细胞编程死亡（PrOgrAMEdCElldEATH）是细胞生理性死亡的一种主要形式。bAX具有抑制细胞编程死亡的作用。本研究采用PCr方法，从人肿瘤细胞Hl—60CdnA文库中扩增出若干个bAXCdnA片段，然后将它们分别与PgEM—T测序质粒载体连接，并转化到大肠杆菌JM109中去。用蓝/白法筛选重组菌落，经酶切分析及PCr鉴定，获得了插入片段大小约为0.4kb及1.1kb的bAXCdnA重组质粒PbAXl和PbAX2。这些片段的测序及表达工作正在进行之中。Programmed cell death is a major way of cell physiological death, and overexpressed Bax gene, one of programmed cell death associated genes.accelerates the progress of programmed cell death.In this paper, three kinds of bax cDNA Fragments were ampliFied From HL-60 cell line by PCR, ligated with PGEM-T directly, and transFormed into E.Colt JM109.Screening with blue-white method and identiFying by restriction endonuclease analysis and PCR, two recombinant plasmids, pBaxl, which contains a bax cDNA Fragment about 0.4kb, and pBax2, which contains a bax cDNA Fragment of 1.1kb, were obtained respectively.Sequencing and expression of bax cDNAs are currently in progress
