The gastric secretory function of rats was studied in 4, 8, 16 and 30 day hypokinesia. Inhibition of both the gastric juice secretory and acid producing functions was found. The greatest inhibition was observed on day 8 of limited mobility. By days 16 and 30 of the experiment, a tendency of the gastric secretory activity to return to normal was observed, although it remained reduced

Markova, O. O.

Proshcheruk, V. A.

Rozvodovskyy, V. I.

Vavryshchuk, V. I.

English

NASA Technical Reports Server

  
 
 
N O T I C E 
 
THIS DOCUMENT HAS BEEN REPRODUCED FROM 
MICROFICHE. ALTHOUGH IT IS RECOGNIZED THAT 
CERTAIN PORTIONS ARE ILLEGIBLE, IT IS BEING RELEASED 
IN THE INTEREST OF MAKING AVAILABLE AS MUCH 
INFORMATION AS POSSIBLE 
https://ntrs.nasa.gov/search.jsp?R=19800015482 2020-03-21T18:51:21+00:00Z
1,
NASA TECHNICAL MEMORANDUM
	 NASA TM-76034
INFLUENCE OF EXPERIME t
 TAL HYPOKINESIA ON
GASTRIC SECRETORY FUNCTION
0.0. Markova, A.S. Vaveyshchuk, V.I. Rozvodovs'kyy
and V.A. Proshcheruk
Translation of "Vplyv eksperymen'bal'noyi gipokineziyi na
sekretornu funktsiyu shlunka," Fiziolo^iehnyy zhurnal (Kiev),
Vol. 23, No. 5, 1977, pp. 653-656
(NASA-TM-76034) INFLUENCE OF EXPERIMENTAL
HY,POKINESIA ON GASTRIC SECRETORY FUNCTION
(National Aeronautics and Space
Administration) 8 p HC A02/M'F A01
-080-23374
Unclas
G5/52 19324
r ^ g on F9
ti
NATIONAL AERONAUTICS AND SPACE ADMINISTRATION
WASHINGTON, E.C. 20546	 APRIL 1980.
y
e, .>. _.
	 ^	 .,,....^_^,	 .,rn. n^
	 r_rxser _r'i e•..x_..	 w.v	 ..	 remu.v s^ xn e	 ^	 xa v>	 n .as w f was	 ^	 a. .w	 It
1F
t
F
i
e
STANDARD TITLE PAGE
- 71. Report No,
NASA TM-76034
2,	 Government Accession No. 3;	 Recipient's Catalog No,
4. Title and Subtitle
	 INFLUENCE OF EXPERIMENTAL S.	 Repo 'e or ' G^j 	 ] ^$0ZHYPOKINESIA ON GASTRIC SECRETORY FUNC- 6. Performing Org19 otionCodeTION
t. Author ( s )	 0.0.
	 Markova,	 A, S.	 Vaveyshchuk, B.	 Performing Orgoni action Report f<,,^
V.I.	 R.ozvodovs t ky,Y.. and. V.A.	 Proshcheruk,
10.	 Work unit No.Dept. of Patholog i c al Phys iol . , Ternopol
Medical Institute, Ternopol, iJSaR
9.	 Perlorming Organiaotton Name and Address 11.	 Contract or Grant No.
•teo.Kanner Associates N S - 1
13.
	
Type of Report and Period CoveredRedwood City, Calif orn ia 9 4063
Translation
12.	 Sponsoring Agency Name and Address'
National Aeronautics and Space Adminis-
tration, Washington, D.C. 	 20546 1,.	 Sponsorin g Agency Code
15.	 Supplementary Notes
Translation off' tt Vplyv eksperymental t noyi gipokinez yi na
sekretornu funktsiyu-shlunka, tt Fiziolohichnyy zhurnal (Kiev),
Vol.	 23,,.No.	 5,	 1977,	 pp .	 653-656
16.	 Abstract
The gastric secretory function of rats was studied in 4,	 8,
16 and 30 day hypokinesia. 	 Inhibition of both the gastric
juice secretory and acid producing functions was found. 	 The
greatest Inhibition was ohser'ved on day 8 of limited mobility.
By days 16 and 30 of the experiment, a,tendency of the gastric
secretory activity to return to normal was observed, though
it remained reduced.
17.	 Key Words (Selectod by Author(s))	 10.	 Distribution Statement
"This copyrighted Soviet work is repro-
duced and sold by NTIS under license
from VAAPr the Soviet copyright agency.
No further ` copying,is permitted without
19. SecurityClasaf. (of Ohio 	 permission	 from VAAP."
Unclassified	 Unclassified	 1	 8
3
^x
7
y
INFLUENCE OF EXPERIMENTAL HYPOKINESIA ON
GASTRIC SECRETORY FUNCTION
0.0. Markova, A.S. Vaveyshchuk, V.I. Rozvodovslkyy
and V.A. Proshoheruk
Dept. of Pathological Physiology, Ternopol
Medical Institute, Ternopol, USSR
The problem of hypokinesia is important to the extensive clinical
	 /653*
practice, since the ill usually are constrained by a bed regimen for a
certain period of time.
The question of the effect of hypokinesia on the nervous, cardio-
vascular, endocrine and locomotor systems, immunobiological protective
responses and metabolism have been dealt with extensively in the
literature. The effect of hypokinesia on the digestive system has not
yet been explained adequately.
Study of the gastric secretory and acid production funo4l;ions can
produce the effects of changes in the stomach. Explanation of these
processes can assist in making up proper diets for the ill and, also, in
the development of preventive treatment preparations directed toward the
mainenanoe of normal functions of the gastrointestinal tract in a long
term bed regimen. In recent years, there have been indications of
disturbance of the digestive system as a result of limitation of motor
activity in the literature [3, 81.
We studied the pattern of changes of the gastric secretory function
in 30 day hypokinesia.
Method
r
Five sex,iesof studies were conducted on 62 random bred white rats
of both sexes, weighing 150-220 g. The animals of control series I were
in conventional vivarium cages, and the other four series had preliminary
limitation of their motor activity for 4, 8, 16 and 30 days. To produce
Numbers in the margin indicate pagination in the foreign text.
1
phypokinesia, the rats were placed in specially constructed metal group
cages with individual sections. The cage design permitted their volume
to be changed to correspond to the size of the rat, by which the stand-
ardization of the conditions of their maintenance is increased.
The food and drink containers were placed in the front part of the
cage. The animals were fed bread and milk. The gastric juice was ob-
tain^.id in an acute study [9]. Eighteen days before the start of the
study, bread feeding of the rats was stopped, with unlimited access to
water. Then, under urethane anesthesia (250 mg/kg intraperitoneally),
the animals were fixed, back down, to a stand with rubber bands. A
cylinder was placed under the back. The hair of the upper part of the
abdomen in the epigastric region was shaved ., and a 1.5-2.0 cm incision
was made in all layers of the anterior abdominal wall. A ligature was
applied between the pylorus and the duodenum. Here, care was taken not
to injure or tie the vessels. The animals were in this condition for
2 days. Forceps then were applied to the esophagus, the stomach was
removed., and it was washed in physiological solution and dried. All the
contexts of the stomach then were removed. The amount of gastric juice
was measured in m! during the 2 day tie off. The acidity of the gastric
juice (total, free, bound) was determined microchemically [1]. The
study results were processed by the generally accepted methods of the
statistics of variation.
i
	 Results and Discussion
The study results are presented in the table, from which it is
evident that, in hypokinesia, the gastric secretion is depressed. The
volume of the gastric secretion on the fourth day of limited mobility
decreases b y 39%. On day 8, the amount of gastric secretion decreases 	 /654
further (by 55%, compared with the control). Subsequently, a tendency
of the gastric secretion to return to normal is observed, but it remained
reduced. On day 16 of the study, the amount of gastric juice secreted
was 42% below normal. On day 30 of hypokinesia, the gastric secretion
level was lower than the control by 39% (p<0.05). The acid production
function of the stomach (see figure) also was observed during hypokinesia.
We observod a 35% decrease of total acidity of the gastric juice on the
2
.,riii
fourth day of observation. The most marked decrease in total acidity was
observed on days 8 and 16 of hypokine s ia. On day 8, total acidity de-
creased by 59% and, on day 16, by 55^o below normal. Subsequently, on
day 30 of hypokinesia, some tendency towards an increase in total acidity
was observed, but it remained 30% below the control, values.
SOK
	0.	
6 16 
	 j K _ 4	 B	 !o	 JJ
Changes of gastric secretory activity in percent in
experimental hypokinesia: ordinate, average secretory
activity indices; a. amount of gastric juice; b. total
acidity; c. total hydrochloric acid; d. free and e.
bound hydrochloric acid; abscissa, day of hypokinesia;
K, control.
AVERAGE INDICES OF GASTRIC SECRETORY ACTIVITY OF RATS IN
CONTROL AT DIFFERENT TIMES OF HYPOKINESIA
ywosw AVP
Klnb eib is
wro covey, t,
,taro ae I .
y MA
C	 d	 !	 e	 J f
3arwnb m	
n1A u IIC? I nCw wiHCIni'r.: ofwwcn r"
	
13o'w^eiu t`o
g Km,Fponw
h nnogiiwln,
4 x111
P
h I'lnuxiftc3l.t,
8 A111B
P
^, rinoktuc^la,
16 n111c
P
h rinoxina3in,
30 nnln
P
83,31-3,655 29,19:0,509 03,7410,832 34,8710,301)
54,4016 , 134 25.421:2,225 48,G7:J;2,F44 23 ,25f2,098
0,001 >0,05 <0,01 <0,01
	
0,701:0 ,222	 34,54:L-1,688 12,09;4; 1,507 32,941:7,030 22,341+0,995()RrGINAL PAGB IN
<0101
	
<01001
	
;0,001
	
<0 ,001	 <0,001 OV POOR QUALITY
	
0,90:1. 0,409	 37,811.4.785
	
12,7010,858 34,431;1,876 20,241;3,(101
	
<0,05
	 <0,001
	 <0,002	 00,001
	
<0,002
	
0,951.;0,049	 51,201:4,797 15,011:I,510 3G,8513,2G2 17,$31.2,638
	
<0,05	 <01001
	
<0,001	 <01001	 00.001
1.1A±0,220
0,941;0,118
<0.05
Key: a. Study conditions	 e. Total HC1
b. Amount of gastric juice f. Bound HC1
secreted per day, ml	 g. Control
c. Total acidity	 h. Hypokinesia, 4, 8,
d. Free HC1	 16, 30 days
In prolonged hypokinesia, depression of hydrochloric acid produc-
tion also is observed. On day four of hypokinesia, there was a signif-
icant 24% decrease in total amount of hydrochloric acid, which was
3
greater than the amount of bound hydrochloric acid (p<0.01). A decrease
in concentration of free hydrochloric acid in this period was sign1fi-
cant (p{0.05)	 At later times in hypokinesia, the indices of both the
free and bound hydrochloric acid decreased significantly, which was
evidence of considerable weakening of the secretory ability of the sur-
rounding cells. Thus, after 8 days of hypokinesia, the concentration
of free hydrochloric acid decreased by 59% and that of bound, by 36%,
On days 16-30 or hypokinesia, some tendency was observed towards an
increase in concentration of free hydrochloric acid, but it was 56% and
49% below normal., respectively. The concentration of bound hydrochloric
acid at these observation times always was reduced, and it was 49% below
the control on day 30.
Thus, our studies show that, in hypokinesia, there is depression of
both the gastric secretory and acid production functions, and the great-
est depression of it was observed on day 8 of limited mobility.
It is evident from the literature that, as a result of 15 day
hypokinesia of xeople, with a single meal of preserved foodstuffs, a
45% reduction in gastric secretory activity was observed and, against the
background of this decrease, a 32% increase in the indices of bound acid-
ity and a 50% increase in free hydrochloric were observed [4]. In study
of the effect of hypokinesia, together with acceleration, on the human
body, there is a decrease in both the gastric secretory and acid produc-
tion functions [2, 4-63. There also is a decrease in the enzyme secre-
tion function of the pancreas in limitation of the motor activity of
rats [73. Apparently, changes of the secretory activity of the intestinal
glands observed by many authors are associated with both decreases in
muscle activity and with unusual diets, change in its amount and qualita-
tive composition and, besides, as a result of exposure of the human body
to such an extreme factor as acceleration. The changes we found in the
secretion of gastric juice evidently are associated with a reduction of
the influx of stimuli from the receptors of the motor analyzer to the
central nervous system. In turn, this leads to weakening of the
efferentation, which may be a cause of the depression of the gastric
secretory and acid production functions.
4
Some tendency of the secretory activity to return to normal., ob-
served after 30 day hypokinesia, can be considered a sign of adaptation
of the body to unusual living conditions.
A
5
RE FE TUEJAITCH3
1 . GorbenIto, F. G, , "MI cmc herr,icaI ' 1 ,,,-tr, rm1nat lon of total acidity and
fxoe hydrochloric acd.:>i in ,h(,, . ; to mach contents," Klinich .
meditoirla 10, 1 8-50 (1953)
2. Xegorov, L.x,, K.V. Smirnov,
	 . Korotayev and M.V. Lukasheva,
"The effect of some extrome actlona on the functional state of the
:stomach," Koomich. biol. a. meet. 2, 71-74 (1967) .
3. Kraonykh, I.G. and L.F. Tyutina, "The motor evacuation function of
they
 gastrointeotinal trac'- of the dog under conditions of p,o-
longed hypodynamia," Kosmich. biol. i med. 5, 40-44 (1973).
4. Lukasheva, M.V., "The secretory function of the stomach of healthy
people under normal conditions and in exposure to some extreme
space flight factors," author's abstract of candidate's dis-
sertation, Moscow, 1968, 14 pp.
5. Smirnov, K.V., L.S. Potemkina, L.G. Goland and A.G. Bar.akov, "The
effect of transverse accelerations on the secretory activity of
the gastrointestinal tract of the dog," Kosmich. biol. i med. 4,
29-33 (1964).
6. Smirnov, K.V., L.S. Potemkina, L.G. Goland, N.P. Goncharova, F.A.
Semenova and V.I. Lechen'kov, "The effect of space flight on the
state of the enzyme secretion function of the digestive systems
of cosmonauts," Kosmich. biol. i med. 6, 61-65 (1970).
7. Smirnov, K.V., L.G. Goland and I.L. Medkova, "The effect of limita-
tion of motor activity on the enzyme secretion function of the
pancreas and the external secretory function of the liver of the
rat." Kosmich. biol. i med. 3, 22-26 (1974).
8. Sorokin, P.A., V.V. Simonenko and B.A. Korolev, "Clinical observa-
tion of prolonged hypodynamia," Probl. kosmich. biol. 12, 24-33
(1969)'.
9. Shay, H,, S.A. Komarov, S.S. Fels, D. Meranze, M. Gruenstein and
H. Siplet, "A simple method for the uniform production of gastric
ulceration in the rat," Gastroenterology 5, 43 -61 (1945)•
Copyright: Vydavnytstvo "Naukova dumka" " Fiziolohichnyy zhurnal", 1977
ORIGTNAL P
OP POOR AG -
[JAL17
6


Influence of experimental hypokinesia on gastric secretory function

Abstract

Similar works

Full text

Available Versions

NASA Technical Reports Server