尖鐮孢菌為造成萎凋之病原，可危害許多重要經濟作物。其中由 Fusarium oxysporum f. sp. cubense 引起之香蕉黃葉病乃世界香蕉栽培的主要限制因子之一，不僅在巴拿馬及中美洲各地造成嚴重的經濟損失，並在台灣肆虐蔓延導致台灣香蕉嚴重減產。快速且良善的檢測系統，可為作物病害管理的基石。計畫主持人已開發出一套快速檢測流程，能快速、簡便且可靠的進行香蕉黃葉病菌生理小種第四型之鑑別工作，經評估後可應用於田間病原菌之檢測，本計畫擬將此快速檢測技術應用於田間實務上，藉此自疑似帶菌的香蕉組織與田區土樣中快速檢測出是否為攜帶該病原菌之檢體 ，同時對全台香蕉黃葉病菌之生理小種分布作全盤的調查，祈能對香蕉黃葉病之防治工作有所助益。此技術平台之發展經驗，也將可作為開發其他重要經濟作物之鐮孢萎凋病菌的分子鑑定與檢測技術之重要參考依據。Fusarium oxysporum is one of the most important pathogens which cause wilt in many economically important crops. Fusarium wilt of banana, commonly known as Panama disease of banana, has become epidemic in Panama since 1890. The Panama disease of banana caused by F. oxysporum f. sp. cubense (Foc) (E. F. Smith) Snyder & Hansen is a potentially devastating disease throughout the world and the major limiting factor for banana production. A rapid and reliable diagnosis is the foundation of integrated disease management practices in commodity crops. We have develpoed an optimized PCR assay, the developed molecular method was rapid, simple, and sensitive, and could identify Foc race 4 specifically. We also demonstrated that Foc race 4 in naturally infected banana tissues could be easily tagged by using our PCR assay. In this research, the rapid identification technique will be applied for the detection of Foc pathogen in the field samples of infected plants and soils. This detection technique will also be used to analyze the distribution of the Foc races in Taiwan. This will help to control the world-wide important wilt disease of banana. The developed technology platform will be further used for molecular identification and detection of the Fusarium wilt pathogens infecting other economically important crops
