This study quantifies surface denudation of carbonate rocks by the first application of in-situ cosmogenic <sup>36</sup>Cl in China. Concentrations of natural Cl and in-situ cosmogenic <sup>36</sup>Cl in bare carbonates from Guizhou karst areas were measured with isotope dilution by accelerator mass spectrometer. The Cl concentration varied from 16 to 206 ppm. The <sup>36</sup>Cl concentrations were in range of (0.8–2.4)×106 atom g−1, resulting in total denudation rates of 20–50 mm ka−1 that averaged over a 104–105 a timescale. The <sup>36</sup>Cl-denudation rates showed roughly a negative correlation with the local mean temperature. This preliminary observation may suggest the variations of proportions of chemical weathering and physical erosion in denudation process, depending upon local climatic conditions