計畫目標：在亞洲蔬菜研究發展中心使用之疫病菌菌株與抗感性之番椒、番茄品種，進行病原性測試與生理小種區分，選拔出抗、感病之番茄與番椒品種以及對應之毒力與非毒力菌系。進一步建立番茄、番椒抗感病品系與疫病菌分子圖譜，進而篩選分子標記。並以非毒力菌株接種抗病品系、毒力菌株接種感病品系分析其不親合反應及親合反應在不同時期基因表現之差異；同時構築番椒、蕃茄親合、不親合反應與之cDNA基因庫，進一步分析基因庫之分子特性。架構(重要工作項目)：在亞洲蔬菜發展中心便用之疫病菌菌株與不同品種之茄科植物，進行病原性測試與生理小種區分，選拔出抗疫病菌、感病之番茄與番椒品種以及對應之毒力與非毒力菌系。建立茄科作物疫病菌分子 標記圖譜，篩選與非毒力基因連鎖之分子標記，建立各菌株之RAPD等圖譜，應用混合分離分析(bulked segregant analysis)進行篩選與非毒力基因連鎖分子標記。並探討不親合反應中的基因表現差異，構築cDNA基因庫並加以分析其意義。預期效益：a.毒力、非毒力菌株遺傳差異圖譜之建立。b.篩選非毒力基因連鎖之分子標記。c.抗感病番椒、番茄品系之基因型分析。d.比較不親合反應中的基因表現差異。Aims of this project: According to pathogenicity and virulence assays with various tomato and pepper cultivars inoculated with P. capsici isolated from AVRDC, we can obtain resistant, susceptible plants and virulent, avirulent races. Then we set up the molecular markers and map the avirulence genes-linked to these DNA markers. Additionally, we use biotechnology with a number of compatible and incompatible interactions to determine that different expressed genes between susceptible and resistant plants. Meanwhile, we want to construct the partial cDNA libraries of peppers and tomatos in the compatible and incompatible reaction for plant disease resistance genes isolation. Frame: we select various cultivars from AVRDC and inoculate with Phytophthora infestans and P. capsici isolates to obtain resistant, susceptible plants and virulent, avirulent races. With map-based cloning strategy, we map avirulence genes with DNA markers and pre-select markers using bulked segregant analysis. We also construct the the partial cDNA libraries of pepper and tomato in the compatible and incompatible reaction and use cDNA analysis to establish gene expression profiles. Prediction and prospect: 1. Establishment of molecular markers of virulent and avirulent race. 2. Selecting molecular markers linked to avirulence genes. 3. Analysis of resistant and susceptible tomato and pepper cultivars' genotype. 4. Gene expression profiles with some biotechnological methods
