利用ISSr分子标记对来自10个不同生态地区的红锥优良种质进行基因组遗传多样性分析.从20条引物中筛选出11条具有扩增多态性的引物用于ISSr-PCr扩增,共扩增出435条带,其中多态性条带325条,占总扩增条带的74.7%,平均每个引物扩增3.95条.ISSr标记分析揭示出红锥10个优良种质的遗传相似系数介于0.473 7--0.808 1之间,平均值为0.728 7.用STATIS统计软件的欧氏可变类平均法构建类型之间的分子系统树,聚类分析结果将10个优良红锥种质分为4个类群.Genetic diversity of fine germplasm resources of Castanopsis hystrix from ten different ecological regions was analyzed by using the inter-simple sequence repeat(ISSR),and 11 effective primers were selected from 20 primers.The result showed as follows: 435 bands were amplified,among which 325 bands were polymorphic,and percentage of polymorphic bands were 74.7 %,an average of approximately 3.95 bands per primer.Genetic similarity indexes of ten germplasms of C.hystrix ranged from 0.473 7 to 0.8081,and the average value was 0.728 7.Static software was used to calculate the Nei's genetic distance and a dendrogram was constructed based on UPGMA cluster analysis.These ten germplasms were divided into 4 major groups,and their genetic relative and the genetic distance were discussed.广西林业科学研究院国家林业局中南速生材繁育重点实验室开放基金课题;兰州理工大学博士基金(SB08200602);甘肃省教育厅研究生导师基金(0703-11
