Pr10(病程相关蛋白10)类蛋白与植物的抵御外来病害及系统获得性抗性(SAr)有着紧密联系,而且许多Pr10类蛋白都具有rnASE活性,并通过这种活性抵抗外源病害。根据获得的XIOSPr10基因,将其构建到PET28A中,通过原核表达及磁珠纯化获得目的蛋白,通过rnA消化实验证实XIOSPr10重组蛋白具有rnASE活性,进一步揭示了XIOSPr10蛋白的功能。It has been regarded that PR10s played an important role in systemic acquired resistance(SAR).Furthermore,many PR10 proteins have been reported to exhibit RNase activity and predicted to be responsible for its antibiotic activity.So the Prokaryotic Expression Vector pET28a-XIOsPR10 was constructed and transferred to BL21(DE3).The recombinant protein XIOsPR10 was obtained by Prokaryotic expression and purification of magnetic microbeads,and showed the RNase activity.国家“863”计划(2007AA10Z132);国家科技重大专项(2008ZX08001-001、2009ZX08009-045B);教育部科技研究重点项目(01102)资助项
