洋桔梗為重要切花作物, 田間調查發現有疑似病毒感染之病株. 本計畫擬就該病毒進行分離純化等工作, 並對病毒之基本化學及生物性狀進行探討. 同時製備病毒之抗血清與分子檢測工具, 以建立快速偵測及診斷系統, 並有助於健康種苗驗證制度之建立.Lisianthus plants showing necrotic ringspots and chlorotic spots on leaves were found in the fields. The electron microscopic data showed virusparticles exist inside the diseased lisianthus and thought to be the possible causal agents of symptoms described. In the proposed study, diseased samples will be collected for virus isolation and purification. With purified virus preparations, the basic shemical and biological properties will be investigated. The purified virus samples will also be used to immunie rabbits for polyclonal antibody production. The produced antisera will be used to establish rapid detection techniques for viral infection. The data obtained from this study will also be useful to establish certification system for healthy germplasms of lisianthus
