目的建立扶正平消胶囊中苦杏仁苷、芍药苷、贝母素甲、黄芪甲苷和吴茱萸次碱5种成分的HPlC-MS测定方法。方法色谱条件:色谱柱为AgIlEnT EClIPSE PluS C18柱(250 MMx4.6 MM,5μM),乙腈-0.1%甲酸水为流动相,梯度洗脱;质谱条件:采用电喷雾离子源(ESI),正离子检测,SIM模式。结果苦杏仁苷、芍药苷、贝母素甲、黄芪甲苷和吴茱萸次碱的定量限分别为12.9、32.2、1.00、1.21、0.40 ng/Ml,检测限分别为6.46、6.44、0.25、0.61、0.16 ng/Ml;在相应的线性范围内r>0.999 0;峰面积之比的日内精密度(rSd)和日间精密度(rSd)均小于2%,平均回收率均在98%~102%。结论本方法可同时测定扶正平消胶囊中苦杏仁苷、芍药苷、贝母素甲、黄芪甲苷和吴茱萸次碱的量,快速简便,实用性强。Objective To simultaneously determine the contents of five components(amygdalin,paeoniflorin,peimine,astragaloside IV and rutaecarpine) in Fuzheng Pingxiao Capsula by LC-MS.Methods Chromatographic separation was achieved with gradient elution by Agilent Eclipse plus C18 column(250 mm × 4.6 mm,5 μm) and an Agilent 1100 Mass Spectrometer system was operated under the SIM mode with electrospray positive ionization(ESI).The mobile phase is acetonitrile-0.1% methanoic acid.Results The LOQ of amygdalin,paeoniflorin,peimine,astragaloside IV,and rutaecarpine in Fuzheng Pingxiao Capsula were 12.9,32.2,1.00,1.21,and 0.40 ng/mL,and the LOD were 6.46,6.44,0.25,0.61,and 0.16 ng/mL,respectively.Within the linear range,r > 0.999 0.Both intra-day and inter-day precision with RSD was less than 2%.The average recovery rates of the five components were in the range of 98%—102%.Conclusion This method is fast,sensitive,and reproducible.It could be used to determine amygdalin,paeoniflorin,peimine,astragaloside IV,and rutaecarpine in Fuzheng Pingxiao Capsula under the same chromatogram condition
