目的观察复方风柜斗草对四氯化碳(CCl4)引起的小鼠急性肝损伤的保护作用。方法将小鼠随机分为空白组、模型组、复方风柜斗草低剂量组(2.2 g/kg)、中剂量组(4.4 g/kg)、高剂量组(8.8 g/kg)和联苯双酯阳性对照组(0.2g/kg)。空白组与模型组给予1%羧甲基纤维素钠(CMC-nA)水溶液,其他组给予相应的药物,1次/d,连续灌胃给药10 d后,除正常组外,腹腔注射CCl4致小鼠急性肝损伤。检测肝组织中的超氧化物歧化酶(SOd)及血清中丙氨酸氨基转移酶(AlT)、天冬氨酸氨基转移酶(AST)的活性,同时检测血清中总胆红素(T-bIl)、直接胆红素(d-bIl)及肝组织中丙二醛(MdA)的水平,并计算肝脏指数。结果预先给予复方风柜斗草能显著降低急性肝损伤小鼠体内T-bIl、d-bIl、AlT、AST和MdA的水平,并升高肝组织中SOd活力。结论复方风柜斗草对CCl4致小鼠急性肝损伤具有保护作用,其机制可能与其所具有的抗脂质过氧化和清除体内过多氧自由基的作用有关。Objective To observe the protective effects of compound sarcopyramis nepalensis wall against tetrachloride( CCl4)-induced acute hepatic injury in mice.Methods The mice were randomly divided into normal group,model group,low dose compound sarcopyramis nepalensis wall group( 2.2 g / kg),medium dose group( 4.4 g / kg),high dose group( 8.8 g / kg),and positive control( biphenyldicarboxylate) groups( 0.2 g / kg).The mice were ig pretreated with compound sarcopyramis nepalensis wall and biphenyldicarboxylate once daily for consecutive 10 d,respectively.The mice in the normal and model groups were given 0.1% CMCNa,the water-solution instead.Then,acute liver injury in mice was induced by ip injection of CCl4.The activities of serum ALT and AST,hepatic SOD,and the level of serum T-BIL and D-BIL,hepatic MDA were also determined,the hepatic indices were calculated.Results The activities of ALT and AST,the level of T-BIL,D-BIL and MDA were significantly decreased by compound sarcopyramis nepalensis wall pretreatment,while activity of SOD in hepatic tissues was markedly increased.Conclusion Compound sarcopyramis nepalensis wall has protective effects against CCl4-induced acute hepatic injury in mice,and the mechanisms might be associated with its anti-oxidative and scavenging free radical activity
