运用液质联用技术建立了水产干制品中40种抗生素的同时测定方法。含0.1%甲酸乙腈进行提取,考察了不同萃取溶剂及nA2EdTA添加量对目标抗生素回收率影响。目标抗生素在0.1~100μg/kg范围内线性良好;以基底匹配工作曲线进行定量,在高加标浓度下虾仁、鳗鱼干、红娘鱼干的加标回收率和rSd(n=4)分别为40.5%~138.6%和0.4%~19.4%、41.3%~134.5%和1.3%~11.6%、及40.3%~136.7%和0.1%~14.7%,方法检测限在0.1~0.5μg/kg。方法快速、简便,已成功应用于厦门周边常见水产干制品的检测。A method for simultaneous determination of 40 antibiotics in dried aquatic products was established using liquid chromatography tandem mass spectrometry(LC-MS/MS).Effects of experimental conditions,such as extraction solvents and Na2 EDTA adding on extraction efficiency were investigated.Under the optimized conditions,recoveries of 40 target drugs calculated by matrix match calibration curve at 20 μg/kg spiking level and RSD(n=4)were 40.5%~138.6% and 0.4%~19.4% for dried shrimp,41.3%~134.5% and 1.3%~11.6% for dried ell and 40.3%~136.7% and 0.1%~14.7% for dried gurnard,respectively.Most of method detection limits of target antibiotics ranged from 0.1 to 0.5 μg/kg.The method was sensitive,accurate,fast and successfully applied in the analysis of residual antibiotics in typical aquatic products in Xiamen
