目的:研究维甲酸对人成骨肉瘤Mg-63细胞增殖和相关基因表达的影响,以探索其对成骨肉瘤细胞的生物学效应。方法:以1μMOl/l维甲酸处理人成骨肉瘤Mg-63细胞,生长曲线测定,流式细胞仪分析、光镜观察和免疫细胞化学检测等研究维甲酸对Mg-63细胞的生长曲线、细胞周期和相关癌基因、抑癌基因表达的影响,并对其作用机理进行初步分析。结果:维甲酸处理7天后,Mg-63细胞生长抑制率达到42.2%,g0/g1期比例达到61.8%,细胞形态铺展,排列趋于规则,癌基因C-MyC、C-fOS的表达降低,而抑癌基因rb、P27表达上调。结论:1μMOl/l维甲酸可以有效抑制细胞的增殖活动,改变细胞恶性形态特征,下调癌基因C-MyC、C-fOS和上调抑癌基因rb、P27的表达,从而对人成骨肉瘤细胞分化具有诱导作用。Objective:To explore effect of Retinoic Acid(RA) on proliferation of human osteosarcoma MG-63 cells and its mech-anism of antitumor.Methods:The MG-63 cells treated by 1μmol/L RA were subjected to growth curve counts,flow sytometry,im-muncocytochemical assay and light microscopy.We investigated the influence of RA on proliferation,cell cycle,expression of associated oncogene and tumor suppressor gene of MG-63 cells.Then we analyzed the mechanism of RA in inducing differentiation primarily.Re-sults:Seven days after treated by 1 μmol/L RA,the inhibition rate of proliferation of MG-63 cells amounted to 42.2 % and RA reduced the rate of progression from G1 to S phase.The cells tend to be flat and spread.Meanwhile,the expression level of oncogene was down-regulated and the expression level of tumor suppressor gene was upregulated.Conclusions:1μmol/L RA could inhibit proliferation of MG-63 cells,reverse the malignant phenotype characters,downregulate expression of Oncogene c-Myc,c-fos and upregulate expression of Tumor Suppressor Gene Rb,p27,as a result,induce MG-63 cells into differentiation effectively.国家自然科学基金资助项目(30470877
