应用rAPd 技术检测经低能氮离子注入甜菊纯系种子引起的幼苗基因组dnA 变异。筛选出OPJ系列中的15 种引物对实验及对照基因组dnA 进行了PCr 扩增，共获扩增片段103 条，分子量在0.3 - 3kb 之间，其中5 种引物OPJ- 1 ，7 ，9，11 ，12 扩增出差异片段12 条。结果表明，低能氮离子注入甜菊种子可引起体内基因组dnA 发生突变；rAPd 技术是检测基因组dnA 发生诱变的一种简便、有效方法。本文同时探讨了离子强度和TAg dnA 聚合酶用量对甜菊rAPd 分析结果的影响，以及氮离子注入诱变效应的可能机制。The paper reports the genomic DNA variation induced by N+ ion implantation in Stevia seeds using RAPD analysis.The total of 103 bands has been amplified from Stevia genomic DNA with fifteen screened primers.The molecular weight of every amplified fragment is between 0.3kb and 3kb.There are twelve polymorphic bands have been amplified by four 10bp primers (OPJ-1, 7,9,11,12).The result show that implantation of N+ ion beam can induce genomic DNA variation.RAPD analysis is a simple and efficient method in detecting genomic DNA variation.The effect of different ion intensity and the quantity of Tag DNA polymerase on the result of RAPD analysis as well as the possible mechanism of biological effects induced by N+ ions implantation is also discussed.国家自然科学基金!重大项目（19890300号
