穿刺芽孢杆菌(bACIlluSPEnETrAnS),国外又称穿刺巴斯德氐柄菌(PASTEurIAPEnETrAnS),是根结线虫(MElOIdOgynESPP.)等多种植物寄生线虫的专性寄生菌。作者在我国发现穿刺芽孢杆菌,为了开发利用这种细菌,研制出了每克含3.1x10--8个孢子的菌剂,并就该菌剂对根结线虫的防治效果进行了研究,现将结果报道如下。In order to make use of B.penetrans the preparation of these bacteria was prepared successFully.Each gram of the specimen contains more than 3.1 × 108 spores according to determination by bloodcount plate.The method of its production are: First, inFect the larvae of root-knot nematodes with the spores of B.penetrans.Second, inoculate inFectable plants with the inFected larvae.Third, beat the root-systems of inoculated plants aFter adding some water.The Flowed liquid From a bronze sieve was mixed with the clear water, Filtered, precipitated, air-dried under 5-10cccccc, and grinded into Fine powder, When these powder was added into the water which contained the larvae of root-knot nematodes, the spores of B.penetrans adhered to the cuticles of these larvae in 24h.The pot experiments of tomato, chilli and cockscomb showed that the galls, egg-sacs, Females, males and all-stages of larvae produced by the plant inoculated with the larvae inFected with B.penetrans are about 75%- 80% less than that without these bacteria.Thus it can be seen that the spores of B.penetrans can depress the populations of root-knot nematodes obviously.ThereFore, it is a promising preparation For biological control.国家自然科学基金;福建省自然科学基金;福建省经济发展总公司的资
