Retention of Lumpy Skin Disease Virus in <i>Stomoxys</i> Spp (<i>Stomoxys Calsitrans, Stomoxys Sitiens, Stomoxys Indica</i>) following intrathoracic inoculation, Diptera: Muscidae

Abstract

AbstractLumpy skin disease (LSD) is an emerging disease in cattle in Kazakhstan and the means of transmission remains uncertain. In the current study, acquisition of Lumpy Skin Disease Virus (LSDV) by Stomoxys species following intrathoracic inoculation was demonstrated under laboratory conditions. Flies were injected with a virulent LSDV strain into the thorax region to bypass the midgut barrier. The fate of pathogen in the hemolymph of the flies was further examined using PCR and Virus isolation tests. LSDV was isolated from all three Stomoxys species immediately and up to 24h post intrathoracic inoculation while virus DNA was detectable up to 7d post intrathoracic inoculation.</jats:p