Detection and analysis on the secretions of Oncomelania hupensis snails dually infected by larval Exorchis mupingensis and Schistosoma japonicum at different intervals
观察被目平外睾吸虫和日本血吸虫双重感染钉螺后钉螺体内外分泌物及其血吸虫幼虫被击毁的关系。方法观察钉螺感染外睾吸虫21d、37d、55d、70d和85d后再感染血吸虫,经4~82d后,取钉螺软体组织作埋蜡连续染色制片。结果与结论无论单独感染目平外睾吸虫或两种吸虫双重感染,均可在螺软体发现多种分泌物,随时间延长分泌物数量逐渐增多。螺体内含小细胞核的分泌物和螺副腺细胞都出现在各时期的血吸虫残骸体内。The attack degrees on larval S.japonicumin O.hupensis snails dually infected by Exorchis mupingensis and S.japonicum with longer intervals were stronger than that of shorter intervals,and snail haemo-lymphocytes numbers were more few in that of shorter intervals.Present experiment would like to observe how about the actions of secretions of O.hupensis with different intervals took place to the larval S.japonicum wreckages.To examine snails' secretion status,all experimental snails were processed by paraffin-embedding methods,sectioned and stained with hemotoxilin and eosin;sectioned snails were examined using light microscopy.Although the numbers of 3species hemo-lymphocytes were fewer in O.hupensis snails dually infected with longer intervals than that of shorter intervals,the secretions of snails were thicker and more in O.hupensis snails dually infected with longer intervals than that of shorter intervals.How about the effect of snail's secretions on the S.japonicumlarvae and how about the change of snail's immunological defencing system in O.hupensis snails dually infected by Exorchis spp.and Schistosoma japonicum require more detailed further investigation.国家自然科学基金(No.31270938)资助~
