The influence of sinusoidal modulated currents was studied and physical loads on the nucleic acid content and the nucleotide composition of the total RNA in muscles of rats of various ages under conditions of hypodynamia were measured. Methodology utilized is described and conclusions are presented

Sokolova, Z. A.

English

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NASA TECHNICAL MEMORANDUM
	 NASA TM-76162
THE DYNAMICS OF CERTAIN INDICATORS OF NUCLEIN METABOLISM
DURING HYPOKINESIA IN RATS OF DIFFERENT AGES UNDER THE
INFLUENCE OF SINUSOIDAL MODULATED CURRENTS AND MEASURED
PHYSICAL LOAD
(NASA-TM-76162) THE DYNAMICS OF CEdTAI N
	 N80-32058INDICATORS OF NUCLEIN METABOLISM DURING
HYPOKINESIA IN RATS OF DIF'rhk(EdT AGES UNDEb
THE INFLUENCE OF SINUSOIDAL MODULATED
	 Uucla6
CURRENTS AND (Nat.ioual Aerouautics d"d Space G5/51 28664
Translation of "Dinamika nekotorykh pokazateley nukleinovogo obmena pri
gipokinezii u krys raznogo vozrasta pod vliyaniyem sinusoidal'nykh modul-
irovannykh tokov i dozirovannoy fizicheskoy nagruzki", Voprosy Kurortologii,
i Fizioterapii i Lechebnoy Fizicheskoy Kul'tury, No. 5, Sept.-Oct. 1977,
pp 67-70
NATIONAL AERONAUTICS AND SPACE ADMINISTRATION
WASHINGTON, D. C. 	 20546	 MAY 1980
$TAND^A0 TIT LE PAGE
1. Revert No. 3. Gotremaitnt Accession Me. 1. RKiploef's Catalog No.
XASA TM-76163	 -
A. Trite and 3eiutie The Dynamics of Certain Indic*- S. Report Date
I tore of ftelsio Metabolism during Hypokinesis in ax 19RO
a. Petta rmlov Orgsnisefion CodeRats of Different Ages under the Influence of
S1nuso dtatl Modulated Currents and Measured Pbvei
g . Performing Orgenisetlen Report No.cal Load	
-.	 ^_^.-..^..^
7	
c
iJ.	 Work Unit Ho.j	 ,Z.	 A	 Sokolov*
It.
	
Contract or Brent No.
NAS w•g. Prtiarm , mg Organisation Memo and Address
13. Type of Report sail PerieJ ColoredSCiTRAN
fox 5456
RarhazA	 CIA 91108 Translation
r
F it. $' nse 09 A one Name and Address
'^1:ina3 Aerouautics and Space Administration
Washington , D. C.	 Y0546 it. Sponsoring Agency Cad.	 {
4}
'IS. itipploteent" Mates
,Translation of "Dinamika nekotorykh pokazateley nukleinovogo obmena pri
ISipokinezii u krys raznogo vozrasta pod vliyaniyem sinusoidaltnykh
^modulirovannykh tokov i dozirovannoy fizicheskoy nagruzki tt , Voprosy
Kurortologii, i Fizioterapii i Lechebnoy Fizicheskoy Kul t tury, No. 5,
Sept. - Oct. 1977, pp 67-70
14. Abstract'	 Y
This article described a study of the influence of sinusoidal Modulated
currents (SMC) and measured physical loads on the nucleic acid content
and the nucleotide composition of the total RNA in muscles of rats of
various age under conditions of hypodynamia.
Methodology utilized is described and four conclusions are presented.
i
	
17. Key Words (t:tiettt; a i6y Aiilhor(s))	 W. Oisitib0ion Statement
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:i :aC^ w.as '^CG^	 1	 ^3:IC wv	 e:	 9	 111
THE DYNAMICS OF CERTAIN INDICATORS OF NUCLEIN METABOLISM DURING
HYPOKINESIA IN RATS OF DIFFERENT AGES UNDER THE INFLUENCE OF SINUS-
OIDAL MODULATED CURRENTS AND MEASURED PHYSICAL LOAD
Z. A. Sokolova*
/67**
Hypodynamia, as a condition which is widespread under modern condi-
tions of the development of scientific-technical progress, is accompanied
by significant disruptions in the functions of various systems in the
organism. It aggravates the course of an illness during forced immobi-
lization of a patient undergoing treatment. It has been established that
hypodynamia is a factor in premature aging, accelerates in young animals
the formation of the aged mechanism for regulating physiological processes,
reduces adaptational capabilities of an organism with age (V. V. Frol'kis
and I. V. Muravov, M. R. Mogendovich, V. A. Boyer; Kraus and Raab, and
others). It may be presumed that biochemical shifts in an organism during
a period of hypokinesia, as well as during the effect of physical facto'-s,
will have a different character depending on age. In connection with this,
we undertook the problem of studying the influence of sinusoidal modulated
currents (SMC) and measured physical load on the nucleic acid content and
the nucleotide composition of the total RNA in muscles of rats of various
age under conditions of hypodynamia.
*Biochemical Laboratory (Director--Dr. of Medical Sciences I. D. Frenkel')
of the Experimental Dept. (director--Dr. of Biological Sciences 0. A.
Krylov) of the Central Institute of Health Resort Treatment and Phy-
siology, Moscow.
**Numbers in margin indicate pagination of foreign text.
1
Work was conducted with 100 male rats of the Vistar strain, which
were divided into two groups by age. In the first group were rats aged
25 - 30 days weighing 50 - 70 g, and in the second -- rats aged 55 - 60
days weighing 140 - 180 g. By the end of the experiment, the rats in
the first group were reaching two months of age, and in the second group --
three months.
Hypokinesia was produced for 30 d_-ys by placing the rats into individual
cages which limited their ability to move. Part of the rats from each
group received the influence of SMC during the time of immobilisation,
and part received measured physical load. The source of the SMC was a
"Amplipulse-3" apparatus, the carrier frequency was 5000 Hs, modulation
frequency was 100 and 30 Hz, its depth was 100% , and current intensity
was 2 - 3 mA,with 2nd type of operation. The electrodes were placed in the
area of the right and left thighs of the rat. The procedure was repeated
for 20 minutes twice a day for 20 days. Used as physical load was a
treadmill run which lasted 2 minutes daily for a period or 20 days.
The nucleic acid content was determined by the two-wave spectrophotometric
method of R. G. Tsanev and G. G• Fiarkov, and the nucleotide composition of
total RNA in the muscles -- by the method of ion-exchange chromatography
on columns with the DAUM N-50 (Katz and Comb).
The immobilization of the rats in both groups for 30 days led to a
sharp reduction in RNA content in the leg muscle, which in rats of the
first group comprised 23%, and the second -- 2,b 0
 and was statistically
accurate (P < 0.001). The DNA content, however, did not change ( table 1!.
2
Nora kinssia Hypakinesia+ Hypokinesia +
days) +SMC (100 H physical load
Rats in Group 1
63,23±3,13 48,81±1,99 59,50,,83 50,44±3.04
485±1, 8944,53± 1,97 4702127 44, 90-1. 1, 79
X0,5 X0,5 >0,5
Rats in Group 2 1
61,37±1.98 45,6,5±1,67 57,742,44 46,29+1,53
X0,001 <0.0 ]0,5
52,93±2,04 52.33±1,44 53,01±1,30 51,00±1,95
X0 1 5 >0,5 ]0,5
Ipdicator
RNA
P
IWA
RNA
P
IWA
The effect of SMC on rats of both groups during hypodyrmda hindered
the reduction of the amount of RNA in the muscle tissue. A140 9
 its content
in the muscles of experimental rats exceeded the level in rate without the
Influence of 3MC by a statistical value, and practically did not differ
from nu..imal values. SMC had no affect on 24A content in the skeletal
muscles of experimental rate (see table 1).
With measured physical load for rats of both groups found in a state
of hypokinveiap the RNA content in the skeletal muscles remained completely
reduced, as in the control animals.
The hypokinesia produced in rats of the first group was accompanied
at the same time by a change in the nucleotide composition of total RNA
In the muscle tissue in the form of a statistically accurate increase in
uridine nucleotide (table 2).
Table 1
Content of nucleic acids (in mg % P per dry -tissue weight) in skeletal
muscles of different age rats in the norm, during hypokinesia and the effect
of SMC and measured physical load (M : m)
/68
3
In studying the nucleotide content of^muscls RNA it was shown that
the development of a hypokinetic state in rats of the second group had
much in common with that of the first group -- a certain increase in
uridins nucleotide war noted.
The application of SMC with frequency modulation of 100 He to hypo-
kineticised rats in the lot group did not noticeably affect the nucleo-
tide composition of the RNA j but with frequency modulation of 30 H$ the
nucleotide composition of the total RNA in the skeletal muscles changed
noticeably -- there was a statistically certain reduction in the content
of cytidin nucleotide and an increase in the proportion of uridins nucleotide.
The value of the specificity coefficient of the total RNA decreased reliably.
The was data was obtained also during the study of the effects of
SKC of various parameters on the nucleotide composition of muscles of rats
in the 2nd group ( sse table 2).
Under the influence of metered physical loads the relation of nucleo-
tides in the total RNA of the muscle tissue did not change.
The obtained results made it possible to establish a great similarity
in the character of changes in the indicators studied in rats of different
ages during the time of developing the hypokinetic state as well as under the
influence of the applied factors. Thus # hypokinesia produced in rats of
both groups led to unidirectional changes in indicators of nuclein metabolism --
a significant reduction in RNA content and a change in the relation of
nucleotides ir.. the total RNA of the leg muscles. It is possible that these
changes are the basis for the disruption in providing muscle tissue elasticity.
We have noted the expressed influence of SMC on nucleonic metabolism
indicators in the muscle tissue of hypokinsticised rats of both groups.
4
hlectrical stimulation of muscles using SMC hindered the reduction of RNA
content in then. The specifics cf SMC influence on the nuoleotide composition
of total RNA in the muscles depending on current parameters were revealed.
Thus $ SMC with frequency modulation of 100 Hs had no expressed influence on
the nucleotide composition of the total RNA in rats of both groupsg while
with a frequency modulation of 30 Has it reliably reduced their RNA specificity
coefficient ---	 Such changes may be associated with the fact that
under the influence of SMC there is an intensification of the biosynthesis
of RNA fractions with the nucleotide compositon e which differs from the
nucleotide composition of total RNA and thereby has different functional
properties.
Table 2
	
/§2
Nucleotide opposition (in sstol. %) of total RNA in the skeletal muscles
of rats of different ages in the norms during hypokinesia, and during the
influence of SHC and measured physical load (H ± m)
Hypo- Hypo-	 Hypo-
Indicator
	
Norm kinesia:	 is kinssia rpokinesia+
00 days C liz C(3oHa +physical
Rats in Croup 1
	
low
Adenin
Guanin
Orti
U din
Rats in group 2
20,9±1,67121,6±1,37	 20.4^ 1.53
>0,5	 >b.5
32,9±2,85121,41'.32	 312	 1,55
,1 	 .l
21,1±1,31120__.2*^1.23	 21 ,^8^ 1,39
>0,25	 Do,26
25,1±1,15
	 29.9±1,70	 26,	 1.44
<0.05	 , I
1,19±0,09( 0,95±0,07 11.14±0,07 10,79±0,02 10,95±0,06
>0.05	 }0.05	 <0.05	 >015
P nucleotide (A) 1
 20.8±0,83 120.4 . 11.66	 19 12}2.00 ( 16,6±0,76  119.6 .70
>	 X0.5
P lcleotide (n	 31.5±1,54	 2970,
	}±	 30,8±b,74	 30>b±0 5,03	 31,3t 5]	 0.95^0
din mcleotide (w
	20.5±1.56	 18,	 1,11	 21.	 2,59
	
13,8±0,94	 19,9±0,95
P	 1	 0,25	 25	 CO301	 (	 ]0,25
micleotide(Y)	 27,2±1,39
	
31,111,25
	 28.1;t1.51	 39.1	 1,55	 29,2±1,391	 <0105	 ,1	 001
	 >0,25
A=-7
r+U	
1.07±0,04
	 0.96±0,05	 1.12±0,11	 0,80±0,03	 1,07±0.02
P	 >0.1	 >0, I	 <0.02
	 X0,5
enine nucleotide
P
GuanP
 - nucleotide
_Cytidin nucleotide
P
Uri
	 nomleotide
r+u
A+Y
P
16.2 ,1,06
ZS>0.5
15.3±1.16C0.02
39.6±1,61
<0,001
27,4±1,09
>0,5
21, .5 22)
28,3±2,33
>0,5
23,2t1.945
U/tlL1.^
ne ZOOR 
G?Uq<, 4- is1 }^
5
According to data found in literature, messenger RNA has a low
specificity coefficient (0. P. Samarina and oo-authors, K. G. Gasaryan
seal N. G. Shuppye, M. N. blinov.). A comparison of the obtained results with
this data allows us to suppose that after the influence of SHC with frequency
modulation of 30 Hs, conditions are created in the muscles for an in-
tensified synthesis of messenger RNA. The intensification of synthesis of
messenger RNA which occurs in the nucleus may be closely tied with the
activation of the genetic apparatus of the muscle cell under the given
experimental conditions. This hypothesis is based on the results of our
previous studies (Z. A. Sokolovs and co-authors), in which it was established
that with the influence of SMC in muscles of hypokinsticized rata, poly-
pleidisation processes were intensified, the content of dry DNA substance
for a single nucleus was increased. A comparison of the data obtained in
this work with results of our previous studies and with the literature
lead us to conclude that under the influence of SMC in hypokineticized
animals, the functional capability of the nuclear cell apparatus In increased,
and consequently also that of the muscle tissue as a whole.
Conclusions
1. Hypokinesia lasting 30 days produced in tats aged 1 and 2 months
led to a reduction in RNA content and to a change in the relation of nucleo-
tides in the total RNA of the muscle tissue.
2. The application of SMC to these rats during the period of immobiliza-
tion retarded the reduction of RNA level in the muscles.
/LO
6
3. With the IrAU*nae of SW with frequency modulation of 30 Hs,
the specificity coefficient of muscle tissue RNA was reduced, while it
remained unchanged at a frequency modulation of 100 Hs.
4. Measured physical load apW- , ' to the hypokineticised animals
had no significant effect on the contem. of nucleic acids and the nucleo-
tide composition of total RNA in the skeletal muscles.
L17SRATURE
Blinov M. N. "Biokhimiya", 1967, No. 2, p. 323.
Boyer V. A. In the books Dviptel'naya activnost' i stareniye.
("Motor Activity and Ageing"). Kiev, 1969, p. 240.
Gasaryan K. G•, Shuppye N. G. "Bickhimiya", 1966, No. 4, p. 687.
Mogendovich M. R. In the books Dvietel'naya aktivnost' i stareniye.
("Motor Activity and Ageing"). Kiev, 1969, P. 227.
Samarins 0. P., Lerman M. I •, T1mwVan V. D•, et al. "Biokhimiya",
1965, No. 4, p. 880.
SOkolova Z. A., Popov V. I., Krylov 0. A. "Vopr. kurortol", 1976,
No. 1, P . 57 •
Frol I kin V. V., Muravov I - V. In the books Fizicheakaya kul'tura --
istoohnik dolaoletin. ("Physical Culture -- The Source of Longevity").
M., 1965, p. 24.
Taanev R. G•, MarkOV G. G. "biokhi>aiya", 1960, No. 1, P. 151.
Katz J., Comb D. "J. biol. Chew.", 1963, v. 238, P. 3063.
Kraus H., Haab W. Hypokinetic Diseases Diseases Produced by Lack of
Exercise. Springfield, 1961•
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The dynamics of certain indicators of nuclein metabolism during hypokinesia in rats of different ages under the influence of sinusoidal modulated currents and measured physical load

The dynamics of certain indicators of nuclein metabolism during hypokinesia in rats of different ages under the influence of sinusoidal modulated currents and measured physical load

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