A built-in three-in-one screen-printed electrode assembly containing nano bismuth species deposited silver as working, pre-oxidized silver as reference and unmodified silver as counter electrodes (designated as SPAgE-Bi(nano)), has been developed for simple electrochemical sensing of H(2)O(2) in pH 7 phosphate buffer solution. The working electrode showed similar to 250 mV reduction in over-potential and a two-fold increased peak current values for electrocatalytic reduction of H(2)O(2) over the respective unmodified silver working electrode (i.e. SPAgE) following a diffusion-controlled electron-transfer mechanism. 50 nm sized Bi particles on SPAgE surface was seen in SEM picture of the working electrode. Linear range of H(2)O(2) detection from 100 mu M to 5 mM, a sensitivity of 0.627 mu A/mM and detection limit (S/N = 3) of 56.59 mu M were obtained. Finally, SPAgE-Bi(nano) sensor assembly was utilized for cosmetic H(2)O(2) determination, where obtained values and labeled values were in good agreement with recovery in the range of 94.75-101.03 %
