易位子相关蛋白δ(TrAnSlOCOn ASSOCIATEd PrOTEIn dElTA,TrAPd)是作为内质网膜蛋白参与细胞内信号序列识别、新生肽链的修饰、转运通道的形成等过程。以虹鳟鱼(OnCOrHynCHuS MykISS)为研究材料,采用rT-PCr和rACE法分离和克隆虹鳟鱼TrAPd基因CdnA的全长序列(gEnbAnk登录号:fJ591154),序列全长949 bP,其中5’端非翻译区(uTr)长109 bP,3’端非翻译区(uTr)长336 bP,开放性阅读框(Orf)长504 bP,编码167个氨基酸,存在1个跨膜区。虹鳟TrAPd蛋白序列与斑马鱼、黑青斑河豚、大西洋鲑、斑点叉尾鮰、热带爪蟾、人和小鼠等脊椎动物的同源性均在80%左右,表明TrAPd基因在进化过程中是高度保守的。Translocon-associated-protein delta(TRAPD) plays a crucial role in physiological processes such as the recognition of signal sequence,the modification of nascent peptide and the formaction of translocation channels.RT-PCR and RACE(Rapid Amplification of cDNA Ends) methods were used for the isolation of the whole cDNA of trapd gene(GenBank accession number: FJ591154) from rainbow trout.Sequence analysis revealed a 949 bp cDNA containing the 109 bp 5'-UTR,336bp 3'-UTR and 504 bp ORF encoding 167 amino acids with a transmembrane region.The sequence alignment between rainbow trout and zebrafish,fugu,salmon,catfish,frog,human and mouse exhibited about 80% identity rate of amino acid.The result indicated the trapd gene is highly conservative in the progress of evolution.河北省科技厅资助项目(0624240011
