用60COγ射线辐照水稻保持系V20b成熟花穗，在不育系V20A上授粉，观察雄配子体双受精行为的辐照剂量效应。结果表明，雄配子体双受精进程所需时间随辐照剂量增大而延长，且主要表现在雌雄配子体核仁融合时间延长。0.186kgy以下剂量组，其延长时间在13H以下，0.279kgy以上剂量组，其延长时间达15H以上。同时，产生多种形态的畸形胚细胞和胚乳核。The mature panicles of rice were treated with 60Coγ-rays in the range of 0～0.372 kGy.The male sterile line used as the Female plants were Fertilized with γ-irradiated pollen manu-ally.The dose eFFect of the irradiated pollen on double Fertilization was investigated.It wasFound that double Fertilization of the irradiated pollen was suppressed to diFFerent degrees ascompared with the control.The eFFect was noticeable as that the Fuslon time of the male nu-cleolus with the Female one was delayed with the increasing of γ-radiation dose.The delayedtime was less than 13 hours when the dose was below 0.186 kGy and it was more than 15hours when the dose was above 0.279 kGy.Furthermore, several types of deFormed embry-onic cells and endosperm nuclei were observed.国家和福建省自然科学基
