Studies on Growth and Fruit Quality of Tissue Culture ''Kyoho'' Grapevines

Abstract

摘要 為探討巨峰葡萄組織培養植株之生長及果實品質與扦插植株之差異,本研究以樹勢相近之4年生植株作為調查對象,在中興大學葡萄中心、南投縣竹山鎮及草屯鎮、彰化縣埔心鄉及大村鄉等地,各選擇組織培養植株及一般扦插植株之葡萄園各一處,共12園。調查前先檢測供試植株之病毒感染情形,再調查植株之生長情形及分別在夏季、冬季果實採收時調查分析其果實品質。 由調查結果得知,組織培養及扦插植株部份雖受病毒之再感染,但組織培養植株無論在根、主幹、枝梢、葉片之生長上,均優於扦插植株。在夏果及冬果之果實品質上,組織培養植株之果實大小及著色皆明顯優於扦插植株,至於果實硬度、可溶性固形物及酸度則無明顯的差異。另外由果肉組織之切片發現組織培養者之果肉細胞層數明顯多於扦插者。Summary The differences in growth and fruit quality between 4-year-old tissue culture and cutting ''Kyoho'' grapevines (Vitis vinifera L Vitis labracana Bailey) were investigated. Virus infection status of the plant material was examined before investigation. Most examined plants were infected with virus. However, the growth of tissue culture vines, in terms of roots, trunk, arms, canes, shoots and leaves, were stronger than cutting ones. The berry size and skin color of tissue culture vines were larger and darker than those of cutting ones. There were no significant differences in fruit firmness, total soluble solids and juice acidity. In addition, the number of cell layers of berry flesh that harvested from tissue-culture vines was significantly larger than thet of cutting vines.目錄 一、前言……………………………………………………………1 二、前人研究………………………………………………………3 三、材料與方法……………………………………………………7 四、結果……………………………………………………………11 五、討論……………………………………………………………43 六、中文摘要………………………………………………………48 七、英文摘要………………………………………………………49 八、參考文獻………………………………………………………5

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