以能够有效抑制塔玛亚历山大藻生长的海洋细菌VIbrIO SP.dHQ25为对象,考察了培养基成分对其发酵生长的影响.研究中顺序利用了PlACkETT-burMAn(Pb)设计法、最速上升法以及中心组合响应曲面方法对培养基组成分进行优化,获得最适培养基配方(g/l):蛋白胨13.24,酵母粉4.31,葡萄糖0.1,nACl 25,MgSO4.7H2O 0.2,CACl2.2H2O 0.5,kCl 0.06,k2HPO40.05,kH2PO40.05,fE2(SO4)30.002.由最适配方发酵得到的dHQ25培养液的菌液浓度(Od600)平均可达1.984,较原先2216E培养基中培养的1.359提高了46%,杀藻效果与生长密度间存在着特定的相关性,这为今后分离杀藻活性物质提供了基础.结果表明,Pb设计法与响应曲面法的结合使用能经济有效地对该杀藻细菌的高密度生长培养基成分进行优化.The influence of medium compositions on the growth of marine algicidal bacteria Vibrio sp.DHQ25 was investigated.Plackett-Burman(PB) design,steepest ascent design and central composite design were orderly employed to obtain the optimized medium in this study.The chemical defined medium per liter contains(g/L):13.24 peptone,4.31 yeast extract,0.1 glucose,25 NaCl,0.2 MgSO4·7H2O,0.5 CaCl2·2H2O,0.06 KCl,0.05 K2HPO4,0.05 KH2PO4,0.002 Fe2(SO4)3.The optical cell density at 600 nm(OD600) could reach 1.984,which was increased by 46% compared with 1.359 of that in 2216E medium,the algicidal activity was related to cell density to some extent.This will be beneficial to the further study on the isolation of algicidal compound.It indicated that the combination of Plackett-Burman design with response surface metholodology could effectively optimize the high cell density medium compositions of this marine algicidal bacteria.国家自然科学基金重点项目、主任基金项目和面上项目(40930847;31070442;30940002;40876061);国家高技术研究发展计划(863)项目(2008AA09Z408);长江学者和创新团队发展计划项目(40821063);近海海洋环境科学国家重点实验室资助项
