Investigation of the free flow electrophoretic process

Abstract

The effects of gravity on the free flow electrophoretic process was demonstrated. The free flow electrophoresis chamber used to demonstrate the effects of gravity on the process was of a proprietary design. This chamber was 120 cm long, 16 cm wide, and 0.15 cm thick. Flow in this chamber was in the upward direction and exited through 197 outlets at the top of the chamber. During electrophoresis a stream of sample was injected into the flow near the bottom of the chamber and an electrical field was applied across the width of the chamber. The field caused a lateral force on particles in the sample proportional to the inherent change of the particle and the electric field strength. Particle lateral velocity was then dependent on the force due to viscous drag which was proportional to particle size and particle shape dependent