PURIFICATION STUDIES ON THE INFECTIOUS CANINE HEPATITIS VIRUS : II. VIRAL PARTICLES AND THEIR CHARACTERISTICS REVEALED BY MODIFICATION OF THE PURIFICATION PROCEDURE

Abstract

The picture of the infectious canine hepatitis virus particle derived from infections tissue culture fluid and cells has been clearly demonstrated by a modified purification procedure. It was able to observe the fine structure of the particle when the virus was stained with phosphotungstic acid or sodium phosphotungstate. Fixing the purified virus particles with 2% neutral formalin resulted in the revelation of a clearly hexagonal or pentagonal image. Purified ICH virus particles keep infectivity and complement fixing and hemagglutinating activities and can be disintegrated by treatment with ether with the liberation of smaller particle, soluble antigen and hemagglutinin. The ultraviolet absorption spectrum of final purified virus particles shows a maximum at 258 mμ and a minimum at 240 mμ