本文应用rAPd技术，选用11种引物，以嗜酸乳杆菌为对照，对6种13珠双歧杆菌菌株基因组dnA进行PCr扩增，分析其dnA指纹图谱，并计算其相似性指数。结果表明，双歧杆菌和非双歧杆菌之间，其相似性指数有显著差异；选择合适的引物进行扩增，双歧杆菌不同种间和同种不同株间可表现不同的dnA指纹图谱。本文还对rAPd技术应用于双歧杆菌分类鉴定的可能性进行讨论。11 random primers were chosen to ampliFy the aegomic DNA From 13 strains belonged to 6 species of BiFidobacteria.Lactobacillus acidophillus and Escheichia coli were used as control.AFter analysing DNA Fingerprinting pattems and calculating similarity index(Sl),we Found that the diFFerence of Sl between BiFidobacteria and non-BiFidobacteria was signiFicant and that diFFerent species or strains BiFidobacteria showed diFFerent DNA Fingerprinting patters using appropriate random primers.The possibility of using RAPD For BiFidobacteria idenFiFcation qas discussed
