.''
. ' .''---s-'j- •^'j<?"-7-'-'
' ; • ' ; ' - . -"9,1 1 ';•'•;>, ,
' ' ' 9,1 r •
12,18 V12,18;,.
- '. ' . ' • ' , ' • ' ' . • ' • : . ' - ' ': " •. - -V • ' • > • < - . • ' • ' • , , ' ' - , ' - ' " ' ' "
• • ' ' • ' . ' : • • ' ' • ' ' . • ' • " ' • '''•'• - ' in 'Series ' • • - : V-':'Y"- ' ' - .
1-H
2-H -
3-H
4 - H . • - . . .
5-H
6-H
—9,5
- ;' ___
0,5
-' - — .'
0,5
—• ; • • 65,o ' ,
.. •• . ' - .
65,0 .
—65,0 ,
..... — .
.-. .... __ ^ .
13.3^14,4
13,3—14,4
13,3—14,4
13,3—14,4
. ' — • •
'.".'''—••' .
.'••' 300 •/•
. 300 : ' •
' ..600 .
' 6 0 0
••-..' ' •'•
; ' — •; '; '
• —••-•;.•,'•
-••285"v:v.-r.
2 8 5 ' • •
570 y:.1/.
570 •''. • '
IV Series
64,5
64,5
64,5
—
13,3—14,4
13,3—14,4
13,3—14,4
13,3—14,4
—
750
750
900
• 900
—
712
712
855
855
80, in the fourth series—68 days. The total-duration of the ultraviolet irradi-
ations of animals, including in combination with the ionizing, was for the first
series of tests 135 days, for the second—21^ days, for the third—130 days, and
for the fourth--129 days.
After the indicated preventive ultraviolet irradiations the animals in the
third-sixth groups of all the test series began to be exposed to external ionizing
radiation. For irradiation of animals in the first and second test series pre-
parations were used that were made of uranyl nitrate which were placed in open
lead boxes and arranged at a distance of 15 cm from the animal's back. The
TABLE 2
CONTENT OF SULFHYDRYL GROUPS IN BLOOD SERUM OF'ALBINO, RATS
DURING ULTRAVIOLET AND EXTERNAL IONIZING IRRADIATION'(M±m)
Group of rats Sulfhydryl groups, pjnole per 100 mg of blood serum
I series
1-
2- .
3-
4- ' : '. :
5-
6-.
53^4,5
45±2,3 .
. 50^7,0,
61^2,7 .
48^5,1
84=tl,5 .
3 II series Illl series llV series
64i7,l
67±3,2
62±4,1 ,:
72=t4,2
64i3,l . ,
67 :^4,1
. 9,7±7.8 .
105±7,4
137±10,6
115±8,8
137^13,5
115±8,7
107^8,2
119^3,1
121:±13,0
101=£11,2
' 103±16,0
12$±:7,2
preparations were plates 10x12 cm in size on which 10 g of uranyl nitrate were
uniformly distributed. Irradiation of the animals in the first test series by
these preparations went for 24 hours a day for 45 days, of the animals in the
second series—136 days. The ultraviolet irradiation also continued for this
same period. In the third and fourth series the animals received fractional doses
of x-rays of 50-150 R for one session with the help of RUM-3 and RUM-11 apparatus.
For the test period in each series 6 sessions were conducted of x-ray irradiation
of the animals with 7-day intervals. With the help of the methods described in
special works the integral absorbed dose of ionizing radiation was computed in
rads for the entire test period.
In the first series of tests 65 male albino rats were used, in the second
series—?2, in the third series—79 and in the fourth series—72 which were distri-
buted into six groups. Data on the nature of the effect on the animals by the
studied factors are presented in table 1.
This report presents the results of an analysis of the sulfyhdryl groups,
cholesterol and hemoglobin in the animals' blood. According to the data of a
number of researchers (P. D. Gorizontov; T. A. Sviderskaya, N. N. Glushchenko, et
al.) these indices can to a sufficient degree characterize the effect of radiant
energy on the organism of humans and animals. The content of sulfhydryl groups
in the blood serum was determined by the method of amperometric titration with the
aid of 0.001 normal solution of mercuric chloride, and was expressed in micromoles
per 100 ml of serum (S. N. Nistratova, Ye. M. Kedrova). The cholesterol content
in the blood was established by the method of Engel'gardt and Smirnova, and was
expressed in milligram-percents. The amount of hemoglobin was computed by a
Sali hemoglobinometer in relative percents. The average data on the changes in
the content of sulfhydryl groups in the blood serum of animals under conditions of
ultraviolet and external ionizing irradiation are presented in table 2.
A certain increase in the sulfhydryl group content in the blood serum as
compared to the control was found in animals of the second group in the second-
fourth series of tests with ultraviolet irradiation. In animals of the third and
fifth groups of the first and second series the ionizing radiation in doses from
i;04 to 12.18 rads did not produce significant changes in the number of sulf-
hydryl groups in the blood serum as compared to the control. During ionizing
irradiation*: of animals in the third and fifth groups of the third series with dose /50
285 and 570 rads a statistically reliable increase was established for the content
of sulfhydryl groups as compared to the control. In the fourth series of tests
irradiation of the animals in the third group with dose 712 rads caused a certain
increase, while in rats of the fifth group that received 855 rads, a certain
decrease in the number of sulfhydryl groups in the blood serum as compared to the
control.
The combination of ultraviolet irradiation with ionizing in all the test
series, as a rule, resulted in a higher content of sulfhydryl groups in the
blood serum as compared to the control.
Ultraviolet irradiation of animals in the fourth and sixth groups of the first
and second series of tests who received from 1.0*4- to 12.18 rads of ionizing
radiation caused a higher content of sulfhydryl groups in the blood serum than
in animals of the third and fifth groups that received only ionizing radiation in
the indicated doses. With combined x-ray and ultraviolet irradiation of animals
in the fourth and sixth groups of the third series the content of sulfhydryl
groups in the blood serum was reduced as compared to animals in the third and
fifth groups which were only exposed to x-rays. This decrease with a dose of
285 and 5?0 rads was 16$ (D > 0.05), however the absolute number of sulfhydryl
groups in.these cases was higher than in the control, as a result of which one can
speak of the normalizing effect of ultraviolet radiation on the content of sulf-
hydryl groups during x-ray irradiation of animals. Ultraviolet irradiation of
TABLE 3
CONTENT OF CHOLESTEROL IN BLOOD (in mg£) OF ALBINO RATS
OF DIFFERENT TEST SERIES .(M±m)
Group of rats
2-
3-
4-^ . *
K.
*J
•6-
I series) II series
• 88i2,0
89=tl/2
88=t4*4
85=tf,6
59±3 4
65^2|4
56±4,9
55±3,l
55±3,9
51^2.8
III seri:es
119=t4,l
114±3,l
12l±6!5
100±4,7
124=t6,5
IV seiies
V |
10G--4!o v
82^4,0
' 101^2,0 ,
87^6,0
89^4,0 '
animals in the fourth series of tests in combination with x-rays in a dose of
712 rads (fourth group) caused a decrease in the number of sulfhydryl groups in
the blood serum by V7% (D>0.05) as compared to the animals that with the same dose
of x-ray radiation did not receive ultraviolet. However the given number did
not differ from the control, therefore one can evaluate the effect of the ultra-
violet irradiation as normalizing. Under analogous conditions (sixth group) with
a ''dose of 855 rads of x-ray radiation an increase was noted in the number of
sulfhydryl groups in the blood serum by 2$% (D > 0.05). On this basis one can
consider that the ultraviolet irradiation of animals has a favorable: effect on
the organism.
Average data on the content of cholesterol in the blood under our test
conditions are given in table J.
As compared to the control the ultraviolet irradiations of animals in the
second group of all the test series did not result in significant changes in the
cholesterol content in the blood. In the first and second series no significant
deviations were noted in this index under the influence of ultraviolet and ionizing
radiation, which can indicate the lack of an effect by the studied factors on
cholesterol exchange. In tests of the third series using ultraviolet and x-ray
radiation fluctuations in the blood cholesterol content were within 4-16% as
compared to the control. Ultraviolet irradiation of the animals in this series in
combination with x-rays in an integral absorbed dose of 285 rads (fourth group)
caused a drop in the cholesterol content by Jfo (D X).05) as compared to the
animals that with these doses of x-rays did not receive ultraviolet irradiation
(third group). However the amount of cholesterol in this case was close to the
TABLE if.
AMOUNT OF HEMOGLOBIN IN BLOOD (in %} OF. ALBINO RATS OF
DIFFERENT TEST SERIES (M±m)
Group of rats
1-
2-
3-
4-
5-j • ' ' .
6- .
* r
I series
87^0,52
91^1,30
83^1,30
90^1,30
84=tl,80
89±0,80
II series
98±1,66
lOUtl.tl
102il,10
100±0,92
102±2,10
102^1,39
III series
96:±2,50
97±0,80
90±0.70
94^1,10
85±1,70
93±1,50
IV series
91=tl,93
94±:2,25
85±:1,10
91=tl,l7
82:±0,76
90±1,40
control which indicates the normalizing effect of ultraviolet irradiation on the
cholesterol exchange. Under analogous conditions with a dose of 570 rads (sixth
group) an increase was noted in the blood cholesterol content by 2h% (D < 0.05).
Data from the fourth test series indicate that the amount of cholesterol in the
blood under the influence of the studied factors is reduced by 3~33$ as compared to
the control which is apparently related to inhibition of the liver functioning.
'_ -.Ultraviolet irradiation of animals in this series in combination with x-rays
in a dose of 712 rads promoted an increase in the amount of cholesterol in the
blood by 2Jfo (D < 0.05), and with a dose of 855 rads— only by 2.J?o (D > 0.05) as
compared to -the animals who with these doses of x-ray radiation did not receive
ultraviolet rays, Consequently, the latter improved the cholesterol-forming
function of the liver.
Average data on the amount of hemoglobin in the blood of animals under our
test conditions are given in table 4.
Changes in the content of hemoglobin in the blood of animals in different
groups of the first and second series of tests under the influence of the studied
factors fluctuated within 2-5% as compared to the control. Ultraviolet irradiation
of the animals in combination with ionizing in a dose from 1.04 to 12.18 rads had
an effect to a lesser degree on the level of hemoglobin in the blood than in animals
who with the same doses of ionizing radiation did not receive ultraviolet irradiation.
In the third and fourth test series the amount of hemoglobin in the blood of animals
of different groups was recorded as 1-15% Df the control. Ultraviolet irradiations
of the animals in these series in combination with x-rays in doses from 285 to
855 £ads promoted an increase in the amount of hemoglobin in the "blood by 5-1
(D < 0.05) as compared to the animals that .were not exposed to ultraviolet
irradiation.
The data obtained in our experiments on the amount of sulfhydryl groups,
cholesterol and hemoglobin in the blood indicate the favorable role of ultra-
violet irradiation of animals exposed to external ionizing irradiation.
REFERENCES
.. •
Gorizontov, P. D., in Patologicheskaya fiziologiya ostroy luchevoy bolezni
[Pathological Physiology of Acute Radiation Sickness], Moscow, 1958, p. 5.
Glushchenko, N. N., V. S. Danilov, and Yu. P. Kozlov, Radiobiologiva. No. 4 (197*0,
P. 591.
Zhuk, Ye. G., Gig, i san, No. 10 (1958), p. 84.
Kedrova, Ye. M., Biokhimiya. Vol. 27.(1962), p. 685.
Nistratova, S. N., in Tiolpvyye soyedineniya VL meditsine fThiol Compounds in
Medicine], Kiev, 1959, p. 7-
Sviderskaya, T. A., Ye. G. Zhuk, I. N. Filipson, Gig. _i_san., No. 2 (i960) , p. 27..
Sviderskaya, T. A., in Ul'trafioletovoye i^ .lucheniye [uitraviolat Radiation],
Part 4, Moscow, 1966, p. 174.
Copyright Holder: Gigiyena 1 Sanitariya, 19?8
STANDARD TITLE PACE
1. Report No.
.. 'NASA TM-75106
2. Government Accession No.
4. Title end Subtitle
THE PREVENTIVE ROLE OF ULTRAVIOLET RADIATION
DURING EXPOSURE TO IONIZING RADIATION
7. Author(t)
G.S. Yatsula
9. Performing 6'ganiiofien News end Addran
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Translation
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15. Svpplomentory Notei
Translation of "0 profilakticheskoy roli ul'trafioletovogo
izlucheniya v sluchaye vozdeystviya ioniziruyushchey radiatsii"
Gigiyene i Sanitariya, No. 2, February 1978, pp 48-52,
16. Absrroct
• The effect of suberythematous-doses of ultraviolet
and ionizing radiation on sulfhydryl groups,
. cholesterol and hemoglobin of the blood is
studied. ' •• - . • • '
17. Koy Word* (Selected by Autnor(i)) 18. Dinr ibui ion Stotement This .copyrighted
Soviet work is reproduced and sold
T>y NTIS under license from VAAP, the
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The preventive role of ultraviolet radiation during exposure to ionizing radiation

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